terclim by ICS banner
IVES 9 IVES Conference Series 9 Juvenile-to-adult vegetative phase transition in grapevine 

Juvenile-to-adult vegetative phase transition in grapevine 

Abstract

The sequential activity of miR156 and miR172 controls the juvenile to adult phase transition in many plant species, where miR156 abundance decreases while miR172 increases along plant development. Very little is known about phase transition in horticultural woody species, which show substantially long vegetative phases. In grapevine, phase transition seems to be dissociated, displaying a first transition from juvenile to adult vegetative state in the first year, coincident with tendril differentiation and a subsequent induction of inflorescences in place of some of tendrils in later years under flowering inductive environmental conditions. Since grapevine is a highly heterozygous species, the generation of genetically homogeneous material for replicated transcriptomic analyses from seed-derived plants was a main challenge. Here, we present a detailed global gene expression analysis of the juvenile-to-adult phase transition during the development of grapevine plantlets grown from seeds. The RNA-seq analysis demonstrated that miR156 was significantly repressed in the grapevine’s adult phase, where the appearance of tendrils acts as a marker of the transition. Consistent with the results reported in other species, we observed the activation of several SPL genes, known to be targets of miR156, and providing evidence for the conservation of the regulatory module miR156-SPLs in grapevine. However, no variation was detected in the expression of miR172, a key determinant in the transition to flowering in other species. This could be explained considering that grapevines do not flower during the first years of growth. Interestingly, we were still able to observe the overexpression of several genes known to be involved in the floral meristem identity transition which were also been detected along tendril development, consistently with the proposed common ontogenetic origin of tendrils and inflorescences in the Vitaceae family.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Diego Lijavetzky1*, Yolanda Ferradás2,3, Carolina Royo3, José Miguel Martínez-Zapater3

1Instituto de Biología Agrícola de Mendoza (IBAM, CONICET-UNCuyo), Almirante Brown 500, M5528AHB. Chacras de Coria, Mendoza, Argentina
2Departamento de Biología Funcional, Universidade de Santiago de Compostela, Santiago de Compostela, Spain
3Instituto de Ciencias de la Vid y del Vino, Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, La Rioja, Spain

Contact the author*

Keywords

phase change, juvenile phase, flowering transition, tendril development, miRNA, RNA-seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of SO2 addition before alcoholic fermentation on the oxidative stability of Chardonnay white wines

Sulfites (SO2) addition during winemaking is a widespread practice worldwide. This addition is realized at different steps of the winemaking due to the antimicrobial and antioxidant capacity of SO2. In a context of understanding white wines oxidative stability, knowledge about the impact of SO2 on the wine molecular diversity, especially compounds involved in the antioxidant capacity of wine, appears to be very important. In recent years, some studies have shown that SO2 can react with a large number of wine compounds resulting in the formation of numerous adducts. The diversity of compounds involved is important including in particular pyruvic acid, 2-keto-glutaric acid, glyceraldehyde, sugar, phenolics compounds but also amino acids or peptides. Moreover Roullier-Gall et al. have shown using FT-ICR-MS analysis that the molecular composition of wines remains impacted by addition of SO2 to the must (0, 4 and 8 g/hL SO2), several years after winemaking. Indeed, wines made from protected must (8g/hL SO2) contain a larger diversity of CHOS and CHONS compounds than wines made from unprotected must (0 g/hL SO2). The study of the impact of glutathione addition on the sensory oxidative stability has further shown that CHOS and CHONS compounds (amino acids, aromatic compounds and peptides) are markers of the antioxidant metabolome of white wines. This suggests that CHOS and CHONS compounds arise from SO2 adducts formation but also from a protecting effect of SO2 on the antioxidant metabolome of white wines.

Contribution of very high resolution satellite remote sensing to the mapping of harvest zones in the Maipo Valley (Chile)

Les images de très haute résolution spatiale sont utilisées depuis peu en viticulture comme une aide à la cartographie des zones de vendanges. A partir d’images multispectrales de très haute résolution spatiale IKONOS (résolution 4 m) et SPOT-5 en supermode (résolution 2.5 m), on propose ici une démarche de segmentation d’une région de vignoble en zones de vendanges.

Towards faultless Grenache wines: impact of climate and maturity

Climate change is affecting wine production and inducing significant variability in wine composition between vintages.

NAVIC–20 years of a lean management model for wine business R&D

Considering That Innovation Supports A Company’s Competitive Advantage And Drive Higher Profits (Dogru A. & Peyrette J., 2022), A Key Challenge Of Wine Companies Is Getting Practitioners To Understand That Innovation-Related Wine Research Increases The Likelihood Of Competitive Advantage, Bringing Financial Success. A Continued And Enhanced Investment In Research Is, Thus, A Prerequisite For Commercial Success In Today’s Globalized And Competitive Wine Industry (Høj P., Pretorius I.S., & Day R., 2003).

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.