OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Grape and wine microorganisms: diversity and adaptation 9 Influence of cell-cell contact on yeast interactions and exo-metabolome

Influence of cell-cell contact on yeast interactions and exo-metabolome

Abstract

Alcoholic fermentation is the main step for winemaking, mainly performed by the yeast Saccharomyces cerevisiae. But other wine yeasts called non-Saccharomyces may contribute to alcoholic fermentation and modulate the wine aroma complexity. The recurrent problem with the use of these non-Saccharomyces yeasts is their trend to die off prematurely during alcoholic fermentation, leading to a lack of their interesting aromatic properties searched in the desired wine. This phenomenon appears to be mainly due to interactions with S. cerevisiae. These interactions are often negatives but remain unclear because of the species and strain specific response. Among the non-Saccharomyces yeasts, Lachancea thermotolerans is a wine yeast naturally found in grape must and well known as a great L-lactic acid producer and an aromatic molecules enhancer, but its behavior during alcoholic fermentation can be completely different in co-fermentation with S. cerevisiae in function of strain used. Thus, S. cerevisiae/L. thermotolerans couple was used to unravel interactions between these two species during alcoholic fermentation. Thanks to a modified S. cerevisiae strain expressing a GFP allowing discrimination between yeast populations, both yeast viability was monitored by flow cytometry in pure and sequential fermentations of grape must with or without cell-cell contact. This reveal a decrease in cell viability for S. cerevisiae in both conditions with a greater decrease in case of cell-cell contact. Investigating the nature of the interactions, our results demonstrate a competition between species for nitrogen compounds, oxygen and for the first time a competition for must sterols. Volatile compounds analysis revealed changes in sequential fermentations compared to pure fermentations and showed also that cell-cell contact modify yeast metabolism since the volatile compound profile was significantly different from sequential fermentation without cell-cell contact. Yeast metabolism modifications associated with cell-cell contact were confirmed further by analyzing the exo-metabolome of all fermentations by FT-ICR-MS analysis. These analyses show for the first time a specific metabolite production and quantitative metabolite changes linked to each fermentation condition. This study shows that cell-cell contact not only impact cell viability as already reported but deeply changes the yeast metabolism.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Clément Petitgonnet, Géraldine L.Klein, Chloé Roullier-Gall, Philippe Schmitt-Kopplin, Beatriz Quintanilla-Casas, Stefania Vichi, Diane Julien-David, Hervé Alexandre

Helmholtz Zentrum Muenchen, Research unit Analytical BioGeoChemistry, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany 
Technische Universität Muenchen, Analytical Food Chemistry, Alte Akademie 10, 85354 Freising, Germany 
Nutrition, Food Science and Gastronomy Department, INSA – XaRTA (Catalonian Reference Network on Food Technology), University of Barcelona, Santa Coloma de Gramenet, Spain 
Université de Strasbourg, CNRS, IPHC UMR 7178, F-67000 Strasbourg, France

Contact the author

Keywords

Interactions, Yeast, Flow cytometry, Exo-metabolome 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Optimization Of Glutathione Extraction From White Wine Lees By Doelhert Matrix

Glutathione (L-g-glutamyl-L-cysteinyl-glycine) is a tripeptide which contains three constitutive amino acids: glutamate, cysteine and glycine. It is present in plants and foods, and fruits like grapes.

Evaluating alternatives to cold stabilization in wineries: the use of carboximethyl cellulose, potassium polyaspartate, electrodialysis and ion exchange resins – the results after one year in the bottle

The tartaric stabilization of wines before bottling to avoid the precipitation of tartaric acid salts is an important and common step during wine production. The presence of precipitated salt crystals in bottle wines is detrimental for their quality and even a legal issue in some countries. Cold stabilization is the most common stabilization treatment. Although it has been shown to be effective, it has some significant disadvantages, mainly regarding losses of color and aromas and its high cost. Therefore, other products and methodologies are being introduced in the wineries for the replacement of this process. Some of these new techniques involve the reduction of the ions causing the insolubilization of tartaric acid while other are based in the formation of protective colloids or the inhibition of the crystallization of salts. In this study, white, rosé and red wines have been treated with carboxymethylcellulose, potassium polyaspartate and an ion exchange resin. The tartaric stability of the wines, together with the oenological, chromatic and sensory characteristics were studied after the wines had been stored during one year in the bottle. The results indicate that the use of carboxymethyl cellulose and potassium polyaspartate maintained the best the sensory and chromatic characteristics and the wine stability of the wines in comparison with an untreated control wine.

Diffuse light due to wildfire smoke enhances gas exchange of shaded leaves

The risk of wildfires is increasing as the frequency and severity of drought and heat waves continue to rise. Wildfires are associated with the combustion of plant materials and emit smoke. In the atmosphere, smoke may spread readily across large areas. Smoke is composed of solid and liquid phase particulates and gases and has been identified as a causal agent of “smoke taint” in wine. On a smoky day, the intensity of direct light decreases because these particulates scatter sunlight. Even though this effect is frequently assumed to decrease plant photosynthesis, this assumption ignores the potential changes in diffuse light and may be based on scant evidence.

Rootstock impact on foliar symptom expression of esca on Vitis vinifera cv. Cabernet-Sauvignon

Trunk diseases and esca in particular, represent a major threat to the sustainability of the vineyards. The percentages of unproductive vines in a plot could vary from 4% to over 20 % depending on local conditions and vintages.

Aptitude du cépage Chenin à l’élaboration de vins liquoreux en relation avec certaines unités terroirs de base de A.O.C. Coteaux du Layon

Massif and the first sedimentary formations of the western aureole of the Paris Basin. If it is found all over the world (California, Israel, South Africa), it is in this region that it best asserts its identity. It is one of the most interesting grape varieties due to the variety and complexity of the wines it can produce. It can give very dry or very sweet, still or sparkling wines, fresh when young and sublime when ageing, expressing the characteristics of each vintage as much as those of the terroir. The Chenin is a faithful witness of its geographical, geological, pedological and climatic environment; he is the foil of the land. It has strong aptitudes for the production of sweet wines conditioned by overripe grapes often botrytised in the AOC Coteaux du Layon.Massif and the first sedimentary formations of the western aureole of the Paris Basin. If it is found all over the world (California, Israel, South Africa), it is in this region that it best asserts its identity. It is one of the most interesting grape varieties due to the variety and complexity of the wines it can produce. It can give very dry or very sweet, still or sparkling wines, fresh when young and sublime when ageing, expressing the characteristics of each vintage as much as those of the terroir. The Chenin is a faithful witness of its geographical, geological, pedological and climatic environment; he is the foil of the land. It has strong aptitudes for the production of sweet wines conditioned by overripe grapes often botrytised in the AOC Coteaux du Layon.