Macrowine 2021
IVES 9 IVES Conference Series 9 Influence of the type of flavonol and the presence of mannoproteins in the copigmentation with malvidin 3-O-glucoside

Influence of the type of flavonol and the presence of mannoproteins in the copigmentation with malvidin 3-O-glucoside

Abstract

AIM: To study the copigmentation between different wine flavonols (myricetin, quercetin, kaempferol, isorhamnetin and syringetin 3-O-glucosides) and malvidin 3-O-glucoside to detect differences in the interactions due to the flavonol type. Considering the existing interactions of anthocyanins and flavonols in wines with mannoproteins1, copigmentation was also studied in the presence of 5 different mannoproteins.

METHODS: 36 model systems were built in wine-like solution in triplicate. One of them contained only the anthocyanin. 10 were anthocyanin-flavonol (AF) or anthocyanin-mannoprotein (AM) binary systems. 25 contained the anthocyanin, one type of flavonol and one type of mannoprotein (AFM). Concentrations used were 0.41mM for the anthocyanin and flavonol and 400 mg/L for the mannoprotein. UV-vis spectra were measured at days 1, 2, 5, 8 and 22, calculating then, CIELAB parameters and copigmentation indexes, such as CCI (% of colour due to copigmentation)2. HPLC-DAD-MSn analyses1 were performed at day 22 to study anthocyanin degradation and the possible and distinct formation of anthocyanin-derived pigments.

RESULTS: In AF binary systems, the occurrence of copigmentation was demonstrated from the CCI values (>23 in all model systems from day 1 to day 22). Copigmentation increased during the first 8 days and then tended to decrease until day 22. Isorhamnetin 3-O-glucoside appeared to be the best copigment (CCI 70) whereas kaempferol 3-O-glucoside caused the lowest CCI values. In AM binary systems, copigmentation was not observed and, for some mannoproteins, even an anticopigmentation effect was detected. In AFM ternary systems, the effect depended both on the flavonol and on the mannoprotein, pointing to different interactions probably related to structural differences. 

CONCLUSIONS

The magnitude of the copigmentation phenomenon between malvidin 3-O-glucoside and different flavonols depend on the structure of the flavonol and can be differently affected by the presence of different types of mannoproteins in the medium.

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Cristina Alcalde Eon

Grupo de Investigación en Polifenoles, University of Salamanca, Salamanca, Spain,María de las Nieves FELIPE-JIMÉNEZ. Grupo de Investigación en Polifenoles, University of Salamanca, Salamanca, Spain Ignacio GARCÍA-ESTÉVEZ. Grupo de Investigación en Polifenoles, University of Salamanca, Salamanca, Spain María Teresa ESCRIBANO-BAILÓN. Grupo de Investigación en Polifenoles, University of Salamanca, Salamanca, Spain

Contact the author

Keywords

anthocyanins, flavonols, copigmentation, mannoproteins, colour stability

Citation

Related articles…

Mechanisms involved in the heating of the environment by the aerodynamic action of a wind machine to protect a vineyard against spring frost

One of the main consequences of global warming is the rise of the mean temperature. Thus, the heat summation by the plants begins sooner in the early spring, and by cumulating growing degree-days, phenological development tends to happen earlier. However, spring frost is still a recurrent phenomenon causing serious damages to buds and therefore, threatening the harvests of the winegrowers. The wind machine is a solution to protect fruit crops against spring frost that is increasingly used. It is composed of a 10-m mast with a blowing fan at its peak. By tapping into the strength of the nocturnal thermal inversion, it sweeps the crop by propelling warm air above to the ground. Thus, stratification is momentarily suppressed. Furthermore, the continuous action of the machine, alone or in synergy, or the addition of a heater allow the bud to be bathed in a warmer environment. Also, the punctual action of the tower’s warm gust reaches the bud directly at each rotation period. All these actions allow the bud to continuously warm up, but with different intensities and over a different period. Although there is evidence of the effectiveness of the wind machines, the thermal transfers involved in those mechanisms raise questions about their true nature. Field measurements based on ultrasonic anemometers and fast responding thermocouples complemented by laboratory measurements on a reduced scale model allow to characterize both the airflow produced by the wind machine and the local temperature in its vicinity. Those experiments were realized in the vineyard of Quincy, in the framework of the SICTAG project. In the future paper, we will detail the aeraulic characterization of the wind machine and the thermal effects resulting from it and we will focus on how the wind machine warms up the local atmosphere and enables to reduce the freezing risk.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Characterization of variety-specific changes in bulk stomatal conductance in response to changes in atmospheric demand and drought stress

In wine growing regions around the world, climate change has the potential to affect vine transpiration and overall vineyard water use due to related changes in atmospheric demand and soil water deficits. Grapevines control their transpiration in response to a changing environment by regulating conductance of water through the soil-plant-atmosphere continuum. Most vineyard water use models currently estimate vine transpiration by applying generic crop coefficients to estimates of reference evapotranspiration, but this does not account for changes in vine conductance associated with water stress, nor differences thought to exist between varieties. The response of bulk stomatal conductance to daily weather variability and seasonal drought stress was studied on Cabernet-Sauvignon, Merlot, Tempranillo, Ugni blanc, and Semillon vines in a non-irrigated vineyard in Bordeaux France. Whole vine sap flow, temperature and humidity in the vine canopy, and net radiation absorbed by the vine canopy were measured on 15-minute intervals from early July through mid-September 2020, together with periodic measurement of leaf area, canopy porosity, and predawn leaf water potential. From this data, bulk stomatal conductance was calculated on 15-minute intervals, and multiple regression analysis was performed to identify key variables and their relative effect on conductance. Attention was focused on addressing multicollinearity and time-dependency in the explanatory variables and developing regression models that were readily interpretable. Variability of vapor pressure deficit over the day, and predawn water potential over the season explained much of the variability in conductance, with relative differences in response coefficients observed across the five varieties. By characterizing this conductance response, the dynamics of vine transpiration can be better parameterized in vineyard water use modeling of current and future climate scenarios.

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.