Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of plant fining agents in the must flotation process. Functional characterization

Effect of plant fining agents in the must flotation process. Functional characterization

Abstract

Flotation is one of the most used processes for clarifying white grape must after the pressing process. To date, gelatine is the more used fining agent, its action being improved when combined with bentonite and silica sol. However, in recent years, there is a growing commercial interest in replacing this animal origin protein with plant proteins, due, on the one hand, to the problems associated with allergies and, on the other hand, also thinking in the vegan wine consumers. However the efficiency of plant proteins as floculating agents are lower than gelatine and varies among them, the reason behind the different behaviour being unknown (Marchal et al., 2003; Gambuti et al., 2016; Petinelli et al., 2020). The objective of this work was to compare the flocculating efficiency of a commercial gelatine, a pure pea protein and the same pea protein chemically modified and to relate this efficiency to their amino acid composition and protein functions.The determination of the efficiency was carried out by measuring the percentage of clean must after 1 h from flotation beginning and the measuring of the absorbance at 440 nm to control the browning, while the content of amino acids and peptides was carried out by HPLC-MS. The UNIPROT database was used to obtain the protein functions associated with the peptidesThe results showed that the modified pea protein showed very similar effciency as flotation agent to that of the commercial gelatine and higher than the pure pea protein. The important structural modification made to the pea protein made it more reactive, probably due to a higher exposure of its structure and the apolar and positively charged amino acids, and to the appearance of peptides with protein functions of binding to carbohydrates and proteins, which are also present in animal protein. With these results it can be concluded that pure vegetal proteins may not have sufficientt functional properties to behave as good flocculating agents, although certain chemical modification in their structure may further simulate the behaviour shown by animal protein.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Ana Belén Bautista-Ortín, Sonia, Albendea-Roa,  Jurado

University of Murcia,Bermúdez-Galvez, (University of Murcia) Gómez-Plaza, Encarna (University of Murcia), Mar (Agrovin S.A.), Ricardo (Agrovin, S.A.)

Contact the author

Keywords

finning, amioacids, proteins, flotation, white wine

Citation

Related articles…

Winter physiology in a warmer world: Cold hardiness and deacclimation sensitivity drive variation in spring phenology

As the climate warms, the focus of concern in viticulture often turns to how higher temperatures may shift growing regions, change the character of AVAs, and alter fruit quality. However, climate warming is increasing most quickly during the winter dormancy cycle, a critical and often underappreciated portion of the grapevine life cycle. In response to decreasing temperatures and decreasing daylength, grapes initiate a series of physiological changes to enter dormancy, acquire freeze resistance, and time spring phenology such that the growing season begins after threat of frost.

Integrated approach to grape stalks valorization: sustainable recovery of bioactive compounds and biofuel production

Grape stalks are a byproduct of the winemaking process and represent a valuable and inexpensive source of bioactive compounds. While their direct use in whole bunch fermentation is known, the majority of grape stalks are discarded, posing environmental and economic challenges.

Optimizing the use of bentonite for better control of haze formation In white and rosé wines

In winemaking, the appearance of turbidity in white and wine is a serious visual defect, which lowers significantly its commercial value. A major cause of the formation of turbidity in wine is attributed to the presence of temperature-sensitive proteins.

IMPACT OF ABIOTIC AND BIOTIC FACTORS ON BIOADHESION PROPERTIES OF BRETTANOMYCES BRUXELLENSIS

Brettanomyces bruxellensis is an ubiquitous yeast associated with different fermentation media such as beer and kombucha, where its presence is beneficial to bring an aromatic typicity. However, it is a main spoilage yeast in wines, in which it produces volatile phenols responsible for organoleptic deviations causing significant economic losses (Chatonnet et al., 1992). Cellar and winery equipment’s are considered as the first source of contamination, during fermentation and wine ageing process (Connel et al., 2002). Indeed, it is possible to find B. bruxellensis in the air, on walls and floors of the cellars, on small materials, vats and barrels.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.