WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Oral 9 What happens with the glutathione during winemaking and the storage of the wine?

What happens with the glutathione during winemaking and the storage of the wine?

Abstract

We tried to give a part of the answer to this question by monitoring glutathione during winemaking and storage. The novelty of our approach is to quantify simultaneously the three known forms of glutathione: free glutathione (GSH), oxidized form (GSSG) and glutathione-S-sulfonate (GSSO3H). This last molecule was reported first in wine by Arapatsis et al. (2016), who described the reaction between SO2 and GSSG resulting GSH and GSSO3H. Theoretically, GSH can further react with SO2, but this reaction is slow. This results obtained in model wine was now proved in grape juice, by measuring the kinetic of the reaction after the addition of SO2. For this purpose a LC-MS/MS analysis method was developed, which allows for the first time the quantification of GSSO3H beside of GSH and GSSG in the wine. 

The analyses of samples taken in the different moment of winemaking shows that in the must only GSH and GSSG are present. GSSO3H appears after the addition of SO2 at the end of the alcoholic fermentation. It appears also to be the dominant form of glutathione in SO2 containing wines after 3 months storage. The analysis of a hundred of wines showed a correlation between the relative concentration of GSSO3H and the total SO2 level of the wine. Temperature also effects the reaction rate. Grape variety does not seem to influence the formation of GSSO3H. 

We could conclude that SO2 contribute to release active GSH from the GSSG and so extend the protection potential against oxidation during the first months of storage. It remains the questions: Does GSSO3H have any antioxidant activity? Further investigation would be needed to address this question. However, in the meantime, its quantification is important in the wine to avoid underestimating the glutathione content.

DOI:

Publication date: June 14, 2022

Issue: WAC 2022

Type: Article

Authors

Ágnes Dienes-Nagy, Frédéric Vuichard, Sandrine Belcher, Marie Blackford, Johannes Rösti, Fabrice Lorenzini

Presenting author

Ágnes Dienes-Nagy – Agroscope, 1260 Nyon, Switzerland

Agroscope, 1260 Nyon, Switzerland | Agroscope, 1260 Nyon, Switzerland | Agroscope, 1260 Nyon, Switzerland| Agroscope, 1260 Nyon, Switzerland | Agroscope, 1260 Nyon, Switzerland 

Contact the author

Keywords

glutathione, wine, glutathione-S-sulfonate, LC-MS/MS

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

A survey on the rotundone content of 18 grape varieties sourced from a germplasm 

Rotundone, the pepper aroma compound, has been detected in wines made from a large number of grape varieties. However, given the fact that analyzed wines were sourced from different winegrowing regions and seasons, made using different winemaking techniques and at different scales, it remains difficult to assess the real variety potential to produce rotundone.

Application de l’Analyse du Cycle de Vie (ACV) à un domaine viticole

Since 1980, Château de l’Éclair has belonged to SICAREX Beaujolais and has been involved in experimentation for the Beaujolais vineyards. However, it is a commercial estate with profitability and quality constraints, which means that it has to meet the growing environmental expectations of consumers. Given the number of practices claimed to be environment-friendly, it is sometimes difficult to prioritize actions.

Tropical fruit aroma in white wines: the role of fermentation esters and volatile thiols

Volatile thiols are impact aroma compounds, well-known in the literature for imparting tropical fruit aromas such as passion fruit, guava, grapefruit, and citrus in white wines [1]. More recent evidence suggests that tropical fruit aromas are also caused by other aroma compounds besides thiols, such as fermentation esters, or the interaction between these volatile families. Therefore, the objective of this study was to investigate the effects of combining esters and/or thiols to determine their impact on the fruitiness aroma perception of white wines. Pinot gris wine was produced at the OSU research winery and was dearomatized using Lichrolut® EN. Combinations of fermentation volatile compounds were added to the wine, forming the aroma base. Treatment wines were composed of additions of different concentrations and combinations of thiols and/or esters. Samples were subjected to sensory analysis where forty-six white wine consumers evaluated the orthonasal aroma of the wines and participated in Check-All-That-Apply (CATA).

Stable or dynamic? How phenotypic plasticity could be key to select for grapevine adaptation?

Climate change will require the adaptation of agricultural systems and among the different means of adaptation, changing plant material is a promising strategy. In viticulture, different levels of diversity are currently exploited: clonal and varietal diversity for rootstocks and scions. A huge quantity of research aims to evaluate different genotypes in different environmental conditions to identify which ones are the best adapted and the most tolerant to future environmental conditions.

Eléments importants d’une méthodologie de caractérisation des facteurs naturels du terroir, en relation avec la réponse de la vigne à travers le vin

The French viticultural appellation areas are the result of an empirical, historical and evolutionary selection which, generally, has consecrated a match between natural factors, grape varieties and viti-vinicultural practices. The notion of terroir is the main basis of the Appellation d’Origine Contrôlée in viticulture. It is based on the one hand on privileged natural factors and on the other hand on the know-how of the winegrowers; the whole allowing the production of a wine endowed with an authenticity and a sensory typicity. Wine-growing practices evolve according to progress in viticulture and oenology, while the natural factors of the terroir are much more stable, with the exception of the vintage. They therefore represent a fundamental pillar of the identity of an appellation vineyard.