The limonene-derived mint aroma compounds in red wines. Recent advances on analytical, chemical aspects and sensory aspects

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure.

With its Farm-to-Fork Strategy, which is a part of the European Green Deal, the European Union aims at reducing the amount of pesticides used in agriculture by 50% until 2030. As viticulture uses around 70% of the fungicides in the EU, there is substantial pressure on winemakers to reduce their pesticide input. On top of the political goal, winegrowers face increased pressure from the public demanding a more sustainable production of wine.

The studies developed by INRA and UV, in Angers, concern wine-growing areas and their optimized management, both from an agro-viticultural and oenological point of view. Previous work (Morlat, 1989) made it possible to give a scientific dimension to the concept of viticultural terroir and demonstrated the considerable influence of this production factor on the quality and typicity of wines (Asselin et al, 1992 ) . A methodology for the integrated characterization of terroirs, based on the “Basic Terroir Natural Unit” (considered as the smallest spatial unit of territory usable in practice, and in which the response of the vine is homogeneous), has been development (Riou et al , 1995).

Measurement of carbon isotope discrimination in berry juice sugars at maturity (δ13C) provides an integrated assessment of water use efficiency (WUE) during the period of berry ripening, and when collected over multiple seasons can be used as an indication of drought stress response. Berry juice δ13C measurements were carried out on 48 different varieties planted in a common garden experiment in Bordeaux, France from 2014 through 2021 and were paired with midday and predawn leaf water potential measurements on the same vines in a subset of six varieties. The aim was to discriminate a large panel of varieties based on their stomatal behaviour and potentially identify hydraulic traits characterizing drought tolerance by comparing δ13C and hydroscapes (the visualisation of plant stomatal behaviour as a response to predawn water potential). Cluster analysis found that δ13C values are likely affected by the differing phenology of each variety, resulting in berry ripening of different varieties taking place under different stress conditions within the same year. We accounted for these phenological differences and found that cluster analysis based on specific δ13C metrics created a classification of varieties that corresponds well to our current empirical understanding of their relative drought tolerances. In addition, we analysed the water potential regulation of the subset of six varieties (using the hydroscape approach) and found that it was well correlated with some δ13C metrics. Surprisingly, a variety’s water potential regulation (specifically its minimum critical leaf water potential under water deficit) was strongly correlated to δ13C values under well-watered conditions, suggesting that base WUE may have a stronger impact on drought tolerance than WUE under water deficit. These results give strong insights on the innate WUE of a very large panel of varieties and suggest that studies of drought tolerance should include traits expressed under non-limiting conditions.

A study of the physiological and agronomical behaviour of the vine (cv. Chasselas) was conducted between 2001 and 2003 by the Swiss Federal Research Station for Plant Production at Changins (Agroscope RAC Changins) on various wine-growing farms (terroirs) in the Canton of Vaud (Switzerland), as part of a study project on Vaudois