Insights from selected ion flow tube mass spectrometry (SIFT-MS) and chemometrics applied to the quick discrimination of grapevine varieties

‘Vignoles’ is an aromatic, white-fruited wine grape variety valued by growers and wineries in the Eastern United States. Vignoles is grown in diverse locations in New York, Missouri, Indiana, Ohio, Pennsylvania, Illinois, Nebraska and Michigan. Consumers recognize and value the variety for its special wine quality.

Valpolicella is a famous wine producing region located in the north of Verona close to Garda lake and owes its fame above all to the production of two Protected Designation of Origins (PDOs) withered wines: Amarone and Recioto. Nowadays the production of another PDO, Valpolicella Superiore is gaining more attention by the consumers, increasing the interest of the wineries to improve the quality of this wines

Winegrowers must adapt more and more their viticultural practices in order to evolve toward a sustainable viticulture, to be competitive and to improve both the production methods and the quality and typicalness of wines. In this context, ‘Terroir’ studies in Loire Valley vineyards have allowed to build decision aid maps that can be used directly by growers to adjust their practices.

Field irrigation experiments were performed on young « Nonpareil » almond trees, mature « Bartlett » pear trees and mature « Pinot Noir » grapevines, to determine the relation of a number of alternative measures of plant water status (predawn and midday stem and leaf water potential), to a number of indices of plant physiological activity (leaf conductance, vegetative growth and fruit growth and composition).

Our research aimed to determine the effect and efficiency of foliar application of urea on the phenolic composition of Tempranillo Blanco grapes. The field experiment was carried out in 2019 and 2020 seasons and the plot was located in D.O.Ca Rioja (North of Spain). The vineyard was Vitis vinifera L. Tempranillo Blanco and grafted on Richter-110 rootstock. The treatments were control (C), whose plants were sprayed with water and three doses of urea: plants were sprayed with urea 3 kg N/ha (U3), 6 kg N/ha (U6) and 9 kg N/ha (U9). The applications were performed in two phenological stages, pre-veraison (Pre) and veraison (Ver). Also, each of the treatments was repeated one week later. Control and treatments were performed in triplicate and arranged in a randomised block design. Grapes were harvested at optimum ripening stage. High-performance liquid chromatography was used to analyse the phenolic composition of the grapes. Finally, the results obtained from the analytical determinations – flavonols, flavanols and non-flavonoid (hydroxybenzoic acids, hydroxycinnamic acids and stilbenes) – were studied statistically by analysis of variance. The results showed that, in 2019, U6-Pre and U9-Pre treatments increased the hydroxybenzoic acid content in grapes, and also all foliar treatments applied at Pre enhanced the stilbene concentration. Moreover, U3-Ver was the only treatment that rose flavonol and stilbene contents in the Tempranillo Blanco grapes. In 2020, all treatments applied at Pre enhanced the flavonol concentration in grapes. Furthermore, U3-Pre and U9-Pre treatments increased stilbene content in grapes. Nevertheless, the hydroxybenzoic acid content was improved by U6-Ver and U9-Ver and besides, hydroxycinnamic acid concentration in grapes was increased by all treatments applied at Ver. In conclusion, the lower and highest dose of urea (U3 and U9), applied at pre-veraison, were the best treatments to improve the Tempranillo Blanco grape phenolic composition.