IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Isolated Antarctic soil yeasts with fermentative capacity with potential use in the wine industry

Isolated Antarctic soil yeasts with fermentative capacity with potential use in the wine industry

Abstract

The wine industry is currently on the search for new aromas and less browning in their products. In the improvement process of wine, lower fermentation temperatures have been considered, however, the yeasts in the market cannot tolerate such temperatures. Therefore, an interesting place to find new yeasts with criotolerance is the Antarctic Continent.Our methodology to accomplish our objectives started with the isolation of yeast from soil using YM agar (yeast extract, malt extract, peptone, 1% glucose, and agar) with antibiotics. Then the isolates were submitted to a fermentation assay using a colorimetric indicator of pH variation. The samples positive for fermentative activity were then analyzed to determine tolerance to glucose:fructose 1:1, with the following concentration; 5,10,15,20, and 25%. The isolates were analyzed by their capacity to grow at different alcohol concentrations (3,6,9%).Then fingerprinting analysis was performed to select unique yeasts. Then ITS region was amplified and sequenced to identify the isolates.We were able to isolate 125 yeasts. Of which 25 had fermentative activity at 10ºC. These yeasts were used to analyze glucose tolerance and alcohol tolerance. All samples grew at 20% sugar content, and all samples grew at 6% alcohol content. Some of the isolates were capable of growing at the most extreme conditions, with 25% sugar content and 9% ethanol.After a fingerprinting assay, we reduced the candidates to 9 isolates. Then we determined the optimal growth temperature where we observed that our isolates have a longer latency period regarding S. cerevisiae, the Antarctic isolates grew better at 10 and 15ºC. Later on, we extracted DNA, performed a PCR for the ITS region, and sequenced the ITS regions to identify the isolates from Antarctica. Finally, the ITS sequenced regions were used to create a phylogenetic tree.The fermentative yeasts with high alcohol tolerance and fermentation at high concentrations of sugar will be used for micro-fermentation of synthetic must to determine their potential use in the production of Chilean wine

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Navarro Laura1, Gil Mariona2, Gutiérrez Ana1,3, Calisto Nancy1,4, Úbeda Cristina1,5 and Corsini Gino1

1Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma, Santiago, Chile
2Instituto de Ciencias Químicas Aplicadas, Facultad de Ingeniería, Universidad Autónoma, Santiago, Chile
3Facultad de Ciencias Agropecuarias y Forestales, Departamento de Producción Agropecuaria Universidad de La Frontera, Temuco. Chile
4Centro de Investigación y Monitoreo Ambiental Antártico (CIMAA), Departamento de Ingeniería Química, Universidad de Magallanes, Avenida Bulnes 01855, Punta Arenas, Chile
5​​Área de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, España.

Contact the author

Keywords

wine, wild yeast, fermentation at cold temperatures, browning

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

A comprehensive ecological study of grapevine sensitivity to temperature; how terroir will shift under climate change

Fossil fuel combustion continues to drive increases in atmospheric carbon dioxide, consequently elevating the global annual mean temperature and specifically increasing the growing season temperatures in many of the world’s most important wine growing regions (IPCC 2014; Jones et al 2005). Grapes are sensitive to changes in growing season temperatures, and past models have shown a direct link between warming temperatures and earlier harvest dates (Cook and Wolkovich 2016). Globally, there have been shifts of 1-2 weeks for wine growing regions (Wolkovich et al 2017 and references within). The phenological shifts resulting from growing season temperature increases are documented internationally, and models predicting phenology using temperature are becoming more precise (Parker et al 2011).

Territoire, terroir et marché du vin à la production

Work aimed at understanding the relationship between a terroir, in the agronomic sense, and the physico-chemical characteristics of grapes or wine are numerous today, as evidenced by the program of this symposium. But for an economist, the central question remains to know how the terroir can intervene in the construction of the economic value of wine and in the differentiation of its prices. Is the terroir effect recognized by the end consumer or is it only an internal adjustment variable in the production systems? Through which indicators can this terroir effect be managed by the various operators in the sector? In the end, isn’t it better to invoke a “territorial effect” that the actors can build, and of which the terroir would be one of the possible components?

Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Botrytis cinerea (Bc) is one of the main pathogens affecting the cultivated grapevine. A key role in grapevine tissue colonization is played by cell wall (CW) remodeling driven by CW Modifying Enzymes (CWMEs), expressed both by the host and the pathogen. Their action can impact CW integrity and trigger specific immune signaling, thus influencing Bc infection outcome. To further characterize the role of the CW in the grapevine response to Bc, two contrasting genotypes in their resistance to the fungus were artificially inoculated at full bloom. RNA-seq analysis and biochemical characterization of the CW and its modification in samples collected at 24 hours post-inoculation highlighted significant differences between genotypes.

Influence of the vineyard’s surrounding vegetation on the phenolic potential of Vitis vinifera L. cv Tempranillo grapes

Wine industry has to develop new strategies to reduce the negative impact of global climate change in wine quality while trying to mitigate its own contribution to this climate change. The term “ecosystem services”, whose use has been recently increasing, refers to the benefits that human beings can obtain from the interactions between the different living beings that coexist in an environment or system. The management of biodiversity in the vineyard has a positive impact on this crop. It has recently been reported that practices such as plant cover can reduce the occurrence of pests, increase pollination of the vine, improve plant performance1 and affect the phenolic content of grapes.2

Taking advantage of difficulties. Variable rate application based on canopy maps to achieve a sustainable crop

Aim: The aim of this work was to evaluate the use of Variable Rate Application technologies based on prescription maps in commercial vineyards with large intra-parcel variability to achieve a more sustainable distribution of Plant Protection Products (PPP)