Macrowine 2021
IVES 9 IVES Conference Series 9 Red wine oxidation: oxygen consumption kinetics and high resolution uplc-ms analysis

Red wine oxidation: oxygen consumption kinetics and high resolution uplc-ms analysis

Abstract

Oxygen is playing a major role in wine ageing and conservation. Many chemical oxidation reactions occur but they are difficult to follow due to their slow reaction times and the numerous resulting reaction products. There is a need for global and rapid in vitro tests to predict wine oxidation kinetics. First, three different forced oxidation protocols were developed on a “young” (2018) red wine to follow the consumption of oxygen. After oxygen saturation of the wines at 22°C, the red wines were oxidized following 3 different protocols

1 – heat at 60°C

2 –laccase oxidation at 22°C

3 –hydrogen peroxide oxidation at 22°C

The oxygen consumption kinetics were followed by oxo-luminescence oxygen measurements. The oxygen consumption all followed a first order kinetic on the 2018 wine but had different kinetics constants depending on the oxidation protocol. High resolution UPLC-MS was also performed on forced oxidation samples and compared to natural oxidation samples of naturally aged red wines (2014 and 2010 vintages). Specific polyphenols (anthocyanins, flavanols and their derivatives) were impacted in both naturally or artificially aged wines and differed depending on the oxidation protocol. For example, the intensity of some low molecular weight polyphenols increased both in naturally or artificially heated aged wines ([M+H]+= 287; 289; 291; 303; 317; 319). However, some differences were observed between natural and artificial aging for higher molecular weight polyphenols ([M+H]+= 493; 535; 639)

DOI:

Publication date: September 13, 2021

Issue: Macrowine 2021

Type: Article

Authors

Stacy Deshaies

SPO, Univ Montpellier, INRA, Montpellier SupAgro, Montpellier, France.,Guillaume CAZALS: IBMM, Univ Montpellier, Montpellier, France  Christine ENJALBAL: IBMM, Univ Montpellier, Montpellier, France  François GARCIA :SPO, Univ Montpellier, INRA, Montpellier SupAgro, Montpellier, France. Laetitia MOULS: SPO, Univ Montpellier, INRA, Montpellier SupAgro, Montpellier, France. Cédric SAUCIER: SPO, Univ Montpellier, INRA, Montpellier SupAgro, Montpellier, France.

Contact the author

Keywords

wine; oxidation; polyphenol; syrah; mass spectrometry; oxygen; vintage; markers

Citation

Related articles…

Impact of winemaking processes on wine polysaccharides, improving by qNMR

Today the knowledge in terms of molecular composition of the colloidal matrix is ​​not enough in order to control its stability, according to the number of winemaking and wine stabilization processes. The physico-chemical processes during the winemaking change the composition and quantity of wine macromolecules. The goal today is to determine which analytical techniques will allow to discriminate these winemaking processes in order to better understand their impact on colloidal matrix stability as well as which molecules are responsible for its instabilities. METHODS: Wines obtained after conventional winemaking were subjected to different fining and chemical stabilization treatments. Different methods were used to investigate the wine macromolecular composition and stability after chemical stabilization, including quantitative and qualitative analyzes of total soluble polysaccharides by extraction under acidified ethanol, and by size exclusion separation as well as qNMR metabolomics. RESULTS: Observation of a slight difference at the quantitative level using classical analysis between the winemaking processes was observed as well as a strong discrimination by qNMR metabolomics.

Reducing chemical use in vineyards. Evidence from the analysis of a national demonstration Network

High quantities of chemicals are applied in the vineyard for pest and disease control. Transition towards low pesticide viticulture is a key issue to improve sustainability. Winegrowers have to gradually change their practices to engage in this transition. This work aims at analysing the pesticide use evolution during transition towards low pesticide vineyards and identify some management options mobilized by winegrowers. To understand the diversity of pathways taken towards agroecological transition, we characterized different types of pesticide use evolution.

Harnessing whole genome sequencing data to predict protein structure and function variation in grapevine

Grapevine (Vitis vinifera) is amongst the world’s most cultivated fruit crops, and of global and economic significance, producing a wide variety of grape-derived products, including wine, and table grapes. The genus Vitis, encompassing approximately 70 naturally occurring inter-fertile species, exhibits extensive genetic and phenotypic diversity, highlighted by the global cultivation of thousands of predominantly Vitis vinifera cultivars. Despite the importance of harnessing its naturally occurring genetic diversity to pursue traits of interest, especially considering the continued and growing demand for sustainable high-quality grape production, the systematic characterization of available functional genetic variants remains limited.

Climatic zoning of viticultural production periods over the year in the tropical zone: application of the methodology of the Géoviticulture MCC system

L’objectif de cette recherche est le zonage climatique des périodes viticoles de l’année dans la Vallée du São Francisco, région brésilienne productrice de vins située en climat tropical semi-aride. Dans cette région, la production peut être échelonnée sur tous les mois de l’année.

Effects of the synergy between T. delbrueckii and S. cerevisiae in the winemaking of traditional cultivars from southeastern Italy

The combination of Torulaspora delbrueckii and Saccharomyces cerevisiae in co-inoculation and sequential inoculation in winemaking was investigated as an innovative strategy to increase the aromatic profile of wines like Verdeca and Nero di Troia wines, two traditional varieties from south-eastern Italy (Apulia Region).