terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Abstract

Downy mildew, caused by the oomycete Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni, is one of the most destructive grapevine diseases mostly affecting Vitis vinifera L. and impacting on viticulture. The pathogen invasion can induce in grapevine multiple defense reactions, first PAMP-Triggered Immunity and secondly Effector-Triggered Immunity. Plasmopara viticola can overcome these defense mechanisms through the secretion of effectors, such as RxLR, into the plant cells, making it easier for the oomycete to infect grapevines.  Currently, the use of chemical pesticides remains the most effective way to control the pathogen with severe negative side effects on the environment and animal health. Consequently, great attention has been recently paid to identifying new Biological Control Agents (BCAs). At CREA Viticulture and Enology, 47 bacterial isolates were collected from the leaves of three Vitis vinifera cultivars, Blush, Dawn seedless and Argentina, showing different degrees of tolerance to Plasmopara viticola. Three of the collected bacteria, that previous tests suggested as potentially good Plasmopara viticolaantagonists, were sprayed on Cabernet sauvignon leaves at 107 CFU ml-1 48 hours before leaf infection with Plasmopara viticola at 106 sporangia ml-1. A gene expression analysis of Plasmopara viticola effectors PvRxLR28 and PvRxLR67, performed through quantitative PCR, revealed an impairment in the expression levels of the two genes in treated leaves compared with control leaves. These results suggest these bacteria as potential BCAs against Plasmopara viticola. Further transcriptomic analysis will be performed to investigate bacterial effects on the expression of multiple Plasmopara viticola pathogenicity genes.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Camilla Mandorino1,2*, Antonella Salerno1,4, Annalisa Prencipe2, Marco Vendemia1, Flavia Angela Maria Maggiolini1, Maria Francesca Cardone1, Andreia Figueiredo3, Antonio Domenico Marsico1, Carlo Pazzani2

1 CREA – Viticulture and Enology, Turi, Italy
2Department of Biosciences, Biotechnology and Environment, University of Bari “A. Moro”, Bari, Italy
3 Biosystems and Integrative Sciences Institute (BioISI), Plant Biology Department, Science Faculty of Lisbon University, Lisbon, Portugal
4 Department of Soil, Plant and Food Science, University of Bari “A. Moro”, Bari, Italy

Contact the author*

Keywords

native vineyard bacteria, biological control agents, Plasmopara viticola, Vitis vinifera, RxLR effectors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Characterizing graft union formation in different scion/rootstock combinations of grapevine 

In most viticultural regions, grapevines are cultivated grafted, employing either hybrid or pure species of various American Vitis spp., such as V. berlandieri, V. rupestris, and V. riparia, as grapevine rootstocks. These rootstocks play a crucial role in providing resistance to the Phylloxera insect pest. Beyond Phylloxera resistance, it is desirable for grapevine rootstocks to exhibit resistance to other soil-borne pathogens and adaptability to abiotic stress conditions. The introduction of new rootstocks holds promise for adapting agriculture to climate change without altering the characteristics of the final harvested product.

Read More

The future of DMS precursors during alcoholic fermentation: impact of yeast assimilable nitrogen levels on the contents of DMSp in young wines

Some red wines develop a “bouquet” during ageing. This complex aroma is linked to quality by wine tasters1. The presence of dimethylsulfide (DMS) in those wines is implicated

Read More

Application of high-throughput sequencing tools for characterisation of microbial communities during alcoholic fermentation

Developments in high-throughput sequencing (HTS) technologies allow us to obtain large amounts of microbial information from wine and must samples. Thus approaches, that are aimed at characterising the microbial diversity during fermentation, can be enhanced, or possibly even replaced, with HTS-based metabarcoding. To reduce experimental biases and increase data reproducibility, we compared 3 DNA extraction methods by evaluating differences in the fungal diversity with Riesling alcoholic fermentation samples at four different vineyards. The fungal diversity profiling was done using the genetic markers ITS2 and D2 using metabarcoding. The extraction methods compared consisted of a commercial kit, a recently published protocol that includes a DNA enhancer, and a protocol based on a buffer containing common inhibitor removal reagents. All methods were able to distinguish vineyard effects on the fungal diversity, but the results differed quantitatively.

Read More

Influence of oak species on the differentiation of aged brandies using chemometrics approach based on phenolic compounds UHPLC fingerprints

Oak is the main material used in cooperage for making barrels and wood chips destined to aged spirits and wines. Quercus alba L., Quercus petraea L. and Quercus robur L. are three of the most commonly used oak species in cooperage companies.

Read More

Pinot blanc: how terroir and pressing techniques impact on the must composition and wine quality

This study investigates how different pressing techniques impact on the sensory profile of Pinot Blanc wines sourced from different terroirs.

Read More