Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 Cellar session 9 Mannoprotein extracts from wine lees: characterization and impact on wine properties

Mannoprotein extracts from wine lees: characterization and impact on wine properties

Abstract

This study aims at exploiting an undervalued winemaking by-product, wine yeast lees, by developing efficient and food-grade methods for the extraction of yeast glycoproteins. These extracts were then supplemented to wine and their impact on wine properties assessed. White wine lees were produced by fermenting Sauvignon blanc grape juice with S. cerevisiae Uvaferm HPS strain. Three extraction methods were applied on lees using physical (autoclave and sonication) or enzymatic (Glucanex®, an industrial β-glucanases) approaches. Glycoproteins extracts were characterized by SEC-HPLC and SDS-PAGE. After their addition to wine (0.5 g/L), no alteration of wine clarity was detected. The ultrasonication and enzymatic extracts, containing a relatively low amount of glycoproteins, led to a significant decrease in wine protein haze formation upon heat test (-7%). Conversely, the autoclave extract was the richest in glycoproteins and had a positive impact on wine foaming properties, inducing an increase in foam’s maximum height and stability which were 2.6 and 3.6 times higher compared to a model wine. The autoclave extract improved tartrate stability as shown by a decrease in wine conductance (-11%) compared to the untreated wine. Results suggest that white wine lees could be considered a valuable source of glycosylated proteins with potential applications in winemaking. In this context, the autoclave appears as the more promising method in terms of both efficiency and extract’s effectiveness. The proposed food-grade exploitation approach could represent an important tool to improve the environmental and economical sustainability of the wine supply chain.

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Alberto De Iseppi1, Andrea Curioni1,2, Giovanna Lomolino1, Matteo Marangon1, Simone Vincenzi1,2 and Benoit Divol3

1Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università, 16, 35020, Legnaro, Padova, Italy
2Centre for Research in Viticulture and Enology (CIRVE), Viale XXVIII Aprile 14, 31015, Conegliano, Italy
3South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

Contact the author

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

ePROSECCO: Historical, cultural, applied philosophy analysis and process, product and certification innovation, for the “sustainable original progress and promotion 4.1c” of a historic and famous territory and wine

According to the algorithm “A step back towards the future 4.1C”, (Cargnello,1986a, 1987d, 1988a.b, 1991, 1993, 1994b, 1995, 1999a.e, 2000b, 2007c, 2008a, 2009d, 2013; and according to the principles of “Charter of Sustainable Viticulture BIO‐MetaEthics 4.1CC” of GiESCO (Carbonneau and Cargnello, 2003 2015, 2017), the historical, applied philosophy and productive analysis connected to the innovations and to the “Certification of the Universal Holistic MetaEthical Sustainability 4.1C” “indexed new global production model 4.1C” has always been fundamental, especially for the “Prosecco Territory” and for the “Prosecco Wine” to design and implement their synergistic future “Sustainable and Certificable 4.1CC” according to the principles of the “Charter of Sustainable Viticulture BIO‐MetaEthics 4.1CC” by the GiESCO (Carbonneau and Cargnello, lc, Cargnello et Carbonneau, 2007, 2018), and of the Conegliano Campus 5.1C. (Cargnello, lc). Nowadays, people think that Prosecco is a wine from the Veneto Region (from Conegliano and Valdobbiadene in particular), while it comes from Friuli‐Venezia Giulia Region (in North Eastern Italy, such as Veneto) more precisely from “Prosecco” in the Municipality of Trieste (TS‐Italy), as documented in 1382 and in 1548, when Pier Andrea Mattioli, described “that ancient wine, which is born in Prosecco”, as a wine with the following characteristics “thin, clear, shiny, golden, odorous and pleasant to taste». In 1888 at the “Wine Fair” of Trieste there were the “Sparkling wine Prosecco” by Giovanni Balanc, by Giuseppe Klampferer and that one by Marino Luxa. In the 19th century, many expressed their appreciation for the “Prosecco” of Trieste. In order to implement intra and extra territorial and cross‐border relations, as well as, the “Certification of: Products, Companies, Territory, Bio‐MétaÉthique District 4.1C” of Prosecco, a series of activities and researches were conducted in 8 companies: 5 in the “Territory of Prosecco” (TS) in which the principles of “Charter of Sustainable Viticulture BIO‐MetaEthics 4.1CC” of GiESCO (Carbonneau and Cargnello, lc) have been successfully applied. In particolar: 1‐ new and original “Sustainable 4.1C global production model” developed also to prevent the problems caused by wild boar, roe deer, and birds while safeguarding their “psychophysical wellness”, as well as the “psychophysical wellness 4.1C” of the macro and micro flora and fauna, of the biodiversity, of the landscape, etc. (Cargnello, lc), 1.2‐ chemical weed control and “Non MetaEthics 4.1C” processing with the total grass growing of the ground without or with mowing, better if it is manual to protect grass, air and soil, 2‐ recovery of “Historic”: land, vineyards, vines, biodiversity, landscapes, productions, products, … , 3‐ production of the famous “Prosekar, also rosé, of Prosecco” and “Prosecco di Prosecco”, according to “A step back towards the future 4.1C” 4‐ to offer a deserved psychophysical well‐being to the “Prosecco Territory” and entrepreneurs. 

The effect of soil and climate on the character of Sauvignon blanc wine

Un projet multidisciplinaire sur l’effet du sol et du climat sur la qualité du vin a débuté en Afrique du Sud il y a 5 ans. Des mesures sont effectuées sous culture sèche dans des vignes de Sauvignon Blanc dans six localités différentes, cinq dans le district de Stellenbosch et une à Durbanville.

Gastrointestinal digestion of wine sulphites and their effects on human gut microbiota

Sulphites are by far the most widely used additive in the wine industry. In relation to health, the interaction of sulphites with the gut microbiota has not been addressed so far. Following the consumption of wine and other sulphite-containing foods, the gastrointestinal tract and the microbiome are one of the first barriers that these compounds face in the human organism. In this study, we used a previously validated gastrointestinal digestion model (SIMGI®) [1,2] to evaluate the effect of intestinal digestion of wine sulphites on the gut microbiome.

The estimation of the clear-sky effective PAR resources in a mountain area

Irrigation of vineyards is a matter of controversial arguments at areas of high quality wine production. Besides, the effects of the water in the plant are closer related to the water availability than to the irrigation regime.

Histoire des Vitis depuis leurs origines possibles sur la Pangée jusqu’aux cépages cultivés : un exemple de résilience liée à la biodiversité des espèces

The first forms of life on earth were bacteria and single-celled blue-green algae. They evolved into land plants around 500 million years ago, developing mechanisms for surviving on land, such as roots, stems and leaves. This evolution also led them to coexist with other organisms, such as insects and animals, for pollination and seed dispersal, as well as to resist environmental factors such as drought and disease.