Macrowine 2021
IVES 9 IVES Conference Series 9 First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

Abstract

An organoleptic defect, called fresh mushrooms off-flavor, appeared in wines and spirits since the 2000’s. Numerous researches demonstrated that octen-3-one, octan-3-ol and octen-3-ol (C8 compounds) were involved in the mushroom off-flavor in wines (Pallotta et al., 1998), (Darriet et al., 2002). Botrytis cinerea and other moldsplayed a role in the metabolism of such molecules directly on the grapes (La Guerche et al., 2006). Moreover, producers couldn’t detect this off-flavor in must but it appeared only in finished wines and the intensity can vary considerably during ageing of wines. Several biogenesis pathways have been proposed to explain the mushroom off-flavor in foods. In the fungus kingdom, the formation of C8 molecules came from the transformation of linoleic acid under the action of several enzymes (Wurzenberger & Grosch, 1984). Glycosidic precursors of octen-3-ol have been identified in several plants such as recently in soybean (Matsui et al., 2018). However, under oenological conditions, no clear mechanism has been established yet. AIM: So, the aim of this work was to (i) identify glycosidic precursors of C8 compounds and (ii) to evaluate the influence of different rots on the glycosylated fractions of different grape varieties. For this purpose, we studied different grades of rot defined by visual intensity (healthy, 1-5%, 10-15% and 20-25% of rots) of grapes and musts of Meunier, Pinot noir and Chardonnay affected by Botrytis cinerea, Powdery mildew and Crustomyces subabruptus. METHODS: From analytical point of view, glycosylated precursors were extracted on C18 cartridges (Lichlorut RP-18, 500 mg),then cleaved by a beta-glycosidase enzyme (Rapidase Revelation Aroma, 40°C, Overnight) and aglycones were analyzed byGC-MS (FS and SIM mode) as reported by Schneider in 2001. RESULTS: For the first time, we identified a glycosylated fraction able to release fresh mushroom aroma (octen-3-one, octen-3-ol, octan-3-ol) in Meunier and Pinot noir musts. Indeed, contaminated musts of Pinot noir treated by beta glucosidase enzyme released three times more octen-3-one than control. In the samples infected with Powdery mildew, we didn’t observe any production of these glycosides. Botrytis cinerea seemed to decrease the level of octen-3-one glycoside(s) as soon as level contamination reached at least 1%. Finally, a Pinot noir must contaminated by Crustomyces subabruptus involved an increase of the glycosylated fraction responsible for the octen-3-one by 31% in comparison with uncontaminated sample. CONCLUSION: In conclusion, the identification of a glycosylated fraction able to release fresh mushroom aroma compounds opens avenue to better understand the source of this specific taint and constitutes the first step to help winemakers to avoid off-flavor.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Léa Delcros 

MHCS, Comité Champagne, Epernay, France,Teddy GODDET, SPO, Univ Montpellier INRAE, Institut agro, Montpellier, France  Sylvie COLLAS, Comité Champagne, Epernay, France  Marion HERVE, MHCS, Epernay, France  Bruno BLONDIN, SPO, Univ Montpellier INRAE, Institut agro, Montpellier, France  Aurélie ROLAND, SPO, Univ Montpellier, INRAE, Institut agro, Montpellier, France

Contact the author

Keywords

octen-3-one, octen-3-ol, glycosides, mushroom off-flavor, wine, botrytis cinerea, powdery mildew, crustomyces subabruptus

Citation

Related articles…

Volatile composition of Cabernet Sauvignon wines from Argentina, Portugal and Spain

Cabernet Sauvignon is one of the most cultivated grape varieties worldwide being grown in different environmental conditions due to its excellent adaptability. Volatile compounds deeply contribute to the sensory properties of wines therefore to wine quality. The aim of this work was to compare the aroma profile of Cabernet Sauvignon wines from different geographical areas and climatic conditions, namely from Argentina, Portugal and Spain, from the vintage 2022. In addition, the volatile composition of the Cabernet Sauvignon Portuguese wines from three vintages was evaluated.

The challenge of improving oenological quality in favorable conditions for productivity

Marselan (Cabernet-Sauvignon x Grenache), has been planted for more than 20 years now in Uruguay. Due to its good agronomic and oenological aptitudes under uruguayan conditions, it is currently the red variety with highest plantation rate. The objective of the study was to identify management practices, aimed at improving quality in highly productive vineyards, different fruit/leaf regulation methods were tested in southern Uruguay.

High pressure homogenization of fermentation lees: acceleration of yeast autolysis and evolution of white wine during sur-lies ageing

AIM: High pressure technologies represent a promising alternative to thermal treatments for improving quality and safety of liquid foods.

Monitoring of Pesticide Residues from Vine to Wine

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines.

THE INFLUENCE OF COMMERCIAL SACCHAROMYCES CEREVISIAE ON THE POLY-SACCHARIDES AND OTHER CHEMICAL PROFILES OF NEW ZEALAND PINOT NOIR WINES

Wine polysaccharides (PS) play an important role in balancing mouthfeel and stability of wine and even influence aroma volatility. Despite this, there is limited research into the effect of winemaking additives on the polysaccharide profile and other macromolecules of New Zealand (NZ) Pinot noir wine. In this study the influence of a selection of commercial S. cerevisiae strains on the chemical profile, including polysaccharides, of New Zealand Pinot noir (PN) wine was investigated. Research scale PN fermentations using five strains of commercially available S. cerevisiae (Lalvin EC1118 and RC212, Levuline BRG YSEO, Viallate Ferm R71 and R82) were undertaken. PS were qualified and quantified using HPLC-RID.

Macrowine 2021
IVES 9 IVES Conference Series 9 First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

First identification of a glycosylated fraction involved in mushroom-off-flavor in grapes: influence of B. cinerea, powdery mildew and C. subabruptus

Abstract

Content of the article

References

Section for all references

DOI:

Publication date: September 14, 2021

Issue: (ex: Issue: Terclim 2023)

Type: typeofpublication

Authors

author1, author2, author3

Presenting author

Description

List of affiliations ¹ ² ³

Contact the author

Email address (with mailto: link)

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

Citation

Related articles…

Revisión de estudios sobre suelos vitícolas de las tierras del Jerez

Dada la importancia de los suelos y de los substratos geológicos en la zonificación vitivinícola, los autores realizan una revisión de estudios sobre las formaciones más importantes en la D.O. Jerez-Xérès-Sherry y Manzanilla-Sanlúcar de Barrameda.

Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Cabernet Sauvignon is one of the most important winegrape varieties in Chile. However, temperature raise and decreased rainfall due to climate change can lead to grape quality decrease in certain areas. Amino acids are essential as nitrogen source for yeast but also directly affect grape quality serving as precursors of certain volatile compounds that enhance the wine bouquet. Besides, glutathione is an important tripeptide acting as antioxidant, preventing the appearance of browning pigments in must and exerts a protective effect in volatile compounds.

Interacción mesoclima-suelo en la calidad del vino de Cabernet-Sauvignon en las denominaciones de origen Priorato y Tarragona

Las condiciones heliotérmicas en España son en general favorables a alcanzar una elevada producción de azúcares en las bayas de prácticamente todas las variedades que se cultivan en nuestro país.

Deconstructing the soil component of terroir: from controversy to consensus

Wine terroir describes the collectively recognized relation between a geographical area and the distinctive organoleptic characteristics of the wines produced in it. The overriding objective in terroir studies is therefore to provide scientific proof relating the properties of terroir components to wine quality and typicity. In scientific circles, the role of climate (macro-, meso- and micro-) on grape and wine characteristics is well documented and accepted as the most critical. Moreover, there has been increasing interest in recent years about new elements with possible importance in shaping wine terroir like berry/leaf/soil microbiology or even aromatic plants in proximity to the vineyard conferring flavors to the grapes. However, the actual effect of these factors is also dependent on complex interactions with plant material (variety/clone, rootstock, vine age) and with human factors.
The contribution of soil, although a fundamental component of terroir and extremely popular among wine enthusiasts, remains a much-debated issue among researchers. The role of geology is probably the one mostly associated by consumers with the notion of terroir with different parent rocks considered to give birth to different wine styles. However, the relationship between wine properties and the underlying parent material raises a lot of controversy especially regarding the actual existence of rock-derived flavors in the wine (e.g. minerality). As far as the actual soil properties are concerned, the effect of soil physical properties is generally regarded as the most significant (e.g sandy soils being associated with lighter wines while those on clay with colored and tannic ones) mostly through control of water availability which ultimately modifies berry ripening conditions either directly by triggering biosynthetic pathways, or indirectly by altering vigor and yield components. The role of soil chemistry seems to be weakly associated to wine sensory characteristic, although N, K, S and Ca, but also soil pH, are often considered important in the overall soil effect.
Recently, in the light of evidence provided by precision agriculture studies reporting a high variability of vineyard soils, the spatial scale should also be taken into consideration in the evaluation of the soil effects on wines. While it is accepted that soil effects become more significant than climate on a local level, it is not clear whether these micro-variations of vineyard soils are determining in the terroir effect. Moreover, as terroir is not a set of only natural factors, the magnitude of the contribution of human-related factors (irrigation, fertilization, soil management) to the soil effect still remains ambiguous. Lastly, a major shortcoming of the majority of works about soil effects on wine characteristics is the absence of connection with actual vine physiological processes since all soil effects on grape and wine chemistry and sensorial properties are ultimately mediated through vine responses.
This article attempts to breakdown the main soil attributes involved in the terroir effect to suggest an improved understanding about soil’s true contribution to wine sensory characteristics. It is proposed that soil parameters per se are not as significant determining factors in the terroir effect but rather their mutual interactions as well as with other natural and human factors included in the terroir concept. Consequently, similarly to bioclimatic indices, composite soil indices (i.e. soil depth, water holding capacity, fertility, temperature etc), incorporating multiple soil parameters, might provide a more accurate and quantifiable means to assess the relative weight of the soil component in the terroir effect.

applicazione dei metodi isotopici e dell’analisi sensoriale negli studi sull’origine dei vini

Traceability of agro-alimentary products is very important to certify their origin. This work aimed to characterize wines obtained by the same cultivar (Nero d’Avola and Fiano) – grown in regions with different soil and climate conditions during three vintages (2003-2005) – employing isotopic analyses (NMR and IRMS) and sensory analyses.