IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Isolated Antarctic soil yeasts with fermentative capacity with potential use in the wine industry

Isolated Antarctic soil yeasts with fermentative capacity with potential use in the wine industry

Abstract

The wine industry is currently on the search for new aromas and less browning in their products. In the improvement process of wine, lower fermentation temperatures have been considered, however, the yeasts in the market cannot tolerate such temperatures. Therefore, an interesting place to find new yeasts with criotolerance is the Antarctic Continent.Our methodology to accomplish our objectives started with the isolation of yeast from soil using YM agar (yeast extract, malt extract, peptone, 1% glucose, and agar) with antibiotics. Then the isolates were submitted to a fermentation assay using a colorimetric indicator of pH variation. The samples positive for fermentative activity were then analyzed to determine tolerance to glucose:fructose 1:1, with the following concentration; 5,10,15,20, and 25%. The isolates were analyzed by their capacity to grow at different alcohol concentrations (3,6,9%).Then fingerprinting analysis was performed to select unique yeasts. Then ITS region was amplified and sequenced to identify the isolates.We were able to isolate 125 yeasts. Of which 25 had fermentative activity at 10ºC. These yeasts were used to analyze glucose tolerance and alcohol tolerance. All samples grew at 20% sugar content, and all samples grew at 6% alcohol content. Some of the isolates were capable of growing at the most extreme conditions, with 25% sugar content and 9% ethanol.After a fingerprinting assay, we reduced the candidates to 9 isolates. Then we determined the optimal growth temperature where we observed that our isolates have a longer latency period regarding S. cerevisiae, the Antarctic isolates grew better at 10 and 15ºC. Later on, we extracted DNA, performed a PCR for the ITS region, and sequenced the ITS regions to identify the isolates from Antarctica. Finally, the ITS sequenced regions were used to create a phylogenetic tree.The fermentative yeasts with high alcohol tolerance and fermentation at high concentrations of sugar will be used for micro-fermentation of synthetic must to determine their potential use in the production of Chilean wine

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Navarro Laura1, Gil Mariona2, Gutiérrez Ana1,3, Calisto Nancy1,4, Úbeda Cristina1,5 and Corsini Gino1

1Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma, Santiago, Chile
2Instituto de Ciencias Químicas Aplicadas, Facultad de Ingeniería, Universidad Autónoma, Santiago, Chile
3Facultad de Ciencias Agropecuarias y Forestales, Departamento de Producción Agropecuaria Universidad de La Frontera, Temuco. Chile
4Centro de Investigación y Monitoreo Ambiental Antártico (CIMAA), Departamento de Ingeniería Química, Universidad de Magallanes, Avenida Bulnes 01855, Punta Arenas, Chile
5​​Área de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, España.

Contact the author

Keywords

wine, wild yeast, fermentation at cold temperatures, browning

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

A geologic numeric mapping, with geophysic transects, of Marsannay’s vineyard (Burgundy, France)

Marsannay est l’appellation la plus septentrionale de la Côte de Nuits, à la limite Sud de Dijon. Elle regroupe trois villages, Chenôve, Marsannay-la-Côte et Couchey, et constitue l’appellation la plus vaste de la Côte de Nuits avec 315 hectares en A.O.C. Elle possède uniquement des niveaux d’appellations régionales et communales depuis 1987.

Influence of irrigation on water status, productivity, yield and must composition in Tempranillo grapevine under Duero Valley zone conditions

Cette étude a pour but d’évaluer la modification de l’état hydrique (potentiel hydrique foliaire), le comportement productif (matière sèche et rendement) et la expression qualitative (poids de baie, degrée Brix, pH, acidité totale, concentration polyphénolique) de la varieté Tempranillo dans la Vallée du Douro, à l’A.O. Cigales, comme conséquence de l’application d’une irrigation modérée. Pour développer l’essai on a appliqué les suivantes

Model-assisted analysis of the root traits underlying RSA genotypic diversity in Vitis: a promising approach for rootstock selection?

By dissecting the root system architecture (RSA) into its underpinning components (e.g. root emission, axial growth, radial growth, branching, root direction or tropism) and identifying the relationships between them, functional-structural 3D root models are promising tools for analyzing the diversity and complexity of root system phenotypes with Genotype × Environment interactions. The model parameters are assumed to be synthetic traits, less influenced by the environment, and consequently with less polygenic architectures than the integrative RSA traits they drive. Root models can serve as a basis for in silico development of root system ideotypes by highlighting the developmental processes and parameters that most likely influence RSA fitness.

Comparing different vineyard sampling densities and patterns for spatial interpolation of intrinsic water use efficiency

The need to rationalize agricultural inputs has recently increased interest in assessing vineyard variability in order to implement variable rate input applications, so-called ‘precision viticulture’. In many viticultural areas globally, precision viticulture is already widely used such as for selective harvesting and variable rate application (VRA) of inputs such as irrigation and/or fertilizer. Robust VRA relies on having a geostatistically accurate map (of one or more vineyard attributes) requiring high sampling densities, which can be cost- and time-prohibitive to obtain. Previous work on spatial interpolation using kriging have upscaled ground-based measurements, but such upscaling strategies are applicable only when vineyard conditions are spatially continuous and satisfies the assumption of second-order stationary processes. Alternatively, mixed models that combine kriging and auxiliary information, such as the regression kriging (RK) method, are more instructive for spatial predictions. In order to improve prediction accuracies, it is therefore necessary to incorporate additional information to achieve accurate spatial patterns with low error.

Behavior of disease resistant grapevine varieties to downy mildew (Plasmopara viticola) infections in the Castelli Romani area (Central Italy)

At CREA – Centro di Ricerca di Viticoltura ed Enologia, based in Velletri (RM), an experimental vineyard including 10 downy mildew resistent/tolerant grape varieties and two susceptible varieties was set up with the principal goal to evaluate the behavoir of these varieties in term of resistance to downy mildew (Plasmopara viticola). This evaluation, together to oenological studies, are necessary to register them also in Regional Register (in Lazio region). Monitoring of behavior towards Plasmopara viticulture of resistant vines were done in 2020 and 2021 at different times (phenological stages) and until harvesting, according to an international standard code BBCH a centesimal phenological scale, based on coding system.