Macrowine 2021
IVES 9 IVES Conference Series 9 Phenolic and volatile profiles of south tyrolean pinot blanc musts and young wines

Phenolic and volatile profiles of south tyrolean pinot blanc musts and young wines

Abstract

AIM. Assess the impact of different vineyards and winemaking variables on the phenolic and volatile profiles of Pinot Blanc musts and young wines from South Tyrol.

METHODS. Grapes were harvested during September 2019 in 3 vineyards near Ora (Italy) at 450 m (MM), 550 m (K) and 800 m (V) a.s.l. Six different types of Pinot Blanc musts and young wines were studied in 3 replicates. Study A – 3 different vineyards (MM_C, K_C, V_C), but same winemaking; Study B – same vineyard (V), but 3 different vinifications: i) grapes were frozen before crushing (V_F); ii) same as V_F, but co-inoculation yeast/malolactic bacteria (V_F_ML); iii) no grape freezing, but co-inoculation yeast/malolactic bacteria (V_ML). Phenolics were analysed by HPLC-DAD and HPLC-QqQ-MS, while volatiles were investigated by SPME-HS-GCxGC-ToF-MS. Standard oenological parameters were measured using a multi-parametric analyser, alcohol distillation, pH-meter and chemical titration. The data were statistically processed with ANOVA and Principal Component Analysis (PCA).

RESULTS. Upon a dataset of 27 phenolic compounds identified in musts, a good separation among samples was achieved using PCA. The musts produced without pre-fermentative grape freezing had significantly higher amounts of catechin, gallocatechin and astilbin. Besides, the musts from the same vineyard, but with frozen grapes showed higher concentrations of ethanol, glucose-fructose, malic acid, and lower concentration of tartaric acid. 46 phenolic compounds were identified in wines. The PCA separated well the samples of Study A: caftaric acid showed the most significant difference as well as the highest relative abundance. The PCA showed that the phenolic profile of the wines of Study B (V_C, V_F, V_F_ML, V_ML) clustered samples based on the pre-fermentative grape freezing. Wines made without frozen grapes were separated due to the higher phenolic concentrations. The volatile profile of wines after 1 month of storage contained 32 compounds. The PCA not only grouped samples according to the grape freezing, but it also showed that wines with no applied grape freezing were well clustered in terms of the presence/absence of malolactic fermentation in their winemaking. V_C samples were described by higher abundances of branched chain alcohols, while samples V_ML – by ethyl and phenylethyl esters.

CONCLUSIONS

The profiles of phenolics and volatiles were good discriminants of South Tyrolean Pinot Blanc wines produced under the same winemaking technology but harvested in different vineyards. In this study, the pre-fermentative grape freezing negatively affected concentrations of phenolics. The literature shows that freezing positively enhances contents only of anthocyanins and flavanol glucosides, while it negatively affects contents of phenolic acids and flavanols, that are main phenolic compound in white wines.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Vakare Merkyte

1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy,Simone POGGESI, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Edoardo LONGO, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Fabian STENICO, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Giulia WINDISCH, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Emanuele BOSELLI, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy

Contact the author

Keywords

pinot blanc; white wine; phenolic profile; volatile profile; grape freezing; malolactic fermentation; chemical markers; vinification practices

Citation

Related articles…

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.

Late season canopy management practices to reduce sugar loading and improve color profile of Cabernet-Sauvignon grapes and wines in the high irradiance and hot conditions of California Central Valley

Global warming is accelerating grape ripening, leading to unbalanced wines from fruit with high sugar content but poor aroma and colour development. Reducing the size of the photosynthetic apparatus after veraison has been shown to delay technological ripeness in cool climates, but methods have not been tested in areas with high irradiance and temperature where fruit exposure could have disastrous effects on berry composition. In this Cabernet-Sauvignon trial, we compared the application of an antitranspirant (pinolene), to severe canopy topping and above bunch zone leaf removal, all performed at mid-ripening, with an untouched control. We monitored the vines weekly by measuring stem water potential, gas exchange, fruit zone light exposure. We sampled berries to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, measured carbon isotope discrimination, rated sunburn on clusters, and produced experimental wines. We submitted harvest samples to metabolomic profiling through PFP-Q Exactive MS/MS and wines to sensory analysis. Application of the antitranspirant significantly reduced stomatal conductance and assimilation rate but did not affect the stem water potential. Inversely, leaf removal and topping increased water potential but did not affect leaf gas exchange. The late topping was the only treatment able to decrease sugar content (up to 2Bx), increase titratable acidity and pH, and improve anthocyanin content because of lower degradation of di-hydroxylated forms. Late leaf removal above the bunch zone increased lightning conditions in the canopy and produced the most significant damage on fruits. Yield components were not affected. This work suggests that late-season canopy management can effectively control ripening speeds and improve grapes and wines. Still, the effect on grape exposure in a critical time must be well balanced to avoid problems with the appropriate technique.

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Short-term relationships between climate and grapevine trunk diseases in southern French vineyards

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

The concept of terroir: what place for microbiota?

Microbes play key roles on crop nutrient availability via biogeochemical cycles, rhizosphere interactions with roots as well as on plant growth and health. Recent advances in technologies, such as High Throughput Sequencing Techniques, allowed to gain deeper insight on the structure of bacterial and fungal communities associated with soil, rhizosphere and plant phyllosphere. Over the past 10 years, numerous scientific studies have been carried out on the microbial component of the vineyard. Whether the soil or grape compartments have been taken into account, many studies agree on the evidence of regional delineations of microbial communities, that may contribute to regional wine characteristics and typicity. Some authors proposed the term “microbial terroir” including “yeast terroir” for grapes to describe the connection between microbial biogeography and regional wine characteristics. Many factors are involved in terroir including climate, soil, cultivar and human practices as well as their interactions. Studies considering “microbial terroir” greatly contributed to improve our knowledge on factors that shape the vineyard microbial structure and diversity. However, the potential impact of “microbial terroir” on wine composition has yet not received strong scientific evidence and many questions remain to be addressed, related to the functional characterization of the microbial community and its impact on plant physiology and grape composition, the origins and interannual stability of vineyard microbiota, as well as their impact on wine sensorial attributes. The presentation will give an overview on the role of microbiota as a terroir component and will highlight future perspectives and challenges on this key subject for the wine industry.