Macrowine 2021
IVES 9 IVES Conference Series 9 Cell-to-cell contact modulates Starmerella bacillaris early death in mixed fermentations with Saccharomyces cerevisiae in a couple-dependent way

Cell-to-cell contact modulates Starmerella bacillaris early death in mixed fermentations with Saccharomyces cerevisiae in a couple-dependent way

Abstract

AIM: The diversity and complexity of the fermentation ecosystem during wine making limits the successful prediction of wine characteristics. The use of selected starter cultures has allowed a better control of the fermentation process and the production of wines with established characteristics. Among them, the use of mixed fermentations with Starmerella bacillaris and Saccharomyces cerevisiae yeasts has gained attention in recent years due to the fructophylic nature of the first and the ability of this inoculation protocol to reduce the acetic acid and ethanol content of the wines. The yeast species interact throughout the alcoholic fermentation and influence the chemical composition of the wines. Many studies have been carried out to gain an insight to the nature of these interactions, with the aim to better control the wine fermentation.

METHODS: In order to understand the population kinetics of Starm. bacillaris in mixed fermentations with S. cerevisiae and to investigate the effect of physical separation of the two species during fermentation, several experimental setups were employed. Importantly, double-compartment fermentation was carried out to elucidate the role of cell-to-cell contact in the death of Starm. bacillaris occurring during mixed fermentations. Furthermore, several strains of both Starm. bacillaris and S. cerevisiae were used, in order to get an insight into these interactions.

RESULTS: The early death of Starm. bacillaris in mixed fermentations in flasks as compared to the double-compartment fermentor, seemed to be not due to nutrients depletion or to the presence of toxic compounds, but most likely due to cell-to-cell contact induced inhibition. Nevertheless, the early death behavior as well as the extension of viability of Starm. Bacillaris in the double-compartment fermentor, depended on the individual strains of the two species tested and their combinations in couples during fermentation. These results lead to the hypothesis that the cell-to-cell contact mechanismdepends greatly on the couple Starm. bacillaris and S. cerevisiae used to conduct the fermentation process.

CONCLUSIONS:

In the future it will be necessary to make further studies in order to better investigate this aspect and to define with greater clarity how cell-to-cell contact mechanism happens and how it is regulated to be able to perform fermentations with different strains combinations and obtain wines with specific characteristics.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Kalliopi Rantsiou, Vasileios ENGLEZOS,  Paola DI GIANVITO,  Luca COCOLIN, Kalliopi RANTSIOU

University of Turin, DISAFA, Italy, University of Turin, DISAFA, Italy

Contact the author

Citation

Related articles…

Cover crops under-vine impact on grapevine performance and vineyard soil microorganisms is highly affected by edaphoclimatic conditions at a regional scale 

Soil management through cover crops can influence the cycle of nutrients, promote water infiltration, decrease erosion, and enhance the soil microbiota biodiversity, improving the grapevine performance. However, the area under the vines tends to be left bare by applying herbicides or tillage to avoid competition with the crop in semi-arid climates. Use of covers under-vine might be an alternative to these practices aiming at grapevine quality and soil health improvement. The aim of this research was to study the implications of soil management under the vines (cultivation and cover crops) on growth, yield, berry composition and soil microbial communities. A cover crop composed by a mixture of legumes was sown and compared with a control (cultivation), which includes frequent tillage to keep the soil bare, in three areas characterized by different edaphoclimatic conditions in the region of Navarra.

Evolution of cabernet sauvignon wines macerated with their own toasted vine-shoots

Toasted pruning vine-shoots represent a promising new enological tool for developing wines with chemical and organoleptic high quality, allowing that the resources of the vineyard to be returned to the wine through a “circular process”.

DNA-Free genome editing confers disease resistance in grapevine

Grapevine (Vitis Vinifera L.), one of the most important cultivated fruit crops, is facing significant challenges due to climate change. Specifically, increasing temperatures negatively impact the physiological traits and disrupt plant phenology. Additionally, increased virulence in pathogen attacks and pests leads to significant yield loss, requiring widespread application of plant protection products. Traditional agronomic practices offer only partial mitigation, requiring the development of precise and effective intervention strategies. The economic worth of viticulture has prompted continuous efforts in grapevine genetic improvement programs, traditionally involving conventional breeding and clonal selection that, however, are complex and time-consuming approaches.

Assessing bunch architecture for grapevine yield forecasting by image analysis 

It is fundamental for wineries to know the potential yield of their vineyards as soon as possible for future planning of winery logistics. As such, non-invasive image-based methods are being investigated for early yield prediction. Many of these techniques have limitations that make it difficult to implement for practical use commercially. The aim of this study was to assess whether yield can be estimated using images taken in-field with a smartphone at different phenological stages.

Water dynamics of Touriga-Nacional grapevines trained in cordon and guyot systems under Mediterranean climate conditions

Aims: The aims of the present study were to (1) evaluate the water dynamics of Touriga-Nacional grapevines trained to spur pruned cordon and Guyot systems and (2) assess the effect of variable water availability in a commercial vineyard located in the Demarcated Douro Region (DDR), Portugal.