AIM: Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. Its simple handling and no need for dedicated equipment together with an evaluation of the amplification event by in-tube detection make this method advantageous and economically affordable for on-site investigations in the industry.

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Tannins are a heterogenous class of polymeric phenolics found in grapes, oak barrels and wine. In red wine tannins are primarily responsible for astringency, though they also have an important role in reacting with and stabilizing pigments. There are numerous sub-classes of tannins found in wine but they all share structural heterogeneity within each sub-class, with varied polymer composition, configuration and length.

Numerous methodologies exist for the quantification of tannins, however, protein precipitation using bovine serum albumin has proved itself useful due to its strong correlation to the sensory perception of astringency and the basic instruments required for the method. Though the method can yield valuable insights into tannin composition, it cannot be automated easily and necessitates well-trained personnel.

Climate change is having a significant impact on the wine industry through more regular drought conditions, fires, and heat events, leading to crop loss. Furthermore, these events can reduce overall quality of the fruit, even when crop yields are not impacted. Anthocyanins are considered one of the most important classes of compounds for red wine production and are known to be sensitive to vine water status and heat events.

The ongoing climate change results in increasing mean air temperature, which is manifested by weather extremes or sudden changes between drought and local heavy rainfalls. These changing conditions are especially challenging for the established grapevine varieties growing under cool climate conditions due to an increased biotic infection pressure. Thus, the scope of most grapevine breeding programs is the selection of mildew fungus-resistant and climatic adapted grapevines with balanced, healthy yield and outstanding wine quality. Since no resistances or candidate genes have yet been described for Botrytis bunch rot (BBR), physical-mechanical traits like berry size and thick, impermeable berry cuticles phenotyped with high-throughput sensors represent novel effective parameters to predict BBR.