terclim by ICS banner
IVES 9 IVES Conference Series 9 Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Abstract

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed. Three different DNA-based techniques, were used to verify mutations (RAPD, ISSR and SSR markers). We also estimated the ploidy levels of regenerants and differences were observed for 10% of Frappato regenerants that were tetraploid. The different ploidy levels induced several anatomical/morphological changes of the shoots, mature leaves and stomata, which were larger in tetraploid as compared to diploid regenerants. Also, the number of chloroplasts per guard cell pair was higher in tetraploids as compared to diploids; on the contrary, the stomatal index was lower in tetraploids than in diploids. These profound morphological alterations may influence a wide range of physiological processes related to adaptation to environmental stresses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Loredana Abbate1, Caterina Catalano1,2, Antonio Motisi1, Dalila Crucitti1, Francesco Carimi1 and Angela Carra1*

1 Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche Via Ugo La Malfa 153, 90146 Palermo, Italy
2 Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Alfonso Corti 12, 20133 Milan, Italy

Contact the author*

Keywords

autopolyploidy, ploidy variability, somatic embryogenesis,  stomatal characteristics, grapevine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Chemistry and analysis of key volatile compounds of wine and their precursors in grape

A relatively small number of the many volatile substances of wine, often present at trace
concentrations, are considered as key volatile compounds. These compounds often exist in grapes
under poorly odoriferous or non volatile forms as aroma precursors.

Projected changes of grapevine phenology in Belgian and South African vineyards under climate change scenarios

The concept of ‘terroir’ describes the interplay of the environmental factors that affect the grapevine. This includes but is not limited to climate, soil composition, vineyard management, topography, and geology.

PAIRING WINE AND STOPPER: AN OLD ISSUE WITH NEW ACHIEVEMENTS

The sensory characteristics of wine are a topic studied by several researchers over time, but it continues to be a current and challenging subject. These characteristics are fundamental for the consumer acceptability, which has increasingly aroused their interest to modulate them in line with current market trends and innovation demands. The wine physical-chemical and sensory properties depend on a wide set of factors: they begin to be designed in the vineyard and are later constructed during the various stages of winemaking. Afterwards, the wine is placed in bottles and stored or commercialized.

A deep learning object detection approach for smart pest identification in vineyards

Flavescence dorée (FD) poses a significant threat to grapevine health, with the American grapevine leafhopper, Scaphoideus titanus, serving as the primary vector.

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.