WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 2 - WAC - Posters 9 Acetaldehyde-induced condensation products in red wines affect the precipitation of salivary proteins. Will this impact astringency?

Acetaldehyde-induced condensation products in red wines affect the precipitation of salivary proteins. Will this impact astringency?

Abstract

Acetaldehyde is a common component of wine. It is already formed during the fermentation being an intermediate in the production of ethanol. Moreover, it can derive from the oxidation of ethanol during the wine production and aging. In wine, concentrations of acetaldehyde range from 30 to 130 mg/L. Acetaldehyde in wine can react with many compounds such as SO2, amino acids and polyphenols. The reaction between acetaldehyde and wine polyphenols takes place through a nucleophilic attack of polyphenols on the protonated form of the aldehyde,  affording methyl methine-linked dimers of two different units of polyphenolic structures, among others. The numerous and complex reactions trigged by acetaldehyde markedly influence the evolution of red wines during aging. Although numerous studies aimed to determine the chemical nature of reaction products in model solution and real wines, data concerning a possible change in reactivity of red wines towards salivary proteins due to acetaldehyde reactions are not known. This piece of information can be of great relevance as the interaction of wine with saliva and the precipitation of salivary proteins is a major phenomenon responsible for wine astringency. 

In the present work, to investigate the changes in the precipitation of salivary proteins after interaction with red wine, the effects of increasing concentrations of acetaldehyde (0-190 mg/L) in two wines with different polyphenolic composition (Aglianico and Tintilia) were studied over a 90-day period.

The impact of acetaldehyde reactions on the reactivity towards salivary proteins was determined by SDS-PAGE analysis of proteins before and after the reaction and Saliva Precipitation Index (SPI) was measured. 

For both wines a significant precipitation of colored matter was observed as a function of acetaldehyde concentration.  In all wines, a decrease of SPI due to acetaldehyde addition was detected. However, a different trend was observed in the two wines. In particular, Aglianico showed a greater decrease. The SPI of either Aglianico or Tintilia significantly changed over time along with polymeric pigments content as suggested by HPLC and MS analyses.

Overall, the results showed that the reactions in which acetaldehyde is involved exert important effects in the interactions between polyphenolic compounds and salivary proteins.

Therefore, the management of the acetaldehyde is to be properly addressed throughout all the stages of the winemaking process.

DOI:

Publication date: June 13, 2022

Issue: WAC 2022

Type: Article

Authors

Francesca Coppola, Martino Forino, Alessandra Rinaldi, Luigi Picariello, Massimo Iorizzo, Luigi Moio, Angelita Gambuti

Presenting author

Francesca Coppola – Department of Agricultural Sciences, Section of Vine and Wine Sciences, University of Naples ‘Federico II’, Viale Italia, 83100 Avellino, Italy

Department of Agricultural Sciences, Section of Vine and Wine Sciences, University of Naples ‘Federico II’, Viale Italia, 83100 Avellino, Italy | Department of Agricultural, Environmental and Food Sciences (DiAAA), University of Molise, Campobasso, Italy, University of Naples ‘Federico II’, Viale Italia, 83100 Avellino, Italy

Contact the author

Keywords

Acetaldehyde, Precipitation of Salivary Proteins, Red wine, Phenolic compounds

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Sustainable fertilisation of the vineyard in Galicia (Spain)

Excessive fertilization of the vineyard leads to low quality grapes, increased costs and a negative impact on the environment. In order to establish an integrated management system aimed at a sustainable fertilization of the vineyards, nutritional reference levels were established. For this purpose, 30 representative vineyards of the Albariño variety were studied, in which soil and petiole analyses were carried out for two years and grape yield and quality at harvest were measured. In both years of study, soil pH, calcium, sodium and cation exchange capacity were positively correlated with calcium content and negatively correlated with manganese in grapes. Irrigated vineyards had higher levels of aluminium in soil and lower levels of calcium in petiole. Climatic conditions were very different in the years of the study. The year 2019 was colder than usual, in 2020 there was a marked water stress with high summer temperatures. This resulted in medium-high acidity in grapes in 2019 and low acidity in 2020, with sugar levels being similar both years. A very marked decrease in must amino nitrogen was observed in 2020, with ammonia nitrogen remaining stable. The correlation of acidity and sugar values in grapes with soil and petiole analysis data made it possible to establish reference levels for the nutritional diagnosis of the Albariño variety in this region. Based on these results, an easy-to-use TIC application is currently being created for grapegrowers, aimed at improving the sustainability of the vineyard through reasoned fertilization. This study has now been extended to other Galician vine varieties.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Phenolic composition of Tempranillo Blanco grapes changes after foliar application of urea

Our research aimed to determine the effect and efficiency of foliar application of urea on the phenolic composition of Tempranillo Blanco grapes. The field experiment was carried out in 2019 and 2020 seasons and the plot was located in D.O.Ca Rioja (North of Spain). The vineyard was Vitis vinifera L. Tempranillo Blanco and grafted on Richter-110 rootstock. The treatments were control (C), whose plants were sprayed with water and three doses of urea: plants were sprayed with urea 3 kg N/ha (U3), 6 kg N/ha (U6) and 9 kg N/ha (U9). The applications were performed in two phenological stages, pre-veraison (Pre) and veraison (Ver). Also, each of the treatments was repeated one week later. Control and treatments were performed in triplicate and arranged in a randomised block design. Grapes were harvested at optimum ripening stage. High-performance liquid chromatography was used to analyse the phenolic composition of the grapes. Finally, the results obtained from the analytical determinations – flavonols, flavanols and non-flavonoid (hydroxybenzoic acids, hydroxycinnamic acids and stilbenes) – were studied statistically by analysis of variance. The results showed that, in 2019, U6-Pre and U9-Pre treatments increased the hydroxybenzoic acid content in grapes, and also all foliar treatments applied at Pre enhanced the stilbene concentration. Moreover, U3-Ver was the only treatment that rose flavonol and stilbene contents in the Tempranillo Blanco grapes. In 2020, all treatments applied at Pre enhanced the flavonol concentration in grapes. Furthermore, U3-Pre and U9-Pre treatments increased stilbene content in grapes. Nevertheless, the hydroxybenzoic acid content was improved by U6-Ver and U9-Ver and besides, hydroxycinnamic acid concentration in grapes was increased by all treatments applied at Ver. In conclusion, the lower and highest dose of urea (U3 and U9), applied at pre-veraison, were the best treatments to improve the Tempranillo Blanco grape phenolic composition.

Comparison of imputation methods in long and varied phenological series. Application to the Conegliano dataset, including observations from 1964 over 400 grape varieties

A large varietal collection including over 1700 varieties was maintained in Conegliano, ITA, since the 1950s. Phenological data on a subset of 400 grape varieties including wine grapes, table grapes, and raisins were acquired at bud break, flowering, veraison, and ripening since 1964. Despite the efforts in maintaining and acquiring data over such an extensive collection, the data set has varying degrees of missing cases depending on the variety and the year. This is ubiquitous in phenology datasets with significant size and length. In this work, we evaluated four state-of-the-art methods to estimate missing values in this phenological series: k-Nearest Neighbour (kNN), Multivariate Imputation by Chained Equations (mice), MissForest, and Bidirectional Recurrent Imputation for Time Series (BRITS). For each phenological stage, we evaluated the performance of the methods in two ways. 1) On the full dataset, we randomly hold-out 10% of the true values for use as a test set and repeated the process 1000 times (Monte Carlo cross-validation). 2) On a reduced and almost complete subset of varieties, we varied the percentage of missing values from 10% to 70% by random deletion. In all cases, we evaluated the performance on the original values using normalized root mean squared error. For the full dataset we also obtained performance statistics by variety and by year. MissForest provided average errors of 17% (3 days) at budbreak, 14% (4 days) at flowering, 14.5% (7 days) at veraison, and 17% (3 days) at maturity. We completed the imputations of the Conegliano dataset, one of the world’s most extensive and varied phenological time series and a steppingstone for future climate change studies in grapes. The dataset is now ready for further analysis, and a rigorous evaluation of imputation errors is included.