Terroir 2004 banner
IVES 9 IVES Conference Series 9 Flanan-3-ol compositional changes in red grape berries (Vitis vinifera L. cv Cabernet franc) from two terroirs of the Loire Valley (France)

Flanan-3-ol compositional changes in red grape berries (Vitis vinifera L. cv Cabernet franc) from two terroirs of the Loire Valley (France)

Abstract

[English version below]

La quantité et la qualité des flavonoïdes sont des éléments importants de la qualité de la baie. En particulier, les tannins contribuent de manière essentielle aux propriétés spécifiques des vins rouges telles que la couleur, l’astringence et l’amertume. Cependant, leur synthèse et leurs propriétés sont encore mal connues. Ainsi, la compréhension des relations qui existent entre, d’une part, le milieu et d’autre part la mise en place de ce pool tannique jusqu’à la vendange est insuffisante. La composition en tannins des pellicules est suivie depuis le milieu de la phase de croissance herbacée (30 jours après le début floraison) et jusqu’à une maturité normale, sur deux parcelles. L’étude considère un stade donné de développement et non pas la valeur moyenne des paramètres à la parcelle. Ainsi, l’apport d’une relation plus fonctionnelle entre composition et stade physiologique du raisin constitue un levier puissant d’interprétation. La composition en flavan-3-ols et en proanthocyanidines des pellicules est déterminée par HPLC-phase inverse et par histochimie. Le couplage de ces deux techniques permet d’associer des informations quantitatives et spatiales. Cette approche originale permet de mettre en évidence l’importance de la période pré-maturation, en relation avec l’avancement de la maturation mais également d’autres facteurs, comme le nombre de pépins. Qualitativement et quantitativement, les teneurs évoluent peu après véraison. Il est suggéré que le pool tannique est acquis avant véraison. D’autre part, il est montré une relation entre le nombre de pépins des baies et leur qualité.

The quantity and the quality of flavonoïds are important parts of the global quality of the grape berries. Especially, the tannins are responsible of some majors flavour properties of the red wines including colour, bitterness and astringency. Nevertheless, their synthesis and properties are still misunderstood. Thus, the comprehension of the relations between environment and setting of this tannic pool, up to the harvest of the grapes, is not sufficient. The tannin composition was monitored since the middle of the first growth period (30 days after the onset of flowering) to the average maturity, for two plots. The study the stage of the berries, and not the average value of the parameters. Thus, the contribution of a more functional relation between composition and physiological stage of the grape constitutes a powerful lever for interpretation. The composition in flavan-3-ols and in proanthocyanidins of skins were determined by HPLC reversed phase and by histochemistry. The linking of these two analytical techniques allowed the association of quantitative and spatial data. This original approach pointed out the importance of the period previous maturation in relation with the stage of maturation but also others factors, such as the number of seeds. There was little evolution of the tannins after veraison. It is suggested that the tannic pool is set before veraison. More, a relation between the number of seeds and the quality of the berries was shown.

DOI:

Publication date: January 12, 2022

Issue: Terroir 2004

Type: Article

Authors

Y. Cadot (1), M.T. Miňana (1), R. Champenois (1), M. Chevalier (2) and G. Barbeau (1)

(1) Unité Vigne et Vin, Institut National de la Recherche Agronomique, Centre de Recherches d’Angers, 42 rue Georges Morel, 49071 Beaucouzé Cedex, France
(2) Unité Mixte de Recherche Génétique et Horticulture (GenHort), Institut National de la Recherche Agronomique, Centre de Recherches d’Angers, 42 rue Georges Morel, 49071 Beaucouzé Cedex, France

Contact the author

Keywords

Tannins, maturation, Vitis vinifera var. cabernet franc, skin, seed, histochemistry

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Photoselective shade films affect grapevine berry secondary metabolism and wine composition

Grapevine physiology and production are challenged by forecasted increases in temperature and water deficits. Within this scenario, photoselective overhead shade films are promising tools in warm viticulture areas to overcome climate change related factors. The aim of this study was to evaluate the vulnerability of ‘Cabernet Sauvignon’ grape berry to solar radiation overexposure and optimize shade film use for berry integrity. A randomized complete block design field study was conducted across two years (2020-2021) in Oakville, Napa Valley, CA, with four shade films (D1, D3, D4, D5) differing in the percent of radiation spectra transmitted and compared to an uncovered control (C0). Integrals for gas exchange parameters and mid-day stem water potential were unaffected by the shade films in 2020 and 2021. By harvest, berries from uncovered and shaded vines did not differ in their size or primary metabolism in either year. Despite precipitation exclusion during the dormant season in the shaded treatments, yield did not differ between them and the control in either season. In 2020, total skin anthocyanins (mg/g fresh mass) in the shaded treatments was greater than C0 during berry ripening and at harvest. Conversely, flavonol concentrations in 2020 were reduced in shaded vines compared to C0. The 2020 growing season highlighted the impact of heat degradation on flavonoids. Flavonoid concentrations in 2021 increased until harvest while flavonoid degradation was apparent from veraison to harvest in 2020 across shaded and control vines. Wine analyses highlighted the importance of light spectra to modify wine composition. Wine color intensity, tonality and anthocyanin values were enhanced in D4 whereas antioxidant properties were enhanced in C0 and D5 wines. Altogether, our results highlighted the need of new approaches in warm viticulture areas given the impact that composition of light has on berry and wine quality.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.

Updating the Winkler index: An analysis of Cabernet sauvignon in Napa Valley’s varied and changing climate

This study aims to create an updated, agile viticultural climate index (similar to the Winkler Index) by performing in-depth analyses of current and historical data from industry partners in several major winegrowing regions. The Winkler Index was developed in the early twentieth century based on analysis of various grape-growing regions in California. The index uses heat accumulation (i.e. Growing Degree Days) throughout the growing season to determine which grape varieties are best suited to each region. As viticultural regions are increasingly subject to the complexity and uncertainty of a changing climate, a more rigorous, agile model is needed to aid grape growers in determining which cultivars to plant where. For the first phase of this study, 21 industry partners throughout Napa Valley shared historical phenology, harvest, viticultural practice, and weather data related to their Cabernet sauvignon vineyard blocks. To complement this data, berry samples were collected throughout the 2021 growing season from 50 vineyard blocks located throughout 16 American Viticultural Areas that were then analyzed for basic berry chemistry and phenolics. These blocks have been mapped using a Geographic Information System (GIS), enabling analysis of altitude, vineyard row orientation, slope, and remotely sensed climate data. Sampling sites were also chosen based on their proximity to a weather station. By analyzing historical data from industry partners and data specifically collected for this study, it is possible to identify key parameters for further analysis. Initial results indicate extreme variability at a high spatial resolution not currently accounted for in modern viticultural climate indices and suggest that viticultural practices play a major role. Using the structure of data collection and analyses developed for the first phase, this project will soon be expanded to other wine regions globally, while continuing data collection in Napa Valley.

Water deficit differentially impacts the performances and the accumulation of grape metabolites of new varieties tolerant to fungi

The use of resistant varieties is a long-term but promising solution to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are now releasing a range of new hybrids performing well regarding fungi susceptibility and producing good quality wines. Unfortunately, insufficient attention is paid by the breeders to the adaptation of these varieties to climatic changes, notably to the increased climatic demand and water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD. This study aimed to characterize the different drought-strategies adopted by 6 new resistant varieties selected by INRAE in comparison to Syrah. To allow the assessment of long-term impacts of WD, field-grown vines were exposed to contrasted WD from 2018 to 2021 under a semi-arid Mediterranean climate. A gradient of WD was applied in the field and controlled through plant measurements at the single plant level. Grape development was non-destructively monitored to determine the arrest of berry phloem unloading. The impacts of WD on berry composition, including water, primary metabolites (sugars, organic acids), secondary metabolites (anthocyanins, thiols precursors) and main cations contents, were assessed at this specific stage. Results showed different varietal responses during the year and inter-annual acclimation in terms of plant water use efficiency, biomass accumulation, as well as yield components and berry composition. WD differentially reduced the accumulation of primary metabolites at plant and berry levels, but it little changed their concentrations in the fruits at the ripe stage. Moreover, WD differentially impacted the accumulation of secondary metabolites and major cations between the varieties. In the talk, we’ll present the main results regarding the WD impacts on fruit metabolites and enlarge the reflection about the practical assessment of the grapevine acclimation to WD.

Mobile device to induce heat-stress on grapevine berries

Studying heat stress response of grapevine berries in the field often relies on weather conditions during the growing season. We constructed a mobile heating device, able to induce controlled heat stress on grapes in vineyards. The heater consisted of six 150 W infrared lamps mounted in a profile frame. Heating power of the lamps could be controlled individually by a control unit consisting of a single board computer and six temperature sensors to reach a pre-set temperature. The heat energy applied to individual berries within a cluster decreases by the squared distance to the heat source, enabling the establishment of temperature profiles within individual clusters. These profiles can be measured by infrared thermography once a steady state has been reached. Radiant flux density received by a berry depending on the distance was calculated based on a view factor and measured lamp surface temperature and resulted to 665 Wm-2 at 7cm. Infrared thermography of the fruit surface was in good agreement with measurements conducted with a thermocouple inserted at epidermis level. In combination with infrared thermography, the presented device offers possibilities for a wide range of applications like phenotyping for heat tolerance in the field to proceed in the understanding of the complex response of plants to heat stress. Sunburn necrosis symptoms were artificially induced with the aid of the device for cv. Bacchus and cv. Sylvaner in the 2020 and 2021 growing season. Threshold temperatures for sunburn induction (LT5030min) were derived from temperature data of single berries and visual sunburn assessment, applying logistic regression. A comparison of threshold temperatures for the occurrence of sunburn necrosis confirmed the higher susceptibility of cv. Bacchus. The lower susceptibility of cv. Sylvaner did not seem to be related to its phenolic composition, rendering a thermoprotective role of berry phenolic compounds unlikely.