 IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Grapevine diversity and viticultural practices for sustainable grape growing 9 Grape texture characteristics are linked to one major qtl

Grape texture characteristics are linked to one major qtl

Abstract

AIM: Berry texture and berry skin mechanical properties have high agronomic importance, related to quality and marketing requirements of wine, table and raisin grapes. Despite the efforts already made to detect molecular markers and candidate genes associated with berry texture, different QTLs were proposed until now, showing low contribution rates to the trait, likely due to difficulty in phenotyping. Searching for QTLs linked to berry texture, an F1 population of 154 individuals and their parents (‘Raboso Veronese’ and ‘Sultanina’) were used in this study.

METHODS: Density sorting by flotation was applied to reduce sample variability. One density class was selected achieving berries with a similar ripening stage. Mechanical properties were measured and normalized on berry diameter, surface, and volume. Hundred and ninety SSR molecular markers were used to produce a genetic map using JoinMap. MapQTL was applied searching for QTLs associated with berry texture traits. VviAGL11 expression profiling and co-expression analysis during grape ripening was evaluated using available transcriptomic datasets.

RESULTS: A major QTL was found on LG 18, with high LOD scores (from 25.07 to 31.92) and high phenotypic variance explained (from 53.2 to 63.5%) for all measured texture traits. Surprisingly, this major QTL showed association with SSR markers linked to VviAGL11, the main gene leading to stenospermocarpy. Data available on VviAGL11 expression and co-expression profiling during grape ripening strongly suggested that this gene may act on the traits of a ripe berry through the activation of some target genes involved in lipid and hormone metabolism, transport and in gene expression regulation.

CONCLUSIONS

Previous studies showed how difficult is determining the genetic control of berry texture. Our results clearly underline the major role played by a QTL located on LG18 and characterized by the presence of the well-known MADS-box gene VviAGL11.

DOI:

Publication date: September 2, 2021

Issue: Macrowine 2021

Type: Article

Authors

Manna Crespan

CREA Research Centre of Viticulture and Enology, Conegliano, Italy,Daniele Migliaro, CREA Research Centre of Viticulture and Enology, Conegliano, Italy Silvia Vezzulli, Fondazione Edmund Mach, S. Michele a/Adige, Trento, Italy Sara Zenoni, University of Verona, Italy Giovanni Battista Tornielli, University of Verona, Italy Simone Giacosa, University of Torino, Italy Maria Alessandra Paissoni, University of Torino, Italy Susana Río Segade, University of Torino, Italy Luca Rolle, University of Torino, Italy

Contact the author

Keywords

Vitis vinifera L., fruit quality, flotation, lg18, vviagl11, mads box genes

Citation

INOCULATION OF THE SELECTED METSCHNIKOWIA PULCHERRIMA MP1 AS A BIOPROTECTIVE ALTERNATIVE TO SULFITES TO PREVENT BROWNING OF WHITE GRAPE MUST

Enzymatic browning (BE) of must is caused by polyphenol oxidases (PPOs), tyrosinase and laccase. Both PPOs can oxidize diphenols such as hydroxycinnamic acids (HA) to quinones, which can later polymerize to form melanins [1], which are responsible of BE in white wines and of oxidasic haze in red wines. SO₂ is the main tool used to protect must from BE thanks to its capacity to inhibit PPOs [2]. However, the current trend in winemaking is to reduce and even eliminate this unfriendly additive. Among the different possible alternatives for protecting must against BE, the inoculation with a selected Metschnikowia pulcherrima MP1 is without any doubt one of the most promising ones.

Zonazione dell’area viticola doc durello

Il lavoro di zonazione riveste un ruolo importante per capire le potenzialità e la vocazionalità di una specifica area viticola. La viticoltura dovrebbe essere vista in funzione dell’obiettivo enologico che si vuole realizzare e quindi particolare importanza riveste il risultato delle vinificazioni delle uve provenienti dai vigneti delle diverse aree della zona di produzione oggetto d’indagine. La zonazione dell’area a DOC Monti Lessini Durello ha preso in esame la varietà “Durella”, vitigno autoctono del territorio, che rappresenta la maggior parte della produzione vitivinicola della zona.

Effects of winemaking variables on the chemical and sensory quality of Schiava wines up to one year storage in bottle

The interactive effects of three major enological variables were evaluated on the quality of Schiava wine up to one year of storage in bottle.

Évaluation environnementale de pratiques vitivinicoles innovantes

The Institut Français De La Vigne Et Du Vin (IFV) is conducting many experiments on innovative winegrowing practices, which are emerging in companies in the sector, or which are still at the R&D stage for agricultural suppliers. The purpose of these practices may be to reduce environmental impact, to adapt vineyards to climate change, or to achieve other technical, economic or social aims. Whatever the objective, it is necessary to verify the relevance of these new practices, and in particular their environmental relevance, i.e. That at the very least, the changes in practices do not increase the environmental impact of the technical itineraries.

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.