Macrowine 2021
IVES 9 IVES Conference Series 9 First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

Abstract

An organoleptic defect, called fresh mushrooms off-flavor, appeared in wines and spirits since the 2000’s. Numerous researches demonstrated that octen-3-one, octan-3-ol and octen-3-ol (C8 compounds) were involved in the mushroom off-flavor in wines (Pallotta et al., 1998), (Darriet et al., 2002). Botrytis cinerea and other moldsplayed a role in the metabolism of such molecules directly on the grapes (La Guerche et al., 2006). Moreover, producers couldn’t detect this off-flavor in must but it appeared only in finished wines and the intensity can vary considerably during ageing of wines. Several biogenesis pathways have been proposed to explain the mushroom off-flavor in foods. In the fungus kingdom, the formation of C8 molecules came from the transformation of linoleic acid under the action of several enzymes (Wurzenberger & Grosch, 1984). Glycosidic precursors of octen-3-ol have been identified in several plants such as recently in soybean (Matsui et al., 2018). However, under oenological conditions, no clear mechanism has been established yet. AIM: So, the aim of this work was to (i) identify glycosidic precursors of C8 compounds and (ii) to evaluate the influence of different rots on the glycosylated fractions of different grape varieties. For this purpose, we studied different grades of rot defined by visual intensity (healthy, 1-5%, 10-15% and 20-25% of rots) of grapes and musts of Meunier, Pinot noir and Chardonnay affected by Botrytis cinerea, Powdery mildew and Crustomyces subabruptus. METHODS: From analytical point of view, glycosylated precursors were extracted on C18 cartridges (Lichlorut RP-18, 500 mg),then cleaved by a beta-glycosidase enzyme (Rapidase Revelation Aroma, 40°C, Overnight) and aglycones were analyzed byGC-MS (FS and SIM mode) as reported by Schneider in 2001. RESULTS: For the first time, we identified a glycosylated fraction able to release fresh mushroom aroma (octen-3-one, octen-3-ol, octan-3-ol) in Meunier and Pinot noir musts. Indeed, contaminated musts of Pinot noir treated by beta glucosidase enzyme released three times more octen-3-one than control. In the samples infected with Powdery mildew, we didn’t observe any production of these glycosides. Botrytis cinerea seemed to decrease the level of octen-3-one glycoside(s) as soon as level contamination reached at least 1%. Finally, a Pinot noir must contaminated by Crustomyces subabruptus involved an increase of the glycosylated fraction responsible for the octen-3-one by 31% in comparison with uncontaminated sample. CONCLUSION: In conclusion, the identification of a glycosylated fraction able to release fresh mushroom aroma compounds opens avenue to better understand the source of this specific taint and constitutes the first step to help winemakers to avoid off-flavor.

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Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Léa Delcros 

MHCS, Comité Champagne, Epernay, France,Teddy GODDET, SPO, Univ Montpellier INRAE, Institut agro, Montpellier, France  Sylvie COLLAS, Comité Champagne, Epernay, France  Marion HERVE, MHCS, Epernay, France  Bruno BLONDIN, SPO, Univ Montpellier INRAE, Institut agro, Montpellier, France  Aurélie ROLAND, SPO, Univ Montpellier, INRAE, Institut agro, Montpellier, France

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octen-3-one, octen-3-ol, glycosides, mushroom off-flavor, wine, botrytis cinerea, powdery mildew, crustomyces subabruptus

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Macrowine 2021
IVES 9 IVES Conference Series 9 First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

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Spatiotemporal patterns of chemical attributes in Vitis vinifera L. cv. Cabernet Sauvignon vineyards in Central California

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Wine growing regions have recently faced intense and frequent droughts that have led to substantial economical losses, and the maintenance of grapevine productivity under warmer and drier climate will rely notably on planting drought-resistant cultivars. Given that plant growth and yield depend on water transport efficiency and maintenance of photosynthesis, thus on the preservation of the vascular system integrity during drought, a better understanding of drought-related hydraulic traits that have a significant impact on physiological processes is urgently needed. We have worked towards this end by assessing vulnerability to xylem embolism in 30 grapevine commercial varieties encompassing red and white Vitis vinifera varieties, hybrid varieties characterized by a polygenic resistance for powdery and downy mildew, and commonly used rootstocks. These analyses further allowed a global assessment of wine regions with respect to their varietal diversity and resulting vulnerability to stem embolism. Hybrid cultivars displayed the highest vulnerability to embolism, while rootstocks showed the greatest resistance. Significant variability also arose among Vitis vinifera varieties, with Ψ12 and Ψ50 values ranging from -0.4 to -2.7 MPa and from -1.8 to -3.4 MPa, respectively. Cabernet franc, Chardonnay and Ugni blanc featured among the most vulnerable varieties while Pinot noir, Merlot and Cabernet Sauvignon ranked among the most resistant. In consequence, wine regions bearing a significant proportion of vulnerable varieties, such as Poitou-Charentes, France and Marlborough, New Zealand, turned out to be at greater risk under drought. These results highlight that grapevine varieties may not respond equally to warmer and drier conditions, outlining the importance to consider hydraulic traits associated with plant drought tolerance into breeding programmes and modeling simulations of grapevine yield maintenance under severe drought. They finally represent a step forward to advise the wine industry about which varieties and regions would have the lowest risk of drought-induced mortality under climate change.

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The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
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