Terroir 2004 banner
IVES 9 IVES Conference Series 9 Contribution of phenolic compounds to the total antioxidant capacity of Pinotage wine

Contribution of phenolic compounds to the total antioxidant capacity of Pinotage wine

Abstract

The South African wine industry is taking an interest in the enhancement of red wine total antioxidant capacity (TAC) with retention of sensory quality to satisfy the demands of increasingly discerning consumers. The focus is especially on the unique South African red wine cultivar, Pinotage. Pinotage has a unique phenolic composition and commercial Pinotage wines (1998 vintage) has an average TAC of 15.3 mM Trolox equivalents which compares well with that of Cabernet Sauvignon. Knowledge of wine phenolic composition, the antioxidant activity of individual phenolic compounds and their respective contribution to the TAC of wines are needed to evaluate the importance of individual phenolic compounds. The TAC of wines could then be manipulated optimally by using viticultural and enological practices to enhance the content of compounds contributing significantly to the TAC. The aim of the study was to determine the antioxidant activity of individual phenolic compounds in Pinotage wines and their contribution to TAC.
A series of 20 young Pinotage wines were analysed to determine their phenolic composition (reversed-phase HPLC) and TAC (ABTS radical cation scavenging assay). Compounds identified include gallic acid, caftaric acid, caffeic acid, coutaric acid, catechin, procyanidin B1, myricetin-3-glucoside (glc), quercetin-3-glc, kaempferol-3-glc, quercetin-3-rhamnoside, myricetin, quercetin, kaempferol, isorhamnetin, delphinidin-3-glc, peonidin-3-glc, petunidin-3-glc, malvidin-3-glc, delphinidin-3-glc-acetate, vitisinA, petunidin-3-glc-acetate, peonidin-3-glc-acetate, malvidin-3-glc-acetate and malvidin-3-glc-coumarate. The polymeric content of each wine was also estimated as mg catechin equivalents/L. Individual phenolic compounds, available as pure standards (gallic acid, caffeic acid, catechin, procyanidin B1, myricetin-3-glc, quercetin-3-glc, kaempferol-3-glc, quercetin-3-rhamnoside, myricetin, quercetin, kaempferol, isorhamnetin, delphinidin-3-glc, peonidin-3-glc, petunidin-3-glc, malvidin-3-glc), were tested at a range of concentrations and their Trolox equivalent antioxidant capacity (TEAC) values calculated.
Taking the concentration and TEAC values of 24 monomeric phenolic compounds which could be quantified, into account, only 14% of the TAC of the wines could be explained. Possible synergism was ruled out, as the measured and calculated TAC of a mixture of phenolic standards was within the experimental error. Sulphur dioxide additions to the phenolic mixtures at two concentrations had no effect on their TAC. To estimate the contribution of polymeric compounds ultrafiltration was performed in an attempt to separate monomers and polymers in 3 wines. The polymeric compounds, and possibly proteins, isolated using ultrafiltration (50000 dalton nominal molecular weight cut-off), contribute about 30% of their TAC values. A large fraction (59%) of the TAC of a wine is due to unknown compounds which may or may not be phenolic.

DOI:

Publication date: January 12, 2022

Issue: Terroir 2004

Type: Article

Authors

Dalene de Beer (1), Elizabeth Joubert (2), Johann Marais (2), Marena Manley (1)

(1) Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa
(2) Post-Harvest and Wine Technology, ARC Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa

Contact the author

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Local adaptation tools to ensure the viticultural sustainability in a changing climate

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.

Climate projections over France wine-growing region and its potential impact on phenology

Climate change represents a major challenge for the French wine industry. Climatic conditions in French vineyards have already changed and will continue to evolve. One of the notable effects on grapevine is the advancing growing season. The aim of this study is to characterise the evolution of agroclimatic indicators (Huglin index, number of hot days, mean temperature, cumulative rainfall and number of rainy days during the growing season) at French wine-growing regions scale between 1980 and 2019 using gridded data (8 km resolution, SAFRAN) and for the middle of the 21th century (2046-2065) with 21 GCMs statistically debiased and downscaled at 8 km. A set of three phenological models were used to simulate the budburst (BRIN, Smoothed-Utah), flowering, veraison and theoretical maturity (GFV and GSR) stages for two grape varieties (Chardonnay and Cabernet-Sauvignon) over the whole period studied. All the French wine-growing regions show an increase in both temperatures during the growing season and Huglin index. This increase is accompanied by an advance in the simulated flowering (+3 to +9 days), veraison (+6 to +13 days) and theoretical maturity (+6 to +16 days) stages, which are more noticeable in the north-eastern part of France. The climate projections unanimously show, for all the GCMs considered, a clear increase in the Huglin index (+662 to 771 °C.days compared to the 1980-1999 period) and in the number of hot days (+5.6 to 22.6 days) in all the wine regions studied. Regarding rainfall, the expected evolution remains very uncertain due to the heterogeneity of the climates simulated by the 21 models. Only 4 regions out of 21 have a significant decrease in the number of rainy days during the growing season. The two budburst models show a strong divergence in the evolution of this stage with an average difference of 18 days between the two models on all grapevine regions. The theoretical maturity is the most impacted stage with a potential advance between 40 and 23 days according to wine-growing regions.

VINIoT – Precision viticulture service

The project VINIoT pursues the creation of a new technological vineyard monitoring service, which will allow companies in the wine sector in the SUDOE space to monitor plantations in real time and remotely at various levels of precision. The system is based on spectral images and an IoT architecture that allows assessing parameters of interest viticulture and the collection of data at a precise scale (level of grape, plant, plot or vineyard) will be designed. In France, three subjects were specifically developed: evaluation of maturity, of water stress, and detection of flavescence dorée. For the evaluation of maturity, it has been decided first to work at the berry scale in the laboratory, then at the bunch scale and finally in the vineyard. The acquisition of the spectral hyperstal image as well as the reference analyzes to measure the maturity, were carried out in the laboratory after harvesting the berries in a maturity monitoring context. This work focuses on a case study to predict sugar content of three different grape varieties: Syrah, Fer Servadou and Mauzac. A robust method called Roboost-PLSR, developed in the framework of this work (Courand et al., 2022), to improve prediction model performance was applied on spectra after the acquirement of hyperspectral images. Regarding the evaluation of water stress, to work with a significant variability in terms of water status, it has been worked first with potted plants under 2 different water regimes. The facilities have allowed the supervision of irrigation and micro-climatic conditions. The regression models on agronomic variables (stomatal conductance, water potential, …) are studied. To detect flavescence dorée, the experimental plan has consisted of work at leaf scale in the laboratory first, and then in the field. To detect the disease from hyper-spectral imaging, a combination of multivariate curve resolution-alternating least squares (MCR-ALS) and factorial discriminant analysis (FDA) was proposed. This strategy proved the potential towards the discrimination of healthy and infected leaves by flavescence dorée based on the use of hyperspectral images (Mas Garcia et al., 2021).