Macrowine 2021
IVES 9 IVES Conference Series 9 Identification of riboflavin low producer yeasts to prevent the light-struck taste in white wines

Identification of riboflavin low producer yeasts to prevent the light-struck taste in white wines

Abstract

AIM: Wine quality maintenance during the storage is a fundamental aspect for both wine producers and consumers. Nowadays, great attention has been given to the light effect, causing detrimental changes of wine; indeed, light can induce off-flavours associated to the light-struck taste (LST)(1). This fault is due to photochemical oxidation processes in which riboflavin (RF) and methionine (Met) play an important role generating methanethiol (MeSH) and dimethyl disulphide (DMDS)(2), responsible of the unpleasant cabbage and onion-like odours that make wine undrinkable. Although it is well-known the contribution of yeasts in the final amount of these compounds in wine, microbiological strategies against the LTS defect limiting their release are not yet available. This study is part of the project “Innovative and sustainable approaches for the prevention of photo-induced defects in white wines and sparkling wines (Enofotoshield)” and in particular it aims at providing wineries with alternative microbiological approaches to counteract the LST.

METHODS: Four Saccharomyces cerevisiae strains of oenological interest (EC1118, IOC18, LS2 and AWRI796) have been compared to investigate the RF release during their growth in synthetic media (SMY and SMV) and Chardonnay must, simulating oenological conditions. Then, the RF release (including the derived flavones) was estimated by UPLC (Ultra-Performance Liquid Chromatography) analysis.

RESULTS: Results of this study revealed that RF production is influenced by the growth medium composition. Indeed, if the medium contains RF, the vitamin concentration increases over time while that of flavones remain constant; on the contrary, the opposite situation is verified in absence of RF. Moreover, investigations on other factors that could influence the RF release are still in progress (such as cell inoculum density, temperature, oxygen-limiting conditions, availability of nutrients).  Taking in consideration that a lower concentration than 80-100 μg/L could limit the LST development, the best identified condition in terms of RF release was the growth on the Chardonnay must (12,8 μg/L) compared to the two synthetic media SMY and SMV (102,4 μg/L and 316,5 μg/L, respectively).

CONCLUSIONS

This study paves the way for the development of new approaches that limit the impact on the wine aromatic profile. Indeed, the choice of the growth cultural medium is a relevant factor in terms of RF and Met production. The next steps of the study will be the analysis of the Met release and of the intracellular content of both RF and Met.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Alessandra Di Canito

University of Milan,Ileana Vigentini – University of Milan Daniela Fracassetti – University of Milan Antonio Tirelli – University of Milan  Roberto Foschino – University of Milan

Contact the author

Keywords

wine microbiology, light-struck taste, yeasts

Citation

Related articles…

Local adaptation tools to ensure the viticultural sustainability in a changing climate

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Sustainable fertilisation of the vineyard in Galicia (Spain)

Excessive fertilization of the vineyard leads to low quality grapes, increased costs and a negative impact on the environment. In order to establish an integrated management system aimed at a sustainable fertilization of the vineyards, nutritional reference levels were established. For this purpose, 30 representative vineyards of the Albariño variety were studied, in which soil and petiole analyses were carried out for two years and grape yield and quality at harvest were measured. In both years of study, soil pH, calcium, sodium and cation exchange capacity were positively correlated with calcium content and negatively correlated with manganese in grapes. Irrigated vineyards had higher levels of aluminium in soil and lower levels of calcium in petiole. Climatic conditions were very different in the years of the study. The year 2019 was colder than usual, in 2020 there was a marked water stress with high summer temperatures. This resulted in medium-high acidity in grapes in 2019 and low acidity in 2020, with sugar levels being similar both years. A very marked decrease in must amino nitrogen was observed in 2020, with ammonia nitrogen remaining stable. The correlation of acidity and sugar values in grapes with soil and petiole analysis data made it possible to establish reference levels for the nutritional diagnosis of the Albariño variety in this region. Based on these results, an easy-to-use TIC application is currently being created for grapegrowers, aimed at improving the sustainability of the vineyard through reasoned fertilization. This study has now been extended to other Galician vine varieties.

A blueprint for managing vine physiological balance at different spatial and temporal scales in Champagne

In Champagne, the vine adaptation to different climatic and technical changes during these last 20 years can be seen through physiological balance disruptions. These disruptions emphasize the general grapevine decline. Since the 2000s, among other nitrogen stress indicators, the must nitrogen has been decreasing. The combination of restricted mineral fertilizers and herbicide use, the growing variability of spring rainfall, the increasing thermal stress as well as the soil type heterogeneity are only a few underlying factors that trigger loss of physiological balance in the vineyards. It is important to weigh and quantify the impact of these factors on the vine. In order to do so, the Comité Champagne uses two key-tools: networking and modelization. The use of quantitative and harmonized ecophysiological indicators is necessary, especially in large spatial scales such as the Champagne appellation. A working group with different professional structures of Champagne has been launched by the Comité Champagne in order to create a common ecophysiology protocol and thus monitor the vine physiology, yearly, around 100 plots, with various cultural practices and types of soil. The use of crop modelling to follow the vine physiological balance within different pedoclimatic conditions enables to understand the present balance but also predict the possible disruptions to come in future climatic scenarios. The physiological references created each year through the working group, benefit the calibration of the STICS model used in Champagne. In return, the model delivers ecophysiology indicators, on a daily scale and can be used on very different types of soils. This study will present the bottom-up method used to give accurate information on the impacts of soil, climate and cultural practices on vine physiology.

Climate ethnography and wine environmental futures

Globalisation and climate change have radically transformed world wine production upsetting the established order of wine ecologies. Ecological risks and the future of traditional agricultural systems are widely debated in anthropology, but very little is understood of the particular challenges posed by climate change to viticulture which is seen by many as the canary in the coalmine of global agriculture. Moreover, wine as a globalised embedded commodity provides a particularly telling example for the study of climate change having already attracted early scientific attention. Studies of climate change in viticulture have focused primarily on the production of systematic models of adaptation and vulnerability, while the human and cultural factors, which are key to adaptation and sustainable futures, are largely missing. Climate experts have been unanimous in recognising the urgent need for a better understanding of the complex dynamics that shape how climate change is experienced and responded to by human systems. Yet this call has not yet been addressed. Climate ethnography, coined by the anthropologist Susan Crate (2011), aims to bridge this growing disjuncture between climate science and everyday life through the exploration of the social meaning of climate change. It seeks to investigate the confrontation of its social salience in different locations and under different environmental guises (Goodman 2018: 340). By understanding how wine producers make sense of the world (and the environment) and act in it, it proposes to focus on the co-production of interdisciplinary knowledge by identifying and foreshadowing problems (Goodman 2018: 342; Goodman & Marshall 2018). It seeks to offer an original, transformative and contrasted perspective to climate change scenarios by investigating human agency -individual or collective- in all its social, political and cultural diversity. An anthropological approach founded on detailed ethnographies of wine production is ideally placed to address economic, social and cultural disruptions caused by the emergence of these new environmental challenges. Indeed, the community of experts in environmental change have recently called for research that will encompass the human dimension and for more broad-based, integrated through interdisciplinarity, useful knowledge (Castree & al 2014). My paper seeks to engage with climate ethnography and discuss what it brings to the study of wine environmental futures while exploring the limitations of the anthropological environmental approach.