IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

Abstract

Extraction of anthocyanins and tannins have been studied for two grape varieties, Carignan and Grenache, two maturation levels and two vintages, in model solutions and in wines, using UHPLC-MS/MS in the MRM mode  and HPSEC. The cell wall polysaccharides were characterized using the neutral sugar composition after depolymerization and the comprehensive microarray polymer profiling (CoMPP).
Carignan was richer than Grenache in anthocyanins for both years. Berry anthocyanins were mainly non acylated and para coumaroylated.  In Carignan, p.coumaroylated were found in higher quantities than non acylated. Maturation led to an increase of quantities of non acylated anthocyanins for Carignan and Grenache, and a slight decrease of p.coumaroylated for Carignan. No significant difference of their tannin composition was observed.
The extraction yields of non acylated anthocyanins in model solutions and in wines were higher than those of tannins. Percents of recoveries of p.coumaroylated anthocyanins were lower than non acylated anthocyanins and tannins, and lower in model solutions than in wine. Recoveries were higher in 2019 than in 2018.
Correlations were observed between non acylated, p.coumaroylated and tannins concentrations in model solutions and wines, not in berries. P.coumaroylated anthocyanins recoveries were lower in model solutions than in wines. The cell wall structure was related to the mechanism of extraction. Extraction of anthocyanins and tannins was correlated to high levels of homogalacturonans partially esterified in the skins (e.g. LM19-CDTA-skin) but low levels in the pulps, and by low levels of extensins in the skin(e.g. JIM11-NaOH-pulp) but high levels in the pulps. Arabinose % was correlated positively, mannose % and glucose % negatively to the recovery of all anthocyanins and tannins in model solution, to p.coumaroylated anthocyanins only in wines. These results trigger questions.
Firstly, the lower recovery of p.coumaroylated anthocyanins may be due to the hydrophobicity of the coumaroyl unit, modifying their interactions with other polyphenols and/or with the cell walls.
Secondly, p.coumaroylated anthocyanin recoveries were very different in model solutions and in wines. Pulp, seeds and/or yeasts present in wines should play a role in their extractibility.
Thirdly, anthocyanins/tannins extracted in model solutions/wines were correlated to several parameters describing the cell walls, among them their compositions measured by the neutral sugars and their structures measured by the CoMPPs. To conclude, this study confirms with more details the major role that play cell walls in the extraction of anthocyanins and tannins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Boulet Jean-Claude1, Abi-Habib, Carrillo Stéphanie, Roi Stéphanie, Verbaere Arnaud, Meudec Emmanuelle, Rattier Anaïs, Ducasse Marie-Agnès, Jorgensen Bodil, Hansen Jeanett, Le Gall Sophie, Poncet-Legrand Céline, Cheynier Véronique, Doce Thierry and Verneht Aude

1SPO, INRAE, Univ.Montpellier, Institut Agro Montpellier Supagro, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France
2INRAE, PROBE infrastructure, PFP facility, 34070 Montpellier, Campus Supagro, Bâtiment 28, 2 Place Viala, 34060 Montpellier cedex 2, France

Contact the author

Keywords

extraction, polyphenols, polysaccharides, comprehensive microarrray polymer profiling, wine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Analysis of the daily minimum temperatures variability in the Casablanca Valley, Chile

The Casablanca Valley (CV) has a complex topography and is located near the Pacific Ocean. These factors generate important climatic differences in relation to other wine producing zones of Central Chile.

High density balsamic vinegar: application of stable isotope ratio analysis to determine watering down.

Aceto balsamico di Modena IGP (ABM) is an Italian worldwide appreciated PGI (Protected Geographical Indication) vinegar,  obtained from cooked and/or concentrated grape must (at least 20% of the volume), with the addition of at least 10% of wine vinegar and a maximum 2% of caramel for color stability (EU Reg. 583/ 2009).

Flooding responses on grapevine: a physiological, transcriptional and metabolic perspective

Studies on model plants have shown that temporary soil flooding exposes roots to a significant hypoxic stress resulting in metabolic re-programming, accumulation of toxic metabolites and hormonal imbalance. To date, physiological and transcriptional responses to flooding in grapevine are poorly characterized. To fill this gap, we aimed to gain insights into the transcriptional and metabolic changes induced by flooding on grapevine roots (K5BB rootstocks), on which cv Sauvignon blanc (Vitis vinifera L.) plants were grafted.

Ripening characterization and modelling of Listan negro grape in Spain using a regression analysis

The professional winegrower usually selects the harvest date considering several elements, such as the vine stem and berry colour, the flavour, appearance and grain elasticity. Nowadays these elements have turned old fashioned.

A mechanistic investigation of H/D scrambling processes in flavonoids

Several classes of flavonoids, such as anthocyanins, flavonols, flavanols and flavones, undergo a slow H/D exchange on aromatic ring A, leading to full deuteration at positions C(6) and C(8). Within the flavanol class, H-C(6) and H-C(8) of catechin and epicatechin are slowly exchanged in D2O to the corresponding deuterated analogues; even quercetin, a relevant flavonol representative, shows the same behaviour in a D2O/DMSOd6 1:1 solution. Detailed kinetic measurements of these H/D scrambling processes are here reported by exploiting the time-dependent changes of their peak areas in the 1H-NMR spectra taken at different temperatures. A unifying reaction mechanism is also proposed based on our detailed kinetic observations, even taking into account pH and solvent effects. Molecular modelling and QM calculations were also carried out to shed more light on several molecular details of the proposed mechanism.

IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

The impact of cell wall composition of the extraction of anthocyanins and tannins from grape berries

Abstract

Content of the article

References

Section for all references

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: typeofthepublication

Authors

author1, author2, author3

Presenting author

Description

List of affiliations ¹ ² ³

Contact the author

Email address (with mailto: link)

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Contribution of grape seeds to evolution of acetaldehyde, pigments and tannins reactive towards salivary proteins of red wine over time

This study investigated the impact of the gsk/gse ratio on the evolution of acetaldehyde and of major phenolic compounds of aglianico wine in wine like solution and real wine. Four model solutions and the correspondant control wines were prepared. The natural weight ratio between grape skins and seeds was determined on the real grapes, and a control wine was obtained from those.

Effect of interspecific yeast hybrids for secondary in-bottle alcoholic fermentation of english sparkling wines

In sparkling winemaking several yeasts can be used to perform the primary alcoholic fermentation that leads to the elaboration of the base wine. However, only a few Saccharomyces cerevisiae yeast strains are regularly used for the secondary in-bottle alcoholic fermentation 1. Recently, advances in yeast development programs have resulted in new breeds of interspecific wine yeast hybrids that ferment efficiently while producing novel flavours and aromas 2. In this work, sparkling wines produced using interspecific yeast hybrids for the secondary in-bottle alcoholic fermentation have been chemically and sensorially characterized.METHODS: Three commercial English base wines have been prepared for secondary in-bottle alcoholic fermentation with different yeast strains, including two commercial and several novel interspecific hybrids derived from Saccharomyces species not traditionally used in sparkling winemaking. After 12 months of lees ageing, the 14 wines produced were analysed for their chemical and macromolecular composition 3,4, phenolic profile 5, foaming and viscosity properties [6]. The analytical data were supplemented with a sensory analysis.

Differential gene expression and novel gene models in 110 Richter uncovered through RNA Sequencing of roots under stress

The appearance of the Phylloxera pest in the 19th century in Europe caused dramatical damages in grapevine diversity. To mitigate these losses, grapevine growers resorted to using crosses of different Vitis species, such as 110 Richter (110R) (V. berlandieri x V. rupestris), which has been invaluable for studying adaptations to stress responses in vineyards. Recently, a high quality chromosome scale assembly of 110R was released, but the available gene models were predicted without using as evidence transcriptional sequences obtained from roots, that are crucial organs in rootstock, and they may express certain genes exclusively. Therefore, we employed RNA sequencing reads of 110R roots under different stress conditions to predict new gene models in each haplotype of 110R under different stresses.

Analyzing firms’ dynamic capabilities to identify the actions for a sustainable future of the Italian wine sector

The UN Agenda 2030 for Sustainable Development, a global plan for a better future, requires actions.

Biodiversity and biocontrol ability of Trichoderma natural populations in soil vineyards from Castilla y León region (Spain)

Trichoderma is a microorganism present in many agricultural soils and some of its species could be used as natural biological control agents. In this work, the presence of natural populations of Trichoderma was estimated in soil vineyard and its biocontrol capacity against Phaeoacremonium minimum, one of the main agent causals of grapevine trunk diseases instead of using pesticides. Moreover, physicochemical variables in soil such as pH, organic matter and nutrients were evaluated to determine a possible correlation to natural populations of Trichoderma.