Macrowine 2021
IVES 9 IVES Conference Series 9 Impact and comprehension of nitrogen and lipid nutrition on the production of fermentative aromas with different S. Cerevisiae yeasts used for spirits

Impact and comprehension of nitrogen and lipid nutrition on the production of fermentative aromas with different S. Cerevisiae yeasts used for spirits

Abstract

In the Cognac appellation, the production of white wines is almost exclusively dedicated to elaborate Charentaise eaux-de-vie. In this sense, the quality of Cognac eaux-de-vie intrinsically depends on the quality of the base wines subjected to the distillation stage. In this context, the production of these base wines differs from those of classic white wines to release particular organoleptic properties during the distillation stage. Thus, the settling stage is one of the stages that most illustrates the identity of Cognac wines. The freshly pressed white grape juice is placed in a settling tank but without the presence of pectolytic enzymes, without sulfiting and for a relatively short period of time, contrary to conventional oenological practices. Under these operating conditions, Cognac musts reach very high turbidities in the order of 500 to 2000 NTU against 150 to 200 NTU in conventional oenology. These Charentais musts, rich in solid particles and therefore in lipids [1], allow to guarantee an organoleptic quality that is both delicate and full of character for future eaux-de-vie. Associated with lipids, nitrogen is a nutrient with a major role in alcoholic fermentation [2] that will also influence the aromatic profile of wines [3] intended for distillation. To understand the impact of these main nutrients on the desired organoleptic quality of Cognac, we studied their influence under natural fermentation conditions with three strains of S. cerevisiae commonly used for the Cognac appellation. To understand the influence of each nutrient and their interaction, an experimental plan called “Central Composite Design” (CCD) was developed. The CCD allows to model the aroma productions from the fermentation conditions. Fermentations were carried out with natural ugni blanc must at 23°C. Assimilable nitrogen concentrations ranged from 115 to 285 mg/L and turbidity from 500 to 2700 NTU. Finally, a statistical analysis of covariance (ANCOVA) was also performed to evaluate the strain effect. The main results showed that lipids and assimilable nitrogen have a significant impact on the aromatic quality of Cognac wines. Indeed, high lipids concentrations favor the production of organic acids but inhibit the synthesis of esters. The metabolism of the 3 yeast strains reacts in the same way to changes in nitrogen and lipid nutrition. However, each strain keeps its own aromatic profile whatever the fermentation conditions. This study made it possible to study and model the impact and interaction of two essential nutrients for alcoholic fermentation on the metabolism of yeast in natural conditions with excess lipids. In addition, it should be noted that, even if each strain of the Cognac appellation has its aroma properties, all strains respond in the same way to the variations of nitrogen and lipid nutrition.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Charlie Guittin, Faïza, Montpellier Isabelle, Jean-Marie, Jean-Roch, SANCHEZ

UMR SPO, INRAE of Montpellier, MACNA, UMR SPO, INRAE Montpellier, , UMR MISTEA, INRAE Montpellier, SABLAYROLLES, UMR SPO, INRAE Montpellier Xavier, POITOU, Hennessy, Cognac, MOURET, UMR SPO, INRAE Montpellier Vincent, FARINES, UMR SPO, INRAE Montpellier

Contact the author

Keywords

cognac, nitrogen, lipids, centered composite design, alcoholic fermentation, Saccharomyces cerevisiae, metabolism, aromas

Citation

Related articles…

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

VINIoT – Precision viticulture service

The project VINIoT pursues the creation of a new technological vineyard monitoring service, which will allow companies in the wine sector in the SUDOE space to monitor plantations in real time and remotely at various levels of precision. The system is based on spectral images and an IoT architecture that allows assessing parameters of interest viticulture and the collection of data at a precise scale (level of grape, plant, plot or vineyard) will be designed. In France, three subjects were specifically developed: evaluation of maturity, of water stress, and detection of flavescence dorée. For the evaluation of maturity, it has been decided first to work at the berry scale in the laboratory, then at the bunch scale and finally in the vineyard. The acquisition of the spectral hyperstal image as well as the reference analyzes to measure the maturity, were carried out in the laboratory after harvesting the berries in a maturity monitoring context. This work focuses on a case study to predict sugar content of three different grape varieties: Syrah, Fer Servadou and Mauzac. A robust method called Roboost-PLSR, developed in the framework of this work (Courand et al., 2022), to improve prediction model performance was applied on spectra after the acquirement of hyperspectral images. Regarding the evaluation of water stress, to work with a significant variability in terms of water status, it has been worked first with potted plants under 2 different water regimes. The facilities have allowed the supervision of irrigation and micro-climatic conditions. The regression models on agronomic variables (stomatal conductance, water potential, …) are studied. To detect flavescence dorée, the experimental plan has consisted of work at leaf scale in the laboratory first, and then in the field. To detect the disease from hyper-spectral imaging, a combination of multivariate curve resolution-alternating least squares (MCR-ALS) and factorial discriminant analysis (FDA) was proposed. This strategy proved the potential towards the discrimination of healthy and infected leaves by flavescence dorée based on the use of hyperspectral images (Mas Garcia et al., 2021).

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

Amino nitrogen content in grapes: the impact of crop limitation

As an essential element for grapevine development and yield, nitrogen is also involved in the winemaking process and largely affects wine composition. Grape must amino nitrogen deficiency affects the alcoholic fermentation kinetics and alters the development of wine aroma precursors. It is therefore essential to control and optimize nitrogen use efficiency by the plant to guarantee suitable grape nitrogen composition at harvest. Understanding the impact of environmental conditions and cultural practices on the plant nitrogen metabolism would allow us to better orientate our technical choices with the objective of quality and sustainability (less inputs, higher efficiency). This trial focuses on the impact of crop limitation – that is a common practice in European viticulture – on nitrogen distribution in the plant and particularly on grape nitrogen composition. A wide gradient of crop load was set up in a homogeneous plot of Chasselas (Vitis vinifera) in the experimental vineyard of Agroscope, Switzerland. Dry weight and nitrogen dynamics were monitored in the roots, trunk, canopy and grapes, during two consecutive years, using a 15N-labeling method. Grape amino nitrogen content was assessed in both years, at veraison and at harvest. The close relationship between fruits and roots in the maintenance of plant nitrogen balance was highlighted. Interestingly, grape nitrogen concentration remained unchanged regardless of crop load to the detriment of the growth and nitrogen content of the roots. Meanwhile, the size and the nitrogen concentration of the canopy were not affected. Leaf gas exchange rates were reduced in response to lower yield conditions, reducing carbon and nitrogen assimilation and increasing intrinsic water use efficiency. The must amino nitrogen profiles could be discriminated as a function of crop load. These findings demonstrate the impact of plant balance on grape nitrogen composition and contribute to the improvement of predictive models and sustainable cultural practices in perennial crops.

The combined effects of climate, soils, and deficit irrigation on yield and quality of Touriga Nacional under high atmospheric demand in the Douro Region

Global warming is one of the biggest environmental, social and economic threats in several viticultural regions. In the Douro Valley, changes are expected in the coming years, namely an increase in temperature and a decrease in precipitation. These changes are likely to have consequences for the production and quality of wine.
The aim of this study was to explore the effects of different soil characteristics combined with several deficit irrigation strategies, managed throughout ETc references and predawn leaf water potentials thresholds, on physiology, yield, and qualitative attributes on the Touriga Nacional variety under years of mild to severe water and heat stress.
The studies were conducted over seven years (2015 to 2021) in two plots of a commercial vineyard located at Quinta do Ataíde (Symington Family Estates) planted in 2011 and 2014 at 170 meters elevation, growing under three water regimes: non-irrigated (NI) and two deficit irrigation strategies (30% and 60% ETc) assessed weekly by Ψpd. The site has an annual rainfall below 500 mm, with high atmospheric demand. Climate data was collected from a weather station, located on site. Berry ripening was followed weekly for fruit analysis. At harvest, yield, vigour and pruning weight per vine were determined from 90 vines by treatment. Each season at veraison the NDVI Index was accessed by a drone. The soils physic-chemistry in the experimental blocs were analysed and grouped by SWHC. Delta C-13 analyses were also performed per treatment in two years.Irrigation had a positive effect on yield per vine, mostly due to an increase in berry and cluster weight, and fertility index through the years. A significant increase in sugar content, colour and phenols was observed with deficit irrigation in some years, but vine vigour related to soil characteristics had by far the greatest impact on quality.