WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Mining terroir influence on bioactive polyphenols from grape stems: A correlation-network-driven approach to spatialize metabolomics data

Mining terroir influence on bioactive polyphenols from grape stems: A correlation-network-driven approach to spatialize metabolomics data

Abstract

In viticulture, the concept of terroir is often used to enlighten the environmental-based typicity of grapevines grown in a local area however its scientific basis remains under debate. Grape polyphenols as key player of the plant defense system enables adaptation to environmental changes and so far, form a unique metabolic component to investigate the terroir influence. Using correlation-based networks and spatial metabolomics we investigated how continuous changes of soil properties may impact the polyphenol composition within a single grape clone. In a vineyard parcel covering four different geological layers and planted with a unique clone of Cabernet Franc, the soil texture was analyzed at 30 georeferenced points with a spatial coverage sampling strategy. Grape stems were harvested at corresponding positions over 3 consecutive years followed by UPLC-DAD-MS-based metabolomic analysis targeted on 43 metabolites including flavonoids, phenolic acids, procyanidins and stilbenoids. Principal component analyses on intra-vintage data presented good reproducibility. A correlation-driven approach was used to select co-varying metabolites before using Geographic Information System (GIS). As results, flavonoids and stilbenoid DP4 were spatialized according to soil granulometry, with stilbenoid DP4 over-accumulating in loamy-silty soils and flavonoids in sandy soils. The present study highlights soil-based terroir influence on polyphenols in a continuous space. Spatial metabolomics driven by correlation-based networks represents a powerful approach to spatialize field-omics data and may serve as new field-phenotyping tool in precision agriculture.

DOI:

Publication date: June 9, 2022

Issue: WAC 2022

Type: Article

Authors

Arnaud Lanoue, Kévin Billet, , Sébastien Salvador-Blanes, Thomas Dugé de Bernonville, Guillaume Delanoue, Florent Hinschberger, Audrey Oudin, Vincent Courdavault, Olivier Pichon, Sébastien Besseau, Samuel Leturcq, Nathalie Giglioli-Guivarc’h, Arnaud Lanoue

Presenting author

Arnaud Lanoue – EA 2106 Biomolécules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours

Institut Français de la Vigne et du Vin, Tours | GéoHydrosystèmes Continentaux (GéHCO), EA 6293, Université de Tours | Laboratoire CITERES, Equipe Laboratoire Archéologie et Territoires (LAT), UMR 7324 CNRS, Université de Tours| EA 2106 Biomolécules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours

Contact the author

Keywords

Terroir – metabolomics – grape polyphenols – Geographic Information System – correlation network

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

The effect of viticultural treatment on grape juice chemical composition

Viticultural management regimes influence the soil elemental profile of a vineyard, determining the microbial community distribution, insect life, and plant biochemistry and physiology

Read More

Ancient and recent construction of Terroirs

The local wine as an area identified and recognized is a complex socio-historical reality that calls an effort of observation and theoretical reflection using various social sciences

Read More

A comprehensive study on the effect of foliar mineral treatments on grapevine microbiota, flavonoid gene expression, and berry composition

Recently, foliar treatments with mineral-based compounds have shown positive effects on grapevine production by protecting grape from thermal excesses and reducing the decoupling between technological and phenolic maturity caused by climate change. Unraveling the effect of mineral particle applications on grape-associated microbes is pivotal for successful wine processing, due to the influence of the microbiota on wine composition and stability. To our knowledge, this is the first work that comprehensively studied the effects of kaolin and chabasite-rich zeolitites treatments on grape-related microorganisms (by real-time PCR quantification of total fungi, Hanseniospora uvarum, Metschnikowia pulcherrima, plant-associated bacteria and lactic acid bacteria), the expression of genes related to the flavonoid biosynthesis (PAL1, CHS1, F3H2, DFR, LDOX, UFGT, MYBA1, GST4, FLS4 genes) and the berry composition (°Brix, pH, acidity and anthocyanin concentrations) in cv. Sangiovese during ripening in two growing seasons (2019 and 2020).

Read More

The effect of wine cork closures on volatile sulfur compounds during accelerated post-bottle ageing in Shiraz wines

Reduced off-flavour is an organoleptic defect due to an excess of volatile sulfur compounds (VSC) in wine and often happening in Shiraz wines. This off-flavour is a direct consequence of the lack of oxygen flow during winemaking and bottle storage. Therefore, wine closure could have a direct impact on the formation of VSC due to the oxygen transfer rate that can modulate their levels. Even if dimethylsulfide (DMS) contributes to reduced off-flavor, it is also a fruity note enhancer in wine and its evolution during wine ageing is not well understood.

Read More

Managing extraction of colour, tannin and methoxypyrazines in Pinot noir grapes treated by leaf removal

Managing extraction of tannins and green aroma compounds attributed from methoxypyrazines in winemaking is crucial for producing high quality Pinot noir wine. This study1 investigated the impact of leaf removal on concentrations of anthocyanins, tannins, and methoxypyrazines in Pinot noir grapes and resultant wines.

Read More