IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Structural composition of polymeric polyphenols of red wine after long-term ageing: effect of vinification technology

Structural composition of polymeric polyphenols of red wine after long-term ageing: effect of vinification technology

Abstract

Aged red wines possess phenolic composition very different from young ones due to the transformations among native grape phenolics and the formation of new polymeric polyphenols during aging process. In this work, Syrah red wines were made by different winemaking technologies, i.e., traditional fermentation on skin (total 7 days of maceration), prolonged maceration with addition of extra skins at the end of traditional fermentation (total 14 days of maceration) and prolonged maceration with addition of extra stems at the end of traditional fermentation (total 14 days of maceration). After 8 years of ageing in bottle, the structural composition of polymeric polyphenols in these wines was comprehensively analysed through different degradation methods (hydrochloric acid hydrolysis, NaOH hydrolysis and Benzyl mercaptan hydrolysis), followed by HPLC-FT-ICR-MS, HPLC/UPLC-MS analysis. The results showed that the molecules of polymeric polyphenols in the aged red wines were composed of not only proanthocyanidins but also anthocyanins, amino acids and phenolic acids. The percentages of the constitutive units of the polymeric polyphenol molecules in these wines varied considerably, being catechin (7.1 – 14.9%), epicatechin (74.5 – 78.2%), epicatechin-3-O-gallate (5.8 – 12.2%), amino acids (0.7 – 1.5%), phenolic acids (0.0 – 0.9%) anthocyanins (0.1 – 0.4%) and epigallocatechin (0.7 – 4.7%),  depending on the type of the winemaking technologies. Catechin, epicatechin and epicatechin-3-O-gallate were presented as both terminal and extension units, with the latter predominant, while amino acids, phenolic acids and anthocyanins were found to be presented exclusively as terminal units and epigallocatechin was found to be presented exclusively as extension units. Comparing with the wine made by traditional fermentation on skin, the lower phenolic acids and anthocyanins units was found in the wine made by prolonged fermentation/maceration with skin and with stem. The prolonged fermentation/maceration with skin was found to have highest amino acids units. On the other hand, different vinification technologies affected the mean polymerization degrees (mDP) of polymeric polyphenols in the aged red wines, being mDP 25.2 for the control one, mDP 13.1 for the wine made by the prolonged fermentation with skin and mDP 15.7 for the prolonged fermention with stem. These results indicated that, different winemaking technologies affect significantly the structural features of polymeric polyphenols.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Sun Baoshan1, Jian Zhao3, Tingting Yang1, Martins Patrícia2, Ramos João4 and Lingxi Li1

1School of Functional Food and Wine, Shenyang Pharmaceutical University
2Pólo Dois Portos, Instituto Nacional de Investigação Agrária e Veterinária, I.P.

3School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University
4Departamento de Enologia, Herdade do Esporão, Reguengos de Monsaraz

Contact the author

Keywords

polymeric polyphenols; winemaking technology; structural composition; aged red wine

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Recognition of terroir in american viticultural areas

Un’ Area di Viticultura Americana, detta AVA, è una regione vinicola delimitata ed è dis­tinguibile da caratteristiche geografiche i cui confini sono stati definiti da regolamenti. Il sistema AVA rappresenta un ‘accettazione del concetto di terroir (terreno), come dimostra­no gli studi che confermano il carattere regionale dei vini AVA e dalla sviluppo di sub­denominazioni più relazionate al terreno.

Fining-Derived Allergens in Wine: from Detection to Quantification

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition).

Phenological stage dependency of Cabernet Sauvignon and Grenache response to water and nutrient limitation 

As the frequency and intensity of drought events increase, understanding the mechanisms of plant resilience to water deficit is crucial. To maintain an appropriate plant yield, a common practice is the application of high amounts of fertilizers with negative environmental impacts. The single and combined effect of water deficit and nutrient availability, namely nitrogen (N) and potassium (K), in Vitis Vinifera L. cv. Cabernet Sauvignon and Grenache was evaluated. Two-year-old grapevine plants grafted on SO4 rootstock were transferred in pots under semi-environmental conditions. During the growing season, plants were either maintained well-watered (100% ETc) or subjected to a controlled water deficit irrigation (33% ETc).

Using atmospheric and statistical models to understand local climate and assess spatial temperature variability at a fine scale over the Stellenbosch wine district, South Africa

Atmospheric and statistical models were used to increase understanding of potential climatic impacts, resulting from mesoscale physical processes that cause significant temperature variability for viticulture within the Stellenbosch Wine of Origin district. Hourly temperature values from 16 automatic weather stations and 40 tinytag data loggers located in the vineyards were analysed.

Activation of retrotransposition in grapevine

Retrotransposons, particularly of the Ty-Copia and Ty-Gypsy superfamilies, represent the most abundant and widespread transposons in many plant genomes. Grapevine is no exception and it is clear that these mobile elements have played a major role in the evolution of Vitaceae genomes. While speculation abounds around the possible role of transposons in plant genomes, outside of the rather obvious involvement of retrotransposition in fueling genome expansion, there is little clarity of the actual role these elements have in both developing new genetic variation and in modulating epigenetic responses within genomes to changing climate. To this end we have been exploring de-novo assembled Sauvignon blanc and Pinot noir genomes with a view to catalogue retrotransposon loci to determine the structural intactness and thus age of insertion variation across a small number of clonal linages of these 2 varietals in an attempt to identify ‘live’ TE loci.