Terroir 2004 banner
IVES 9 IVES Conference Series 9 Flanan-3-ol compositional changes in red grape berries (Vitis vinifera L. cv Cabernet franc) from two terroirs of the Loire Valley (France)

Flanan-3-ol compositional changes in red grape berries (Vitis vinifera L. cv Cabernet franc) from two terroirs of the Loire Valley (France)

Abstract

[English version below]

La quantité et la qualité des flavonoïdes sont des éléments importants de la qualité de la baie. En particulier, les tannins contribuent de manière essentielle aux propriétés spécifiques des vins rouges telles que la couleur, l’astringence et l’amertume. Cependant, leur synthèse et leurs propriétés sont encore mal connues. Ainsi, la compréhension des relations qui existent entre, d’une part, le milieu et d’autre part la mise en place de ce pool tannique jusqu’à la vendange est insuffisante. La composition en tannins des pellicules est suivie depuis le milieu de la phase de croissance herbacée (30 jours après le début floraison) et jusqu’à une maturité normale, sur deux parcelles. L’étude considère un stade donné de développement et non pas la valeur moyenne des paramètres à la parcelle. Ainsi, l’apport d’une relation plus fonctionnelle entre composition et stade physiologique du raisin constitue un levier puissant d’interprétation. La composition en flavan-3-ols et en proanthocyanidines des pellicules est déterminée par HPLC-phase inverse et par histochimie. Le couplage de ces deux techniques permet d’associer des informations quantitatives et spatiales. Cette approche originale permet de mettre en évidence l’importance de la période pré-maturation, en relation avec l’avancement de la maturation mais également d’autres facteurs, comme le nombre de pépins. Qualitativement et quantitativement, les teneurs évoluent peu après véraison. Il est suggéré que le pool tannique est acquis avant véraison. D’autre part, il est montré une relation entre le nombre de pépins des baies et leur qualité.

The quantity and the quality of flavonoïds are important parts of the global quality of the grape berries. Especially, the tannins are responsible of some majors flavour properties of the red wines including colour, bitterness and astringency. Nevertheless, their synthesis and properties are still misunderstood. Thus, the comprehension of the relations between environment and setting of this tannic pool, up to the harvest of the grapes, is not sufficient. The tannin composition was monitored since the middle of the first growth period (30 days after the onset of flowering) to the average maturity, for two plots. The study the stage of the berries, and not the average value of the parameters. Thus, the contribution of a more functional relation between composition and physiological stage of the grape constitutes a powerful lever for interpretation. The composition in flavan-3-ols and in proanthocyanidins of skins were determined by HPLC reversed phase and by histochemistry. The linking of these two analytical techniques allowed the association of quantitative and spatial data. This original approach pointed out the importance of the period previous maturation in relation with the stage of maturation but also others factors, such as the number of seeds. There was little evolution of the tannins after veraison. It is suggested that the tannic pool is set before veraison. More, a relation between the number of seeds and the quality of the berries was shown.

DOI:

Publication date: January 12, 2022

Issue: Terroir 2004

Type: Article

Authors

Y. Cadot (1), M.T. Miňana (1), R. Champenois (1), M. Chevalier (2) and G. Barbeau (1)

(1) Unité Vigne et Vin, Institut National de la Recherche Agronomique, Centre de Recherches d’Angers, 42 rue Georges Morel, 49071 Beaucouzé Cedex, France
(2) Unité Mixte de Recherche Génétique et Horticulture (GenHort), Institut National de la Recherche Agronomique, Centre de Recherches d’Angers, 42 rue Georges Morel, 49071 Beaucouzé Cedex, France

Contact the author

Keywords

Tannins, maturation, Vitis vinifera var. cabernet franc, skin, seed, histochemistry

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Grape must quality and mesoclimatic variability in Fruška Gora wine-growing region, Serbia

The Fruška Gora mountain is a traditional wine-growing region in Serbia situated in the Pannonian Basin. Due to such a position, the vicinity of the Danube River and the presence of concave configuration, it is suitable for grape production. This paper provides analyses of spatial variations in meteorological parameters and grape juice quality within Fruška Gora wine region over three consecutive vintages (2018-2020). The examined period can be defined as warm with cool nights during September (AVG 18,9°C; GDD 1918°C; CI 12°CF) and with the presence of mesoclimatic variability. The East part of the study area was somewhat drier and hotter compared to other parts of the region. The analyses of grape must samples (190 in total) of five cultivars (Cabernet-Sauvignon, Merlot, Chardonnay, Sauvignon blanc and Grašac (Welschriesling)) commonly grown across the region (19 sites), were performed using Fourier Transform Infrared Technology (FTIR). Among all cultivars, Sauvignon blanc was harvested first in the East area (DOY=246±5, GDD at harvest=1552±74, 22.2±0.7 °Brix), while the latest harvest was recorded for Cabernet-Sauvignon in the West (DOY=283±5, GDD at harvest=1936±187, 23.4±1.0 °Brix ). Both the red and white cultivars had higher acidity and YAN in the grape must if the vines were grown in the North and East compared to South and West areas. According to PCA analysis, Grašac showed the lowest variation in grape must chemical composition. Thus, the results confirm that Grašac is the most stable cultivar in Fruška Gora. All monitored cultivars reached technological fruit ripeness by the end of the growing season. However, it was difficult to reach full ripeness of red cultivars, mostly beacuse of uncoupling of technolocical and phenolic ripeness. Thus, Cabernet-Sauvignon had higher variations in GDD sums at harvest compared to other cultivars, which probably increased variations in grape must quality.

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Photoselective shade films affect grapevine berry secondary metabolism and wine composition

Grapevine physiology and production are challenged by forecasted increases in temperature and water deficits. Within this scenario, photoselective overhead shade films are promising tools in warm viticulture areas to overcome climate change related factors. The aim of this study was to evaluate the vulnerability of ‘Cabernet Sauvignon’ grape berry to solar radiation overexposure and optimize shade film use for berry integrity. A randomized complete block design field study was conducted across two years (2020-2021) in Oakville, Napa Valley, CA, with four shade films (D1, D3, D4, D5) differing in the percent of radiation spectra transmitted and compared to an uncovered control (C0). Integrals for gas exchange parameters and mid-day stem water potential were unaffected by the shade films in 2020 and 2021. By harvest, berries from uncovered and shaded vines did not differ in their size or primary metabolism in either year. Despite precipitation exclusion during the dormant season in the shaded treatments, yield did not differ between them and the control in either season. In 2020, total skin anthocyanins (mg/g fresh mass) in the shaded treatments was greater than C0 during berry ripening and at harvest. Conversely, flavonol concentrations in 2020 were reduced in shaded vines compared to C0. The 2020 growing season highlighted the impact of heat degradation on flavonoids. Flavonoid concentrations in 2021 increased until harvest while flavonoid degradation was apparent from veraison to harvest in 2020 across shaded and control vines. Wine analyses highlighted the importance of light spectra to modify wine composition. Wine color intensity, tonality and anthocyanin values were enhanced in D4 whereas antioxidant properties were enhanced in C0 and D5 wines. Altogether, our results highlighted the need of new approaches in warm viticulture areas given the impact that composition of light has on berry and wine quality.

Differential responses of red and white grape cultivars trained to a single trellis system – the VSP

Commercial grape production relies on training grapevine cultivars onto a variety of trellis systems. Training allows for well-lit leaves and clusters, maximizing fruit quality in addition to facilitating cultivation, harvesting, and diseases control. Although grapevines can be trained onto an infinite variety of trellis systems, most red and white cultivars are trained to the standard VSP (Vertical Shoot Positioning) system. However, red and white cultivars respond differently to VSP in fruit composition and growth characteristics, which are yet to be fully understood. Therefore, the objective of this study was to examine the influence of the VSP trellis system on fruit composition of three red, Cabernet Sauvignon, Merlot and Syrah, and three white, Chardonnay, Riesling, and Gewurztraminer cultivars grown under uniform growing conditions in the same vineyard. All cultivars were monitored for maturity and harvested at their physiologically maximum possible sugar concentration to compare various fruit quality attributes such as Brix, pH, TA, malic and tartaric acids, glucose and fructose, potassium, YAN, and phenolic compounds including total anthocyanins, anthocyanin profile, and tannins. A distinct pattern in fruit composition was observed in each cultivar. In regards to growth characteristics, Syrah grew vigorously with the highest cluster weight. Although all cultivars developed pyriform seeds, the seed size and weight varied among all cultivars. Also varied were mesocarp cell viability, brush morphology, and cane structure. This knowledge of the canopy architectural characteristics assessed by the widely employed fruit compositional attributes and growth characteristics will aid the growers in better management of the vines in varied situations.