Application of high-throughput sequencing tools for characterisation of microbial communities during alcoholic fermentation

Due to varietal factors, the formation of undesirable deposits of flavonols, especially quercetin (Q), occurs in several red wines.

In a highly competitive worldwide market, a current challenge for the beverage sector is to diversify the range of products and to offer wines and spirits with typicity and character.

During alcoholic fermentation, wine yeasts generate a large variety of volatile metabolites, including acetate esters, ethyl fatty acid esters, higher alcohols, volatile fatty acids and volatile sulfur compounds that contribute to the aroma profile of wine. These molecules, refered as fermentative aromas, are the most abundant volatile compounds synthetized by yeasts and the metabolic pathways involved in their formation have been well characterized. Furthermore, other molecules with a major organoleptic impact may be produced during wine fermentation including terpene derivatives. However, little information is available on the contribution of yeasts to the formation of these molecules, in particular on their ability to synthethise de novo the terpens derivatives or to produce hydrolytic enzymes involved in the release of varietal precursors.

Water tolerance in plants is often associated with the accumulation of osmotic protectants, which are secondary metabolites that can help the plant to cope with water stress. One of the key osmotic protectants is a sugar called Raffinose, which is synthesized by a family of enzymes called Raffinose synthases. In this work, we focused on one of these enzymes, VviRAF2, which is a gene that shows different expression levels and genetic variants (SNPs) among different grapevine cultivars, ranging from tolerant to susceptible to water stress, and the transcription factors that may regulate the expression of this gene family.

Multi-mineral wine profiling and artificial intelligence: implementing the signatures of each wine to train algorithms to meet the new challenges facing the wine industry. Although their quantity is minimal, minerals are essential elements in the composition of every wine. Their presence is the result of complex interactions between factors such as soil, vines, climate, topography, and viticultural practices, all influenced by the terroir. Each stage of the winemaking process also contributes to shaping the unique mineral and taste profile of each wine, giving each cuvée its distinctive characteristics.

The historic Tokaj Wine Region in northeast Hungary, a UNESCO World Heritage region since 2002, encompasses 5,500 ha vineyards. Produced from “noble rot” grapes, Tokaji Aszú is known as one of the oldest botrytized wines all over the world. Special microclimatic conditions (due to Bodrog and Tisza rivers, Indian summer), soil conditions (clay, loess on volcanic bedrock) and grape