Macrowine 2021
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2021 9 Chemical diversity of 'special' wine styles: fortified wines, passito style, botrytized and ice wines, orange wines, sparkling wines 9 Polyphenol targeted and untargeted metabolomics on rosé wines : impact of protein fining on polyphenolic composition and color

Polyphenol targeted and untargeted metabolomics on rosé wines : impact of protein fining on polyphenolic composition and color

Abstract

Color is one of the key elements in the marketing of rosé wines[1]. Their broad range of color is due to the presence of red pigments (i.e. anthocyanins and their derivatives) and yellow pigments, likely including polyphenol oxidation products. Clarifying agents are widely used in the winemaking industry to enhance wine stability and to modulate wine color by binding and precipitating polyphenols[2]. During this study, the impact of four different fining agents (i.e. two vegetal proteins, potatoe and pea proteins, an animal protein, casein, and a synthetic polymer, polyvinylpolypyrrolidone, PVPP) on Syrah Rose wine color and phenolic composition (especially pigments) was investigated. Color was characterized by spectrophotometry analysis using the CIELab system in addition to absorbance data. Fining using PVPP had the highest impact on redness (a*) and lightness (L*) parameters, whereas patatin strongly reduced the yellow component (b*) of the wine color. In parallel, the concentration of 125 phenolic compounds including 85 anthocyanins and derived pigments was determined by Ultra High Performance Liquid Chromatography coupled to elestrospray ionisaion triple-quadrupole Mass Spectrometry (UHPLC-QqQ-ESI-MS) in the Multiple Reaction Monitoring mode[3] . Results confirmed the affinity of PVPP towards flavan-3-ols and anthocyanins, especially coumaroylated anthocyanins demonstrated earlier[4]. Chemometrics analysis of the color and composition data revealed a link between redness (a*) and lightness (L*) related to native anthocyanin and flavan-3-ol concentrations. However, no specific marker was associated to patatin fining, suggesting the involvement of other pigments in the yellow component (b*). Additional data was acquired on the same set of samples by untargeted metabolomics using Ultra High Performance Liquid Chromatography coupled to an High Resolution Mass Spectrometer (UHPLC-HR-MS). Our results corroborate those of targeted analysis, demonstrating particular affinity of PVPP for native anthocyanins and flavan-3-ol but also flavonols and stilbenes. Markers of each fining treatment were also identified. PVPP fining treatment revealed a sharp decrease in the rose wine color, especially on the redness (a*) component linked to losses of phenolic compounds such as native anthocyanin. Further investigations aiming at revealing markers of the yellow component (b*) from untargeted analysis data are under way.

DOI:

Publication date: September 16, 2021

Issue: Macrowine 2021

Type: Article

Authors

Cécile Leborgne

SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier  Institut Français de la Vigne et du Vin, Centre du Rosé, Vidauban,Ashley Carty, SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier  Aurélie Chevalier, Institut Français de la Vigne et du Vin, Centre du Rosé, Vidauban  Arnaud Verbaere, SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier  Matthias Bougreau, Institut Français de la Vigne et du Vin, Centre du Rosé, Vidauban  Jean-Claude Boulet, SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier  Nicolas Sommerer, SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier   Gilles Masson, Institut Français de la Vigne et du Vin, Centre du Rosé, Vidauban  Jean-Roch Mouret, SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier  Véronique Cheynier, SPO, Univ Montpellier, INRAE, Montpellier SupAgro, Montpellier

Contact the author

Keywords

rosé wine – color – polyphenols – metabolomics – targeted & untargeted analysis

Citation

Related articles…

OENOLOGICAL TANNINS FOR PREVENTING THE LIGHT-STRUCK TASTE IN WHITE AND ROSÉ WINES

The light exposure of wine can be detrimental as a relevant loss of aromas takes place [1] and light-induced reactions can occur. The latter involves riboflavin (RF), a photosensitive compound, that is fully reduced by acquiring two electrons. When the electron-donor is methionine, the light-struck taste (LST) can appear leading to cooked cabbage, onion and garlic odours-like [2]. The use of oenological tannins can limit the appearance of LST in both model wine [3] and white wine [4]. This research aimed to evaluate the impact of certain oenological tannins, selected in a previous study as the most effective against LST [5], in both white and rosé wines.

Development of analytical sampling technique to study the aroma profile of Pinot Noir wine

A novel and efficient Dispersive Liquid-Liquid Microextraction (DLLME) method coupled with gas chromatography–mass spectrometry (GC–MS) was developed to determine 33 key aroma compounds (esters, alcohols, aldehydes, terpenes, norisoprenoids, fatty acids and phenols) present in Pinot noir (PN) wine. Four critical parameters including extraction solvent type, disperse solvent type, extraction solvent volume and disperse solvent volume were optimised with the aid of D-optimal design.

Application of plant growth regulators on Vitis vinifera L var. Mouchtaro affect berry quality characteristics & associated microbial communities

The phenolic profile of the red grapevine varieties berries is a key quality factor and several techniques have been applied to improve it (Perez-Lamela et al., 2007; Singh SK and Sharma, 2010). The last decade the application of resistance elicitors and phytohormones is an innovative viticultural technique (Paladines-Quezada et al., 2021; Alenazi et al., 2019).In the present study, leaves and berries of a Greek red indigenous variety (Mouhtaro) sprayed with two elicitors, benzothiadiazole and chitosan and a plant hormone abscisic acid, during veraison.

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].

Using unmanned aerial vehicle with multispectral camara to efficiently and precisely monitor the incidence of downy mildew and technical maturity of beibinghong (vitis amurensis Rpru.) grapes 

Multispectral unmanned aerial vehicle (UAV) has been successfully applied in monitoring vine vegetative growth, however, there are still potential relationships between remote sensing vegetation indexes (vis) and vine condition or grape quality, so it is worthy a deeper investigation to make a better use of UAV. One of the purposes of the study is to find out vis that could denote the severity of downy mildew (DM), so that precise and differentiated control strategies would be adopted subsequently.