Terroir 2004 banner
IVES 9 IVES Conference Series 9 Contribution of phenolic compounds to the total antioxidant capacity of Pinotage wine

Contribution of phenolic compounds to the total antioxidant capacity of Pinotage wine

Abstract

The South African wine industry is taking an interest in the enhancement of red wine total antioxidant capacity (TAC) with retention of sensory quality to satisfy the demands of increasingly discerning consumers. The focus is especially on the unique South African red wine cultivar, Pinotage. Pinotage has a unique phenolic composition and commercial Pinotage wines (1998 vintage) has an average TAC of 15.3 mM Trolox equivalents which compares well with that of Cabernet Sauvignon. Knowledge of wine phenolic composition, the antioxidant activity of individual phenolic compounds and their respective contribution to the TAC of wines are needed to evaluate the importance of individual phenolic compounds. The TAC of wines could then be manipulated optimally by using viticultural and enological practices to enhance the content of compounds contributing significantly to the TAC. The aim of the study was to determine the antioxidant activity of individual phenolic compounds in Pinotage wines and their contribution to TAC.
A series of 20 young Pinotage wines were analysed to determine their phenolic composition (reversed-phase HPLC) and TAC (ABTS radical cation scavenging assay). Compounds identified include gallic acid, caftaric acid, caffeic acid, coutaric acid, catechin, procyanidin B1, myricetin-3-glucoside (glc), quercetin-3-glc, kaempferol-3-glc, quercetin-3-rhamnoside, myricetin, quercetin, kaempferol, isorhamnetin, delphinidin-3-glc, peonidin-3-glc, petunidin-3-glc, malvidin-3-glc, delphinidin-3-glc-acetate, vitisinA, petunidin-3-glc-acetate, peonidin-3-glc-acetate, malvidin-3-glc-acetate and malvidin-3-glc-coumarate. The polymeric content of each wine was also estimated as mg catechin equivalents/L. Individual phenolic compounds, available as pure standards (gallic acid, caffeic acid, catechin, procyanidin B1, myricetin-3-glc, quercetin-3-glc, kaempferol-3-glc, quercetin-3-rhamnoside, myricetin, quercetin, kaempferol, isorhamnetin, delphinidin-3-glc, peonidin-3-glc, petunidin-3-glc, malvidin-3-glc), were tested at a range of concentrations and their Trolox equivalent antioxidant capacity (TEAC) values calculated.
Taking the concentration and TEAC values of 24 monomeric phenolic compounds which could be quantified, into account, only 14% of the TAC of the wines could be explained. Possible synergism was ruled out, as the measured and calculated TAC of a mixture of phenolic standards was within the experimental error. Sulphur dioxide additions to the phenolic mixtures at two concentrations had no effect on their TAC. To estimate the contribution of polymeric compounds ultrafiltration was performed in an attempt to separate monomers and polymers in 3 wines. The polymeric compounds, and possibly proteins, isolated using ultrafiltration (50000 dalton nominal molecular weight cut-off), contribute about 30% of their TAC values. A large fraction (59%) of the TAC of a wine is due to unknown compounds which may or may not be phenolic.

DOI:

Publication date: January 12, 2022

Issue: Terroir 2004

Type: Article

Authors

Dalene de Beer (1), Elizabeth Joubert (2), Johann Marais (2), Marena Manley (1)

(1) Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa
(2) Post-Harvest and Wine Technology, ARC Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa

Contact the author

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Assessing the climate change vulnerability of European winegrowing regions by combining exposure, sensitivity and adaptive capacity indicators

Winegrowing regions recognized as protected designations of origin (PDOs) are closely tied to well defined geographic locations with a specific set of pedoclimatic attributes and strictly regulated by legal specifications. However, climate change is increasingly threatening these regions by changing local conditions and altering winegrowing processes. The vulnerability to these changes is largely heterogenous across different winegrowing regions because it is determined by individual characteristics of each region, including the capacity to adapt to new climatic conditions and the sensitivity to climate change, which depend not only on natural, but also socioeconomic and legal factors. Accurate vulnerability assessments therefore need to combine information about adaptive capacity and climate change sensitivity with projected exposure to new climatic conditions. However, most existing studies focus on specific impacts neglecting important interactions between the different factors that determine climate change vulnerability. Here, we present the first comprehensive vulnerability assessment of European wine PDOs that spatially combines multiple indicators of adaptive capacity and climate change sensitivity with high-resolution climate projections. We found that the climate change vulnerability of PDO areas largely depends on the complex interactions between physical and socioeconomic factors. Homogenous topographic conditions and a narrow varietal spectrum increase climate change vulnerability, while the skills and education of farmers, together with a good economic situation, decrease their vulnerability. Assessments of climate change consequences therefore need to consider multiple variables as well as their interrelations to provide a comprehensive understanding of the expected impacts of climate change on European PDOs. Our results provide the first vulnerability assessment for European winegrowing regions at high spatiotemporal resolution that includes multiple factors related to climate exposure, sensitivity, and adaptive capacity on the level of single winegrowing regions. They will therefore help to identify hot spots of climate change vulnerability among European PDOs and efficiently direct adaptation strategies.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.

The combined effects of climate, soils, and deficit irrigation on yield and quality of Touriga Nacional under high atmospheric demand in the Douro Region

Global warming is one of the biggest environmental, social and economic threats in several viticultural regions. In the Douro Valley, changes are expected in the coming years, namely an increase in temperature and a decrease in precipitation. These changes are likely to have consequences for the production and quality of wine.
The aim of this study was to explore the effects of different soil characteristics combined with several deficit irrigation strategies, managed throughout ETc references and predawn leaf water potentials thresholds, on physiology, yield, and qualitative attributes on the Touriga Nacional variety under years of mild to severe water and heat stress.
The studies were conducted over seven years (2015 to 2021) in two plots of a commercial vineyard located at Quinta do Ataíde (Symington Family Estates) planted in 2011 and 2014 at 170 meters elevation, growing under three water regimes: non-irrigated (NI) and two deficit irrigation strategies (30% and 60% ETc) assessed weekly by Ψpd. The site has an annual rainfall below 500 mm, with high atmospheric demand. Climate data was collected from a weather station, located on site. Berry ripening was followed weekly for fruit analysis. At harvest, yield, vigour and pruning weight per vine were determined from 90 vines by treatment. Each season at veraison the NDVI Index was accessed by a drone. The soils physic-chemistry in the experimental blocs were analysed and grouped by SWHC. Delta C-13 analyses were also performed per treatment in two years.Irrigation had a positive effect on yield per vine, mostly due to an increase in berry and cluster weight, and fertility index through the years. A significant increase in sugar content, colour and phenols was observed with deficit irrigation in some years, but vine vigour related to soil characteristics had by far the greatest impact on quality.