Macrowine 2021
IVES 9 IVES Conference Series 9 Defining the mechanisms and impact of winemaking treatments on tannin and polysaccharides in red wine: recent progress in creating diverse styles

Defining the mechanisms and impact of winemaking treatments on tannin and polysaccharides in red wine: recent progress in creating diverse styles

Abstract

Tannin and polysaccharide concentration and composition is important in defining the texture of red wines, but can vary due to factors such as cultivar, region, grape ripeness, viticultural practices and winemaking techniques. However, the concentration and composition of these macromolecules is dependent not only on grape tannin and polysaccharide concentration and composition, but also their extractability and, in the case of polysaccharides, their formation by yeast. Through studies into the influence of grape maturity, winemaking and sensory impacts of red grape polysaccharides, seed and skin tannins, recent research in our laboratory has shown that the processes involved in the extraction of these macromolecules from grapes and their retention in wine are very complex. In particular, the isolation and characterisation of polysaccharides and cell wall material (fibre) has shown that grape cell wall material (CWM) can bind tannins and modify the amount and type of tannins retained in wine. The action of enzymes, yeast and certain winemaking treatments on cell wall material can also profoundly influence the amount and types of polysaccharides retained in wine.These recent advances present new factors for consideration in grape selection and processing during winemaking which allow winemakers to more rigorously control colour and mouth-feel in red wines. This presentation with summarise recent studies in our laboratory that have focused on evaluating yeast strains and maceration processes during winemaking as tools to alter wine macromolecule concentration and composition. In wine made in the 2014 vintage it was found that the choice of yeast strain (10 yeast strains were benchmarked) resulted in highly variable polysaccharide and tannin concentrations. At the end of primary fermentation, the two yeasts which yielded highest wine tannin concentrations (1.5 g/L) resulted in wine with the lowest (0.45 g/L) and highest (0.66 g/L) polysaccharide concentrations respectively. It was found that the wine with the highest polysaccharide and tannin was associated with a transient release of pectic polysaccharides rich in galacturonic acid and arabinose from the grapes, suggesting pectolytic activity in yeast. Based on leads from this trial, in 2015 an experiment was performed to investigate the interactive effect of maceration time (7 vs 30 days), macerating enzyme and yeast strains (‘high-tannin’ vs ‘low-tannin’ yeast) on wine macromolecules in 50 kg Shiraz ferments. At 30 days, post maceration, no effect of yeast strain on polysaccharide concentration or composition was observed, although strong effects were found for enzyme application and maceration time. Results also show the combined use of yeast strain and maceration techniques can have a marked effect on wine tannin, but 30dM achieved the most significant shift in tannin concentration and molecular mass. The mechanisms by which these effects may occur will also be presented.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Paul Smith*, Chris Curtin, Keren Bindon, Mark Solomon, Stella Kassara

*The Australian Wine Research Institute

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of non-Saccharomyces yeast and lactic acid bacteria on selected sensory attributes and polyphenols of Syrah wines

Consumers predominantly use visual, aromatic and texture cues as quality/preference indicators to describe olfactory sensations. In this study, the effect of micro-organism in wine production was investigated using analytical and sensory techniques to achieve relevant analytical characterisation. Selected anthocyanins, flavan-3-ols, flavonols and phenolic acids were quantified in Syrah wines using RP-HPLC-DAD. Standard oenological parameters were also measured. Syrah grape must was fermented with various combinations of Saccharomyces cerevisiae (S. cerevisiae) and non-Saccharomyces (Metschnikowia pulcherrima or Hanseniaspora uvarum) yeasts, which was followed by sequential inoculation of lactic acid bacteria (LAB) (Oenococcus oeni or Lactobacillus plantarum).

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T

Use of glutathione under different grape processing and winemaking conditions and its impact on the formation of sulfide off-flavors, colour, and sensory characteristics of Riesling, Sauvignon blanc, and Chardonnay

The use of glutathione (GSH) in winemaking has been legitimated recently, according to OIV resolutions OENO 445-2015 and OENO 446-2015 a maximum dose of 20 mg/L is now allowed to use in must and wine. Several studies have proven the benefits of GSH, predominantly in Sauvignon blanc. Thus, oxidative coloration of must and wine is limited, aroma compounds such as volatile thiols are preserved, and the development of ageing flavors such as sotolon and 2-aminoacetophenone is impeded. The protective effect may be explained by the high affinity of GSH to bind o-quinones which are formed during phenolic oxidation and which are known to initiate browning and other oxidative changes. Some researchers have proposed the hydroxycinnamic acid to GSH ratio (HGR) as an indicator of oxidation susceptibility of must and could show that lower ratios yielded lighter musts.

Study of the colour and phenolic evolution of three different tannin/anthocyanin ratios over time in a model wine

Phenolic compounds are important quality indicators in red wine. A large number of polyphenols play an important role in wine development, contributing to the colour and the sensory perception of the wines. Anthocyanins are the pigments responsible for the colour in young red wines while tannins are the principal contributors to the bitterness and the astringency of the wines. Wine polyphenols are considered more complex molecules than grape phenolics, due to the enormous number of chemical reactions which take place during the entire winemaking process and storage, forming more stable compounds.

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels.