Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Polyphenols, bioactive secondary metabolites abundantly found in various grapevine components such as stalks, skins, and seeds, have attracted considerable attention in recent decades due to their potential health benefits. These compounds, including flavan-3-ols, flavanols, flavones, and stilbenes, are known for their antioxidant and anti-inflammatory properties.

Aim: Irrigated viticulture is expanding worldwide mainly as a short-term adaptation strategy to climate change. Plant-based methods are increasingly being used for irrigation scheduling in commercial vineyards. Canopy temperature (TC) has long been recognized as an indicator of plant water status. TC, but also the thermal stress indices, e.g. crop water stress index (CWSI) and stomatal

Genetic variants known to produce single residue missense mutations have been associated with phenotypic traits of commercial interest in grapevine. This is the case of the K284N substitution in VviDXS1 associated with muscat aroma, or the R197L in VviAGL11 causing stenospermocarpic seedless grapes. The impact of such mutations on protein structure, stability, dynamics, interactions, or functional mechanism can be studied by computational methods, including our pyDock scoring, previously developed. For this, knowledge on the 3D structure of the protein and its complexes with other proteins and biomolecules is required, but such knowledge is not available for virtually none of the proteins and complexes in grapevine.

1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) is a controversial aroma component found in Riesling wines. It belongs to the family of C13-norisoprenoids and is mainly associated with kerosene/petrol notes. TDN can add complexity to the wine aroma at medium – low concentrations and deteriorate the wine bouquet when its content is high. No TDN aromas are usually perceived in young Riesling wines, but they can appear after several years of aging due to the gradual formation of TDN. Management of TDN in Riesling wines is an actual task, since global warming can promote formation of this compound and compromise the aromatic composition of wine. Therefore, the aim of the current work was, firstly, to study the sensory particularities of TDN in Riesling wine at various concentrations. Secondly, to investigate the ability of bottle closures to absorb (scalp) TDN from Riesling wine under various storage conditions. These studies also include the comparative assessment of our findings with previously published data. METHODS: sensory analysis, GC-MS (SBSE), HPLC,1H-NMR and other methods related to the synthesis and determination of TDN. RESULTS: First of all, the method of the synthesis of highly purified TDN (95% and 99.5%) was optimized [1].

Over the past few decades, viticultural research has made numerous contributions which have made it possible to better understand the behavior of the vine as well as its response to the conditions imposed on it by the environment and agronomic practices. However, these results have only rarely been used in the practical management of vineyards because the research has been carried out using partial experimental models where reality is only represented by a few factors which are sometimes even made more complex by the introduction of elements foreign to the existing situation and difficult to apply to production (varieties, methods of cultivation, management techniques, etc.). To these reasons, one could add a low popularization of the results obtained, as well as the difficulty of implementing the scientific contributions, which does not allow the different production systems to fully express their potential. This limit of viticultural research can only be exceeded by the design of integrated projects designed directly on and for the territory. Indeed, only the integrated evaluation of a viticultural agro-system, which can be achieved through zoning, makes it possible to measure, or even attribute to each element of the system, the weight it exerts on the quality of the wine.