WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Mining terroir influence on bioactive polyphenols from grape stems: A correlation-network-driven approach to spatialize metabolomics data

Mining terroir influence on bioactive polyphenols from grape stems: A correlation-network-driven approach to spatialize metabolomics data

Abstract

In viticulture, the concept of terroir is often used to enlighten the environmental-based typicity of grapevines grown in a local area however its scientific basis remains under debate. Grape polyphenols as key player of the plant defense system enables adaptation to environmental changes and so far, form a unique metabolic component to investigate the terroir influence. Using correlation-based networks and spatial metabolomics we investigated how continuous changes of soil properties may impact the polyphenol composition within a single grape clone. In a vineyard parcel covering four different geological layers and planted with a unique clone of Cabernet Franc, the soil texture was analyzed at 30 georeferenced points with a spatial coverage sampling strategy. Grape stems were harvested at corresponding positions over 3 consecutive years followed by UPLC-DAD-MS-based metabolomic analysis targeted on 43 metabolites including flavonoids, phenolic acids, procyanidins and stilbenoids. Principal component analyses on intra-vintage data presented good reproducibility. A correlation-driven approach was used to select co-varying metabolites before using Geographic Information System (GIS). As results, flavonoids and stilbenoid DP4 were spatialized according to soil granulometry, with stilbenoid DP4 over-accumulating in loamy-silty soils and flavonoids in sandy soils. The present study highlights soil-based terroir influence on polyphenols in a continuous space. Spatial metabolomics driven by correlation-based networks represents a powerful approach to spatialize field-omics data and may serve as new field-phenotyping tool in precision agriculture.

DOI:

Publication date: June 9, 2022

Issue: WAC 2022

Type: Article

Authors

Arnaud Lanoue, Kévin Billet, , Sébastien Salvador-Blanes, Thomas Dugé de Bernonville, Guillaume Delanoue, Florent Hinschberger, Audrey Oudin, Vincent Courdavault, Olivier Pichon, Sébastien Besseau, Samuel Leturcq, Nathalie Giglioli-Guivarc’h, Arnaud Lanoue

Presenting author

Arnaud Lanoue – EA 2106 Biomolécules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours

Institut Français de la Vigne et du Vin, Tours | GéoHydrosystèmes Continentaux (GéHCO), EA 6293, Université de Tours | Laboratoire CITERES, Equipe Laboratoire Archéologie et Territoires (LAT), UMR 7324 CNRS, Université de Tours| EA 2106 Biomolécules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours

Contact the author

Keywords

Terroir – metabolomics – grape polyphenols – Geographic Information System – correlation network

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Aim: This study aimed to evaluate whether irrigation of Touriga Nacional in Douro Demarcated Region (DDR) can partly mitigate the negative impacts of ongoing climate change on grapevine yield and quality and its impact on plant metabolism.

A new approach for sensory characterization of grape. Relationship with chemical composition

Characterize taste and mouthfeel properties of grapes elicited by the phenolic fraction (PF) of grape berries and establish relationships with chemical variables. METHODS: As many as 31 diverse grape lots of Tempranillo Tinto and Garnacha Tinta from three different regions were harvested. Grapes were destemmed and macerated in 15% of ethanol for one week and extracts were submitted to solid phase extraction. The recovered polyphenolic fraction was reconstituted in wine model and characterized by a panel of 21 wine experts employing a list of 23 taste and mouthfeel-related attributes following a rate-k-attributes methodology. RESULTS: Six significant attributes among the 31 samples differed based on ANOVA results: “dry”, “coarse”, “bitter”, “dry on tongue”, “sticky” and “watery”. PCA with VARIMAX algorithm was calculated.

EVALUATION OF INDIGENOUS SACCHAROMYCES CEREVISIAE ISOLATES FOR THEIR POTENTIAL USE AS FERMENTATION STARTERS IN ASSYRTIKO WINE

Assyrtiko is a rare ancient grape variety that constitutes one of the most popular in Greece. The objective of the current research was to evaluate indigenous Saccharomyces cerevisiae isolates as fermentation starters and also test the possible strain impact on volatile profile of Assyrtiko wine. 163 S. cerevisiae isolates, which were previously selected from spontaneous alcoholic fermentation, were identified at strain level by interdelta-PCR genomic fingerprinting. Yeasts strains were examined for their fermentative capacity in laboratory scale fermentation on pasteurized Assyrtiko grape must.

New use of natural silk fiber as a fining agent in wines

Undesirable compounds in wine, like OTA, biogenic amines, and pesticide residues, can negatively affect its quality and pose health risks to consumers. In addition, an excess of tannins can lead to an unpleasant rise in astringency and bitterness, which makes tannins another target of reduction.

Aroma profile evaluation in whole grape juices

Table grapes (Vitis labrusca and hybrids) are widely cultivated in Brazil [1] due to the climate, their resistance to disease and the way they are consumed and commercialized, either in-natura or for processing, producing whole juices, jams and table wines.