Macrowine 2021
IVES 9 IVES Conference Series 9 Bacterial community in different wine appellations – biotic and abiotic interaction in grape berry and its impact on Botrytis cinerea development

Bacterial community in different wine appellations – biotic and abiotic interaction in grape berry and its impact on Botrytis cinerea development

Abstract

An in-depth knowledge on the conditions that trigger Botrytis disease and the microbial community associated with the susceptibility/resistance to it could led to the anticipation and response to the Botrytis emergence and severity. Therefore, the present study pretends to establish links between biotic and abiotic factors and the presence/abundance of B. cinerea. Several grape varieties from 4 different wine appellations in France and Spain have been studied at different maturity stages to analyse: 1) B. cinerea abundance (established by qPCR), 2) grape composition parameters (comprising water activity measuring, exudates composition, phenologic stage, gluconic acid, calcium, etc), and 3) grape berries microbial community diversity and composition (using 16S rRNA and ITS amplicon sequencing).Preliminary analysis of the results obtained through 16S rRNA Next Generation Sequencing revealed differences in microbial richness and bacterial composition between the vineyards. Both alpha and beta diversities correlated with fruit maturity, where grapes at harvest stage showed significantly higher richness and a dissimilar bacterial composition. In addition, bacterial community structure differed between wine appellations. The study will increase significantly our understanding of the ecology of microbial associated to different grape varieties and viticulture areas. Additionally, it will generate knowledge about the factors.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Guilherme Martins 1,2, Pauline Mazeau 1, Audrey Barsacq 1, Laurence Geny 1, Isabelle Masneuf-Pomarède 1,2 , Miren Andone Recalde 3, Iratxe Zarraonaindia 3

1 Université de Bordeaux, Isvv, Unité de Recherche Oenologie Ea 4577, Usc 1366 Inrae, Bordeaux Inp, 33140 Villenave D’Ornon, France.
2 Bordeaux Sciences Agro, 33170 Gradignan Cedex, France.
3 Department of Genetics, Physical, Anthropology & Animal Physiology, Faculty of Science And Technology, University of The Basque Country (Upv/Ehu), Leioa, Spain.
4 Ikerbasque, Basque Foundation For Science, Bilbao, Spain.

Contact the author

Keywords

microbial community, botrytis cinerea,  grape composition parameters, next generation sequencing

Citation

Related articles…

Yeast derivatives: an innovative approach to produce Oenococcus oeni under biofilm form?

The malolactic fermentation can occur naturally or be induced by inoculation of selected bacterial strains, most commonly of Oenococcus oeni.

Relation between the environmental factors of the terroir system and flavan-3-ol composition of grape berry seeds and skin at pre-veraison stage and harvest Influence of dedicate viticultural management

Quantity and quality of flavonoïds in grape berries are important parts of their global quality. Several studies had shown that tannins are responsible for some major flavour properties of red wines such as colour, bitterness and astringency. Nevertheless, their synthesis and properties are still misunderstood. Some studies had suggested that the tannic pool was set before veraison. Thus, the comprehension of the relations between environment and setting of this tannic pool, up to the harvest, is not sufficient.

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region.

Caratterizzazione vitivinicola delle “Terre del Piacenziano” ricomprese nella zona D.O.C. “colli piacentini” attraverso l’analisi sensoriale dei vini prodotti

I territori della Riserva Geologica del Piacenziano sono parte del pedeappennino piacentino e sono noti per essere la culla del Pliocene, quel periodo di storia della Terra compreso tra 5.3 e 1.8 milioni di anni fa. Gli strati argillosi e sabbiosi riccamente fossiliferi qui presenti sono da sempre oggetto di studi geo-paleontologici tant’è che il Pliocene medio (3.6-2.6 milioni di anni fa) è internazionalmente noto come Piacenziano. Le analisi sensoriali strutturate dei vini qui prodotti hanno evidenziato, soprattutto per il vino Monterosso, le positive peculiarità dei loro caratteri sensoriali e descritto gli scostamenti significativi del loro profilo sensoriale rispetto agli altri vini presi a riferimento.

Cytochrome P450 CYP71BE5 from grapevine (Vitis vinifera) catalyzes the formation of the spicy aroma compound, (-)-rotundone

(-)-Rotundone, an oxygenated sesquiterpene, is a potent odorant molecule with a characteristic spicy aroma existing in various plants including grapes1. It is considered as a significant compound notably in wines and grapes because of its low sensory threshold (16 ng L-1 in red wine, 8 ng L-1 in water) and aroma properties. (-)-Rotundone was first identified in red wine made from the grape cultivar Syrah (regionally called Shiraz) in Australia1, and then it was found in several grape varieties such as Duras, Grüner Veltliner, Schioppettino and Vespolina from Europe2, 3. Several environmental factors affecting the accumulation of (-)-Rotundone during the grape maturation, were reported such as ambient temperature4, soil properties and topography5, soil moisture from irrigation and light exposure in the bunch zone by leaf removal2.