Macrowine 2021
IVES 9 IVES Conference Series 9 A microbial overview of txakoli wine: the case of three appellations of origin

A microbial overview of txakoli wine: the case of three appellations of origin

Abstract

The Txakoli, a white wine produced in the Basque Country (North of Spain), has recently gained popularity due to wine quality improvement and increase in both acreages of production and wine consumption. The aim of this study was to characterize the chemical and microbiological differences between Txakoli wines made with grapes from different sites. Our analysis included Txakoli vineyards from three appellations of origin: Bizkaiko, Getariako, and Arabako Txakolina D.O.To describe the microbial composition, we sequenced using next generation sequencing the v4 domain of the 16S rRNA gene from 41 vineyard soils and grapes collected in 2016 and 2017. Metadata information (slope, orientation, soil edaphic properties, grape physical factors, etc.) was also collected and used to identify the potential environmental and factors responsible for the differences in the microbial composition of soils and grapes.Soil pH significantly associated with differences in soil bacterial composition, grouping the 41 vineyards into 5 clusters, regardless of vintage or appellation. The historical land usage of the properties was found to be also a significant factor determining soil bacterial composition. Interestingly, the bacterial composition of grape berries significantly depended on rootstock type, supporting a strong influence of the rootstock genotype on the fruit-microbial associations. When removing rootstock as a factor, sugar content and pH significantly correlated with microbial composition differences between sites, revealing grape maturity as an additional important factor that drives microbial associations in the fruit.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Igor Baroja, Elena GARCÍA DE LA PEÑA, Iratxe ZARRAONAINDIA

University of the Basque Country, Ardoatek Dario, CANTU, University of California, Davis  And one, ESTONBA, University of the Basque Country, University of the Basque Country IKERBASQUE

Contact the author

Keywords

vineyard, microbiome, miseq, wine, txakoli

Citation

Related articles…

Yield formation and grape composition: more than meets the eye 

Fruit quality in grapes is not well defined but is often depicted as correlating inversely with crop yield. Both fruit yield and composition, however, are made from distinct components that interact in complex ways. Reproductive growth of grapevines extends over two growing seasons. Inflorescences initiated in buds during the previous year differentiate flowers and set and develop berries during the harvest year.

Determination of titratable acidity, sugar and organic acid content in red and white wine grape cultivars during ripening by VIS–NIR hy¬perspectral imaging

Grape harvest time is one of the most fundamental aspects that affect grape quality and thus wine quality. Many factors influence the decision of harvest; among them technological and phenolic maturity of grape. Technological ripeness is mainly related to sugar concentration, titratable acidity and pH. Conventional methods for chemical analysis of grapes are normally sample-destructive, time-consuming, include laborious sample preparation steps, and generate chemical waste, thereby limiting their utility in online/in-line quality monitoring. Moreover, destructive analyses can be performed only on a limited number of fruit pieces and, thus, their statistical relevance could be limited. This study evaluated the ability of a lab-scale hyperspectral imaging (HYP-IM) technique to predict titratable acidity, organic acid and sugar content of grapes. Samples of Cabernet franc and Chenin blanc grapes were consecutively collected six times at weekly intervals after veraison. The images were recorded thanks to the hyperspectral imaging camera Pica L (Resonon) in a spectral range from 400 to 1000 nm. Statistics were performed using Microsoft Xlstat software. Successively, the berries were analyzed for their sugar (glucose and fructose) and organic acid (malic and tartaric acid) content and titratable acidity according to usual methods.

How to reduce SO2 additions in wine with the aid of non-conventional yeasts

Among the factors that influence the sensory quality, style, safety, sustainability, and sense of place of a wine, the contributions of microbial biodiversity are widely becoming more recognized. Throughout winemaking, multiple biochemical reactions are performed by a myriad of different microorganisms interacting in many ways.

Influence of must fining on wine pinking: relationship between electrochemical and colorimetric measurements and pinking attitude of wine

“Pinking” is a term used to define an abnormal pink coloration assumed by white wines in certain cases. Despite the are many hypotheses about the causes of this phenomenon, pinking still represents an issue for the wine industry. In the absence of reliable preventive strategies, wineries often rely on treatments such as charcoal fining, which is also negatively impacting wine aroma. This study aims at evaluating the potential of different fining agents based on animal or vegetal proteins to prevent wine pinking when applied at the level of must clarification. The work was carried out on Lugana wines, which is well-recognised as sensible to pinking problems. METHODS: Two experimental Lugana musts were obtained by applying a standard winemaking protocol and were then clarified with different commercial preparations based on vegetal proteins or casein, alone or in combination with PVPP. A control only using pectolytic enzyme was also prepared. Finings were carried out at 4°C for 16h, and the clear must (200 NTU) was then fermented in controlled conditions.

The effects of perennial cover crop management on soil temperature and vine water status

The implications of perennial cover crop management on vine vigor and yield have been well documented. However, whereas multiple studies show that cover crop management affects grapevine dry matter production, water, and nutrient status, the specific effects of a new hybrid perennial cover crop on soil temperature and its relationship to vine water status in vineyards has not been explored. This study will compare 3 different perennial cover crop combinations and tillage practices with a no-till seeding of a new hybrid perennial, Poa bulbosa (Pb).