Influence of the irrigation period in Tempranillo grapevine, under the edaphoclimatic conditions of the Duero river valley

Foliar application of urea has proven to be an effective method for increasing the amino acid content in grapes, especially when the vineyard has additional nitrogen needs. These treatments can prevent problems of stucking fermentation during winemaking.

Study the influence of sample preparation methods on the volatile organic compounds (VOCs) profiling for fermentative beverages by GCxGC-TOFMS analysis. METHODS: Five common sample preparation methods were tested on pooled red wine, white wine, cider, and beer. Studied methods were DHS, Liquid-liquid extraction, mSBSE, SPE and SPME. VOCs were analyzed by GCxGC-TOFMS followed by data analysis with ChromaTOF. RESULTS: The volatile organic compounds (VOCs) profiling results were very dependent on the sample preparation methods.

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’. In symptomatic grapevines cv. ‘Zweigelt’ infected with ‘Ca. P. solani’ compared with uninfected grapevines, metabolic pathways associated with phosphorylated sugar production were induced both at the transcriptional level and at the level of activity of the corresponding enzymes (Dermastia et al., 2021, Int. J. Mol. Sci. 22: 3531). In particular, the expression of gene coding for phosphoglucoisomerase was upregulated, resulting in increased phosphoglucoisomerase enzyme activity.

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.

Aim: To investigate the possibility of utilising simultaneous measurements of absorbance-transmittance and fluorescence excitation-emission matrix (A-TEEM) combined with chemometrics, as a robust method that gives rapid results for classification of wines from different regions of South Australia according to their Geographical Indication (GI), and to gain insight into the effect of terroir on inter regional variation.