terclim by ICS banner
IVES 9 IVES Conference Series 9 Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Abstract

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed. Three different DNA-based techniques, were used to verify mutations (RAPD, ISSR and SSR markers). We also estimated the ploidy levels of regenerants and differences were observed for 10% of Frappato regenerants that were tetraploid. The different ploidy levels induced several anatomical/morphological changes of the shoots, mature leaves and stomata, which were larger in tetraploid as compared to diploid regenerants. Also, the number of chloroplasts per guard cell pair was higher in tetraploids as compared to diploids; on the contrary, the stomatal index was lower in tetraploids than in diploids. These profound morphological alterations may influence a wide range of physiological processes related to adaptation to environmental stresses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Loredana Abbate1, Caterina Catalano1,2, Antonio Motisi1, Dalila Crucitti1, Francesco Carimi1 and Angela Carra1*

1 Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche Via Ugo La Malfa 153, 90146 Palermo, Italy
2 Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Alfonso Corti 12, 20133 Milan, Italy

Contact the author*

Keywords

autopolyploidy, ploidy variability, somatic embryogenesis,  stomatal characteristics, grapevine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A microbial overview of txakoli wine: the case of three appellations of origin

The Txakoli, a white wine produced in the Basque Country (North of Spain), has recently gained popularity due to wine quality improvement and increase in both acreages of production and wine consumption. The aim of this study was to characterize the chemical and microbiological differences between Txakoli wines made with grapes from different sites.

Bioprotective effect of non-Saccharomyces yeasts in wines made without SO2

The sulphur dioxide (SO2) is the most widely used additive in the wine industry because of its preservative action. However, in recent years the number of wineries that produce wines without SO2 has increased significantly because its allergenic character.

Regenerative agricultural winegrowing systems play a role in refining the expression of terroir in the pacific coast region of United States and Canada

By definition, Regenerative Agricultural Systems seek to promote soil and plant health by using photosynthesis for the removal and retention of atmospheric carbon dioxide into stable soil carbon.

Étude de la flore levurienne de différents terroirs alsaciens

L’utilisation de levures sélectionnées est généralement considérée comme le moyen d’éviter les problèmes fermentaires. Néanmoins de nombreux viticulteurs pensent que ces levures sont à l’origine d’une standardisation des vins et militent pour le respect d’une flore indigène (Bourguignon, 1992).

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.