IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 A new chemiluminescence method related to molecules derived from Botrytis cinerea for characterization of Aszu wines from Tokaj, from Hungary

A new chemiluminescence method related to molecules derived from Botrytis cinerea for characterization of Aszu wines from Tokaj, from Hungary

Abstract

For the chemical characterization of Aszu wines from Tokaj region our aim is to develop a biochemical method which is related to Botrytis cinerea. As in these wines there are continuously present small amounts of Cytochrome C enzyme derived from apoptotic cells infected with Botrytis (1), and H2O2 produced during the oxidation of alcohols by Botrytis (2), and their oxidative interaction can produce reactive “ferril-peroxide complexes  inducing chemiluminescence (photon emission) (3), the  principle of method is as follows: the capability of various Aszu wines is measured by luminometer how they can stimulate the production of chemiluminescence of basal biochemical reaction (Cytochrome C + H2O2) compared to a standard solution of „artificial furmint”. The size of stimulation is expressed numerically by „Index of Stimulation” (I.S.). Based on a great number of measurements evaluated by statistical methods we created three categories for the quality of Aszu wines from the aspect of Botrytis effects valid for given laboratory condition. „Tokaj Aszu of poor quality”: I. S. < 2,79„Tokaj Aszu of outstanding quality”: I.S. = 2,80-3,54„Tokaj Aszu of excellent quality”: S.I. > 3,55 These categories correlate well with other factors of Aszu quality (aroma, flavour, smell) but do not cover them totally.  The I.S. values of Aszu wines show a significantly positive correlation with the concentrations of gluconic acid (p

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Sándor Sipka1, NagyAndrea1, Csősz Éva2 and Baráth Sándor1

1Division of Clinical Immunology University of Debrecen, Móricz Zs. Str. 22. 4032 Debrecen, Hungary
2Department of Biochemistry and Molecular Biology University of Debrecen, Hungary

Contact the author

Keywords

chemiluminescence assay, Botrytis cinerea, Aszu of Tokaj, Cytochrome C, hydrogen peroxide

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

FOURIER TRANSFORM INFRARED SPECTROSCOPY IN MONITORING THE WINE PRODUCTION

The complexity of the wine matrix makes the monitoring of the winemaking process crucial. Fourier Transform Infrared Spectroscopy (FTIR) along with chemometrics is considered an effective analytical tool combining good accuracy, robustness, high sample throughput, and “green character”. Portable and non-portable FTIR devices are already used by the wine industry for routine analysis. However, the analytical calibrations need to be enriched, and some others are still waiting to be thoroughly developed.

Read More

Ultra high pressure liquid chromatography for stilbenes separation and their determination in Burgundy red wines

In this study for the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin, trans-piceatannol, (+)-trans-s-viniferin, pallidol and hopeaphenol) isolated and purified from Vitis vinifera, were simultaneously separated and analysed within 5 mn by ultra high pressure liquid chromatography coupled with photodiode array detection.

Read More

Testing the pathogen e-learning and field training course on grapevine virus knowledge and management

One of the reasons of the spread of grapevine virus diseases in
vineyards around the world is the lack of knowledge by the main actors of the wine sector. To face this problem, five partners worked together to develop the PAThOGEN project, a training program aimed to improve grapevine virus knowledge and management. The partnership gathers one French technical center (IFV), one Spanish university (USC), one Italian applied research center (CREA), one Spanish foundation
specialized in training and technology transfer (FEUGA) and one Italian SME specialized in the development of informatics tools and in knowledge transfer (HORTA).The objectives of PAThOGEN are: (i) to develop and
maintain a high-quality work-based Vocational and Education Training program, (ii) to improve the skills of professionals of the wine sector.

Read More

Untangle berry shrivel environmental risk factors and quantify symptoms with AI – GeomAbs meets BAISIQ

Berry Shrivel (BS, Traubenwelke) is a sugar accumulation disorder of grapevine of unknown causes, having a great negative impact on grape quality and incalculable risks for yield losses, and for which no reliable curative practices are available.

Read More

Impact of chitosan treatment on the physico-chemical features of a sangiovese red wine

Chitosan is gaining interest in red winemaking thanks to its ability to inhibit the development of Brettanomyces spp. yeast, or other undesired wine microbial threats. However, little is known about potential side-effects of its addition on the physico-chemical parameters of red wines.

Read More