Non Saccharomyces wine yeasts: emerging trends and challenges in winemaking

The studies developed by INRA and UV, in Angers, concern wine-growing areas and their optimized management, both from an agro-viticultural and oenological point of view. Previous work (Morlat, 1989) made it possible to give a scientific dimension to the concept of viticultural terroir and demonstrated the considerable influence of this production factor on the quality and typicity of wines (Asselin et al, 1992 ) . A methodology for the integrated characterization of terroirs, based on the “Basic Terroir Natural Unit” (considered as the smallest spatial unit of territory usable in practice, and in which the response of the vine is homogeneous), has been development (Riou et al , 1995).

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.

UV-Visible spectroscopy in conjunction with chemometrics, was successfully applied to objectively differentiate sparkling wine press juice fractions of Pinot noir. Two measurements methods were applied: reflectance using a fibre optic probe in-line and transmission using a benchtop spectrophotometer.

Da dove scaturisce tutto l’interesse attuale per il terroir? Si provi, per dare risposta a questo quesito, ad immaginare il vino avulso dalla sua dimensione territoriale. Cosa si otterrebbe? Un vino bianco, un vino rosso o quant’altro, ma comunque un prodotto privo di conno­tazione geografica, di premesse storiche, di radici tradizionali, di potere evocativo, di iden­tità e di personalità.

Viticulture needs for spatial and temporal information are increasing to improve vineyard management, especially concerning water efficiency. Remote sensing, particularly from satellites, can be a powerful tool to assess vineyard characteristics such as vigor or water status in space-time. In this study, we use Landsat 8, an American Earth observation satellite with six bands from the visible (VIS) to the Short-Wave Infrared (SWIR) domains with 30m spatial resolution and two thermal bands with 100m spatial resolution.