Macrowine 2021
IVES 9 IVES Conference Series 9 Exploring the resistance of non-Saccharomyces wine yeasts to dehydration-rehydration processes

Exploring the resistance of non-Saccharomyces wine yeasts to dehydration-rehydration processes

Abstract

AIM: The use of non-Saccharomyces (NS) yeasts in multi-starter fermentations with S. cerevisiae is a trend in the wine industry, but the number of strains commercially available in a powder formulation, such as active dry yeasts (ADY), is still limited. Considering that NS strains are bioresources which could boost innovation in the wine sector, efficient production of powder formulations is of utmost importance to valorise bioresources outside the laboratory environment.

METHODS: Three strains with high oenological potential, selected in previous studies, namely Lachancea thermotolerans DBT027, Metschnikowia sp. DBT012 and Starmerella bacillaris DBT045 were grown in a sterilized medium mainly constituted by grape juice with yeast assimilable nitrogen integration. Early stationary growth phase cultures were collected and suspended in 1% yeast extract, for powder production. The three prototype batches were rehydrated in tap water, to mimic winery conditions, for 15 minutes at four temperatures (20, 25, 30 and 35 ºC). Survival rates of yeasts were assessed before and after rehydration, and after storage at -20 ºC. The same powder NS yeasts were also in multi-starter fermentations in winery and their persistence was monitored.

RESULTS: The selected NS yeast strains were able to grow in the production medium with similar yields, and the powder formulation retained elevated cell viability (around 109-1010 cells/g). The optimal rehydration temperature was different for the three strains: Metschnikowia sp. DBT012 and L. thermotolerans DBT027 maintained higher survival at 35 ºC, while S. bacillaris DBT045 at 20 ºC, as could be expected considering the phenotypic characteristics of the species. Technological performances of the tested powder formulations were satisfactory, especially for DBT012, which was able to grow in grape must after inoculation and dominated over the indigenous microbiota.

CONCLUSIONS

The applied protocol for producing NS yeasts in the powder formulation was successful, as they retained high viability during rehydration and suitable activity when inoculated in must. Powder formulation guaranteed the conservation of the same batch for several comparative tests, bringing out their positive contribution to innovative winemaking biotechnologies. The ability to withstand a larger scale process of biomass production is essential for the exploitation of suitable non-Saccharomyces yeasts selected among a collection of yeast strains with proven oenological potential at laboratory scale. ACKNOWLEDGMENTS: Research developed in the framework of POR FESR 2014-2020 Regione del Veneto, project VIT-VIVE

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Giovanna Felis 

Department of Biotechnology, University of Verona, Italy,Renato Leal BINATI, Dept. Biotechnology, University of Verona, Italy Eleonora TROIANO, Dept. Biotechnology, University of Verona, Italy Sandra TORRIANI, Dept. Biotechnology, University of Verona, Italy Marta TEBALDI, Microbion S.r.L. Italy Alessandro RONCADOR, Microbion S.r.L. Italy Fabio FRACCHETTI, Microbion S.r.L. Italy

Contact the author

Keywords

starter cultures, biomass production, dehydration, active dry yeasts

Citation

Related articles…

Differential responses of red and white grape cultivars trained to a single trellis system – the VSP

Commercial grape production relies on training grapevine cultivars onto a variety of trellis systems. Training allows for well-lit leaves and clusters, maximizing fruit quality in addition to facilitating cultivation, harvesting, and diseases control. Although grapevines can be trained onto an infinite variety of trellis systems, most red and white cultivars are trained to the standard VSP (Vertical Shoot Positioning) system. However, red and white cultivars respond differently to VSP in fruit composition and growth characteristics, which are yet to be fully understood. Therefore, the objective of this study was to examine the influence of the VSP trellis system on fruit composition of three red, Cabernet Sauvignon, Merlot and Syrah, and three white, Chardonnay, Riesling, and Gewurztraminer cultivars grown under uniform growing conditions in the same vineyard. All cultivars were monitored for maturity and harvested at their physiologically maximum possible sugar concentration to compare various fruit quality attributes such as Brix, pH, TA, malic and tartaric acids, glucose and fructose, potassium, YAN, and phenolic compounds including total anthocyanins, anthocyanin profile, and tannins. A distinct pattern in fruit composition was observed in each cultivar. In regards to growth characteristics, Syrah grew vigorously with the highest cluster weight. Although all cultivars developed pyriform seeds, the seed size and weight varied among all cultivars. Also varied were mesocarp cell viability, brush morphology, and cane structure. This knowledge of the canopy architectural characteristics assessed by the widely employed fruit compositional attributes and growth characteristics will aid the growers in better management of the vines in varied situations.

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

A spatial explicit inventory of EU wine protected designation of origin to support decision making in a changing climate

Winemaking areas recognized as protected designations of origin (PDOs) shape important economic, environmental and cultural values that are tied to closely defined geographic locations. To preserve wine products and wine-growing practices adopted in different PDOs these areas are strictly regulated by legal specifications. However, quality viticulture is increasingly under pressure from climate change, which is altering the local conditions of many winegrowing areas. Therefore, maintaining traditional wine products will require the adoption of tailored adaptation strategies, including possible changes in the legal regulation of protected wines. To this end, it is necessary to have a comprehensive knowledge on PDOs including their extension, products and allowed practices. While there have been efforts to build databases that summarize the characteristics for individual wine PDO areas and to quantify the related effects of climate change, much information is still included only in the official documentation of the EU geographical indication register and has never been collected in a comprehensive manner. With this study we aim at filling this gap by building a spatial inventory of European wine PDOs that supports decision making in viticulture in the context of climate change. To map and characterize European wine PDOs, we analysed their legal documents and extracted relevant information useful for climate change adaptation. The output consists of a comprehensive geographical dataset that identifies the boundaries of all 1200 European wine PDOs at unprecedented spatial resolution and includes a set of legally binding regulations, such as authorized vine varieties, maximum yields and planting density. The inventory will allow researchers to analyse the impacts of climate change on European wine PDOs and support decision makers in developing tailored adaptation strategies. This includes, among others, the evaluation of new vineyard site selection, the expansion of cultivated varieties or the authorization of irrigation in vineyards.

Sustainable fertilisation of the vineyard in Galicia (Spain)

Excessive fertilization of the vineyard leads to low quality grapes, increased costs and a negative impact on the environment. In order to establish an integrated management system aimed at a sustainable fertilization of the vineyards, nutritional reference levels were established. For this purpose, 30 representative vineyards of the Albariño variety were studied, in which soil and petiole analyses were carried out for two years and grape yield and quality at harvest were measured. In both years of study, soil pH, calcium, sodium and cation exchange capacity were positively correlated with calcium content and negatively correlated with manganese in grapes. Irrigated vineyards had higher levels of aluminium in soil and lower levels of calcium in petiole. Climatic conditions were very different in the years of the study. The year 2019 was colder than usual, in 2020 there was a marked water stress with high summer temperatures. This resulted in medium-high acidity in grapes in 2019 and low acidity in 2020, with sugar levels being similar both years. A very marked decrease in must amino nitrogen was observed in 2020, with ammonia nitrogen remaining stable. The correlation of acidity and sugar values in grapes with soil and petiole analysis data made it possible to establish reference levels for the nutritional diagnosis of the Albariño variety in this region. Based on these results, an easy-to-use TIC application is currently being created for grapegrowers, aimed at improving the sustainability of the vineyard through reasoned fertilization. This study has now been extended to other Galician vine varieties.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)