Macrowine 2021
IVES 9 IVES Conference Series 9 Phenolic and volatile profiles of south tyrolean pinot blanc musts and young wines

Phenolic and volatile profiles of south tyrolean pinot blanc musts and young wines

Abstract

AIM. Assess the impact of different vineyards and winemaking variables on the phenolic and volatile profiles of Pinot Blanc musts and young wines from South Tyrol.

METHODS. Grapes were harvested during September 2019 in 3 vineyards near Ora (Italy) at 450 m (MM), 550 m (K) and 800 m (V) a.s.l. Six different types of Pinot Blanc musts and young wines were studied in 3 replicates. Study A – 3 different vineyards (MM_C, K_C, V_C), but same winemaking; Study B – same vineyard (V), but 3 different vinifications: i) grapes were frozen before crushing (V_F); ii) same as V_F, but co-inoculation yeast/malolactic bacteria (V_F_ML); iii) no grape freezing, but co-inoculation yeast/malolactic bacteria (V_ML). Phenolics were analysed by HPLC-DAD and HPLC-QqQ-MS, while volatiles were investigated by SPME-HS-GCxGC-ToF-MS. Standard oenological parameters were measured using a multi-parametric analyser, alcohol distillation, pH-meter and chemical titration. The data were statistically processed with ANOVA and Principal Component Analysis (PCA).

RESULTS. Upon a dataset of 27 phenolic compounds identified in musts, a good separation among samples was achieved using PCA. The musts produced without pre-fermentative grape freezing had significantly higher amounts of catechin, gallocatechin and astilbin. Besides, the musts from the same vineyard, but with frozen grapes showed higher concentrations of ethanol, glucose-fructose, malic acid, and lower concentration of tartaric acid. 46 phenolic compounds were identified in wines. The PCA separated well the samples of Study A: caftaric acid showed the most significant difference as well as the highest relative abundance. The PCA showed that the phenolic profile of the wines of Study B (V_C, V_F, V_F_ML, V_ML) clustered samples based on the pre-fermentative grape freezing. Wines made without frozen grapes were separated due to the higher phenolic concentrations. The volatile profile of wines after 1 month of storage contained 32 compounds. The PCA not only grouped samples according to the grape freezing, but it also showed that wines with no applied grape freezing were well clustered in terms of the presence/absence of malolactic fermentation in their winemaking. V_C samples were described by higher abundances of branched chain alcohols, while samples V_ML – by ethyl and phenylethyl esters.

CONCLUSIONS

The profiles of phenolics and volatiles were good discriminants of South Tyrolean Pinot Blanc wines produced under the same winemaking technology but harvested in different vineyards. In this study, the pre-fermentative grape freezing negatively affected concentrations of phenolics. The literature shows that freezing positively enhances contents only of anthocyanins and flavanol glucosides, while it negatively affects contents of phenolic acids and flavanols, that are main phenolic compound in white wines.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Vakare Merkyte

1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy,Simone POGGESI, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Edoardo LONGO, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Fabian STENICO, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Giulia WINDISCH, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy Emanuele BOSELLI, 1. Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy; 2. Oenolab, NOI Techpark South Tyrol, Via A. Volta 13B, 39100 Bozen-Bolzano, Italy

Contact the author

Keywords

pinot blanc; white wine; phenolic profile; volatile profile; grape freezing; malolactic fermentation; chemical markers; vinification practices

Citation

Related articles…

Making sense of available information for climate change adaptation and building resilience into wine production systems across the world

Effects of climate change on viticulture systems and winemaking processes are being felt across the world. The IPCC 6thAssessment Report concluded widespread and rapid changes have occurred, the scale of recent changes being unprecedented over many centuries to many thousands of years. These changes will continue under all emission scenarios considered, including increases in frequency and intensity of hot extremes, heatwaves, heavy precipitation and droughts. Wine companies need tools and models allowing to peer into the future and identify the moment for intervention and measures for mitigation and/or avoidance. Previously, we presented conceptual guidelines for a 5-stage framework for defining adaptation strategies for wine businesses. That framework allows for direct comparison of different solutions to mitigate perceived climate change risks. Recent global climatic evolution and multiple reports of severe events since then (smoke taint, heatwave and droughts, frost, hail and floods, rising sea levels) imply urgency in providing effective tools to tackle the multiple perceived risks. A coordinated drive towards a higher level of resilience is therefore required. Recent publications such as the Australian Wine Future Climate Atlas and results from projects such as H2020 MED-GOLD inform on expected climate change impacts to the wine sector, foreseeing the climate to expect at regional and vineyard scale in coming decades. We present examples of practical application of the Climate Change Adaptation Framework (CCAF) to impacts affecting wine production in two wine regions: Barossa (Australia) and Douro (Portugal). We demonstrate feasibility of the framework for climate adaptation from available data and tools to estimate historical climate-induced profitability loss, to project it in the future and to identify critical moments when disruptions may occur if timely measures are not implemented. Finally, we discuss adaptation measures and respective timeframes for successful mitigation of disruptive risk while enhancing resilience of wine systems.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Elucidating vineyard site contributions to key sensory molecules: Identification of correlations between elemental composition and volatile aroma profile of site-specific Pinot noir wines

The reproducibility of elemental profile in wines produced across multiple vintages has been previously reported using grapes from a single scion clone of Vitis vinifera L. cv. Pinot noir. The grapevines were grown on fourteen different vineyard sites, from Oregon to southern California in the U.S.A., which span distances from approximately hundreds of meters to 1450 km, while elevations range from near sea level to nearly 500 m. In addition, sensorial (i.e. aroma, taste, and mouthfeel) and chemical (i.e. polyphenolic and volatile) differences across the different vineyard sites have also been observed among these wines at two aging time points. While strong evidence exists to support that grapes grown in different regions can produce wines with unique chemical and sensorial profiles, even when a single clone is used, the understanding of growing site characteristics that result in this reproducible differentiation continues to emerge. One hypothesis is that the elemental profile that a vineyard site imparts to the grape berries and the resulting wine is an important contributor to this differentiation in chemistry and sensory of wines. For example, various classes of enzymes that catalyze the formation of key aroma compounds or their precursors require specific metals. In this work, we begin to report correlations between elemental and volatile aroma profiles of site-specific Pinot noir wines, made under standardized winemaking conditions, that have been previously shown to be distinguished separately by these chemical analyses.

Assessing the climate change vulnerability of European winegrowing regions by combining exposure, sensitivity and adaptive capacity indicators

Winegrowing regions recognized as protected designations of origin (PDOs) are closely tied to well defined geographic locations with a specific set of pedoclimatic attributes and strictly regulated by legal specifications. However, climate change is increasingly threatening these regions by changing local conditions and altering winegrowing processes. The vulnerability to these changes is largely heterogenous across different winegrowing regions because it is determined by individual characteristics of each region, including the capacity to adapt to new climatic conditions and the sensitivity to climate change, which depend not only on natural, but also socioeconomic and legal factors. Accurate vulnerability assessments therefore need to combine information about adaptive capacity and climate change sensitivity with projected exposure to new climatic conditions. However, most existing studies focus on specific impacts neglecting important interactions between the different factors that determine climate change vulnerability. Here, we present the first comprehensive vulnerability assessment of European wine PDOs that spatially combines multiple indicators of adaptive capacity and climate change sensitivity with high-resolution climate projections. We found that the climate change vulnerability of PDO areas largely depends on the complex interactions between physical and socioeconomic factors. Homogenous topographic conditions and a narrow varietal spectrum increase climate change vulnerability, while the skills and education of farmers, together with a good economic situation, decrease their vulnerability. Assessments of climate change consequences therefore need to consider multiple variables as well as their interrelations to provide a comprehensive understanding of the expected impacts of climate change on European PDOs. Our results provide the first vulnerability assessment for European winegrowing regions at high spatiotemporal resolution that includes multiple factors related to climate exposure, sensitivity, and adaptive capacity on the level of single winegrowing regions. They will therefore help to identify hot spots of climate change vulnerability among European PDOs and efficiently direct adaptation strategies.

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).