Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 qNMR metabolomics a tool for wine authenticity and winemaking processes discrimination

qNMR metabolomics a tool for wine authenticity and winemaking processes discrimination

Abstract

qNMR Metabolomic applied to wine offers many possibilities. The first application that is increasingly being studied is the authentication of wines through environmental factors such as geographical origin, grape variety or vintage (Gougeon et al., 2019). Another less common approach is from a qualitative point of view by studying the various oenological practices used that are an integral part of the elaboration of a wine. We wondered whether quantitative NMR could be used to dissociate the physical or chemical processes commonly used in oenology. The objective of this work was to provide a better understanding of the interactions between oenological processes and wine by determining the metabolites responsible for differentiation through 1H-NMR fingerprinting and chemometrics. 

About 40 molecules were quantified on wine samples that have undergone several physical and chemical processes. Principal Component Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA) and S-score were performed for the analytical discrimination of winemaking processes. 

The results obtained show that qNMR combined with chemometrics allows to dissociate not only physical processes such as filtration, but also chemical processes such as maceration temperature, enzymatic treatment and fining. In addition, the metabolites involved in the discrimination of these winemaking processes could also be determined.

The 1H-NMR metabolomics is a fast technique that could be used as a tool to help professionals decide on technical itineraries. 

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Inès Le Mao1, Gregory Da Costa1, Jean Martin1, Eric Pedrot1, Soizic Lacampagne1, François Guyon2, Tristan Richard1

1Université de Bordeaux, Œnologie EA 4577, USC 1366 INRA, INP, ISVV, 210  chemin de Leysotte, 33882 Villenave d’Ornon, France
2Service Commun des Laboratoires, 3 Avenue du Dr. Albert Schweitzer, Pessac Cedex, France

Contact the author

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

Impact of toasting and botanical origin on oak wood (Q. sp.) volatilome using untargeted GCxGC-ToFMS analysis

Many works have been carried out to identify the key aroma volatile compounds of oak wood (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using conventional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS). Inspired by recent untargeted approaches in the field of food “omics”, this work aims to extend our knowledge on the impact of cooperage process on the volatile composition of oak wood using two-dimensional comprehensive gas chromatography coupled with time of flight mass spectrometry (GCxGC-ToFMS).

New satellite-based sampling protocols for grapevine nutrient monitoring

Extension specialists often recommend nutrient monitoring through leaf blade or petiole sampling twice a season for each vineyard block. However, due to the time and labor required to collect a large, random sample, many growers complete the task infrequently or incorrectly. Readily available remote sensing images capture the vineyard variability at both spatial and temporal scales, which can capture canopy and soil variability and be used to guide growers to representative sampling locations.

Grapevine cane pruning extract enhances plant physiological capacities and decreases phenolic accumulation in canes and leaves 

Vine cane extracts are a valuable byproduct due to their rich content of polyphenols, vitamins, and other beneficial compounds, which can affect and benefit the vine and the grapes. This study aims to evaluate the response of grapevine plants to irrigation with water supplemented with a vine cane extract, both at physiology response and phenolic composition in different parts of the plant (root, trunk, shoot, leaf, and berry).
Cane extract was obtained by macerating crushed pruning residues with warm water (5:1) and pectolytic enzymes. Two-year-old potted plants were irrigated with water (Control) while others were irrigated with cane extracts, either at 1:4 (w/v, cane extract/water; T 1:4) or at 1:8 (w/v, cane extract/water; T 1:8).

Wine yeast species show strong inter- and intra-specific variability in their sensitivity to uv-c radiation

While the trend in winemaking is toward reducing the inputs and especially sulphites, the development of While the trend in winemaking is toward reducing the inputs

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.