Classification of the wine-growing environment of Central Mancha (Spain). First works

Extraction of anthocyanins and tannins have been studied for two grape varieties, Carignan and Grenache, two maturation levels and two vintages, in model solutions and in wines, using UHPLC-MS/MS in the MRM mode  and HPSEC.

Valbelluna valley is an area located in the northeastern Italy. It is extended from the East-West between Feltre and Belluno, along the Piave waterway and enclosed between Cansiglio valley on the South and the Dolomites in the North. Here, the villages of Limana and Trichiana are present, which are considered for decades potentially interesting areas to aim a niche production with own particular properties.The position of this area, its sun exposition, its soil composition and the microclimate, are ideal factors to obtain vines and consequently wines with unique features especially regarding the diversity and complexity aroma.

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

The research is part of the “Ecovinegoals” project, financed by Interreg Adrion funds. It aims to encourage the adoption and dissemination of agroecological practices in intensive wine-growing areas. The study focuses on cost analysis of the wine-growing landscape enhancement in an organic winery in order to provide a useful tool for winemakers to direct their investments in green infrastructures. One of the Italian pilot areas of the Ecovinegoals project is the Venezia Biodistrict, characterized by viticulture in a flat reclamation area of 105,800 hectares.