Leaf vine content in nutrients and trace elements in La Mancha (Spain) soils: influence of the rootstock

The content of this communication arises from the deliberations of a working group mandated within the framework of the INRA-INAO 2000-2003 research convention, which brought together INAO representatives and researchers who had worked on AOCs or PGIs, in disciplines from the sphere of the humanities (consumer science, marketing, rural development) and biotechnical sciences (agronomy, animal production science, technology, biochemistry).

Non-targeted analysis is applied in many different domains of analytical chemistry such as metabolomics, environmental and food analysis. In contrast to targeted analysis, non-targeted approaches take information of known and unknown compounds into account, are inherently more comprehensive and give a more holistic representation of the sample composition.

Context and purpose of the study. As other perennial crops grapevine is facing the challenges of climate changes. One of the major issues is global warming and variations of the water budget.

The vineyard has experienced a general increase in yields mainly due to the elevated use of technology which caused a quality loss of grapes in more than one case. A large percentage of the Spanish vineyard is covered by a Denomination of Origin which limits the productive level of the vineyards as one of its regulations. The maximum production limit is a variable characteristic of each vineyard and is not usually regulated by agronomic criteria, and this explains the fact that each vineyard can reach high quality with a totally different yield from that set by the Denomination of Origin.

The undergoing global warming scenario is affecting grapevines phenology, including the timing of berry ripening and harvest date, negatively impacting production and quality. This work reports the crucial role of NAC61, a grapevine NAC transcription factor, in regulating metabolic processes occurring from the onset of ripening onwards. NAC61 high confidence targets mainly represent genes acting on stilbene biosynthesis and regulation, and in osmotic and oxidative/biotic stress-related responses. The direct regulation of the stilbene synthase regulator MYB14, the osmotic stress-related gene DHN1b, and the Botrytis cinerea susceptibility gene WRKY52, were all further validated.