IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Predictive Breeding for Wine Quality: From Sensory Traits to Grapevine Genome

Predictive Breeding for Wine Quality: From Sensory Traits to Grapevine Genome

Abstract

New pathogen resistant varieties allow an efficient and greatly reduced use of fungicides. These new varieties promise, therefore, an enormous potential to reach the European Green Deal aim of a 50% reduction of pesticides in EU agriculture by 2030. The selection process, and particularly quality evaluation of the wines produced, are a bottleneck slowing down the breeding of new pathogen resistant grapevine varieties. Our major aim is therefore the development of predictive models for wine quality traits. Their implementation in the selection process would considerably increase the efficacy of grapevine breeding.The centrepiece of our study is a segregating white wine F1-population of ‘Calardis Musqué’ and ‘Villard Blanc’ consisting of 150 genotypes with 13 plants per genotype at two locations. A ‘Genotyping by Sequencing’ approach with a novel bioinformatics pipeline delivered a high-density genetic map of the breeding population. Experimental winemaking in a 4-liter scale (micro-vinification) provided authentic wines for comprehensive sensory evaluation and chemical analysis of major and minor metabolites including aroma compounds such as monoterpenoids. Moreover, five annual repetitions at two locations allow robust modelling and an estimation of environmental impact on the phenotypic data. Genetic, metabolic, and sensory data for multiple vintages combine into a comprehensive data base for predictive modelling. The descriptive and quality score card was adapted to the large number of wine samples and the unusual broad range of wine qualities resulting from an unselected set of grapevine genotypes. Based on evaluation of all 150 genotypes we differentiated a set of best and worst wines reproducibly over years. Environmental-related differences among vintages were still present. Intensity of the descriptive attribute “floral” played a crucial role for total quality within this population and correlates with linalool and cis-rose oxide concentration of the wines in all vintages measured by SIDA-SPE-GC-MS. In addition, total concentrations of linalool enabled the discovery of several genomic regions (quantitative trait loci, QTLs) that collocate with putative genes associated with terpene biosynthesis. Multi seasonal data allowed refinement and validation of models predicting these wine quality traits. Further exploitation of the large data set will provide more insights into genomic regions related to other wine quality traits and will allow an early selection of genotypes of promising genetic quality potential or sorting out of poor candidates during grape vine breeding.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Siebert, Annemarie1, Vestner Jochen1, Röckel Franco2, Schwander Florian2, Frenzke Lena3, Wenke Torsten4, Wanke Stefan3, Töpfer Reinhard2 and Fischer Ulrich1

1Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Institute for Viticulture and Oenology
2Julius Kühn-Institute (JKI), Institute for Grapevine Breeding, Geilweilerhof
4ASGEN GmbH & Co. KG

Contact the author

Keywords

Wine quality, metabolic quality potential, monoterpenes, genetic quality potential, quantitative trait loci

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Grape berry size is a key factor in determining New Zealand Pinot noir wine composition

Making high quality but affordable Pinot noir (PN) wine is challenging in most terroirs and New Zealand’s (NZ) situation is no exception. To increase the probability of making highly typical PN wines producers choose to grow grapes in cool climates on lower fertility soils while adopting labour intensive practices. Stringent yield targets and higher input costs necessarily mean that PN wine cost is high, and profitability lower, in line-priced varietal wine ranges. To understand the reasons why higher yielding vines are perceived to produce wines of lower quality we have undertaken an extensive study of PN in NZ. Since 2018, we established a network of twelve trial sites in three NZ regions to find individual vines that produced acceptable commercial yields (above 2.5kg per vine) and wines of composition comparable to “Icon” labels. Approximately 20% of 660 grape lots (N = 135) were selected from within a narrow juice Total Soluble Solids (TSS) range and made into single vine wines under controlled conditions. Principal Component Analysis of the vine, berry, juice and wine parameters from three vintages found grape berry mass to be most effective clustering variable. As berry mass category decreased there was a systematic increase in the probability of higher berry red colour and total phenolics with a parallel increase in wine phenolics, changed aroma fraction and decreased juice amino acids. The influence of berry size on wine composition would appear stronger than the individual effects of vintage, region, vineyard or vine yield. Our observations support the hypothesis that it is possible to produce PN wines that fall within an “Icon” benchmark composition range at yields above 2.5kg per vine provided that the Leaf Area:Fruit Weight ratio is above 12cm2 per g, mean berry mass is below 1.2g and juice TSS is above 22°Brix.

Soil quality in Beaujolais vineyard. Importance of pedology and cultural practices

A pedological study was carried out from 2009 to 2017 in Beaujolais vineyard, to improve physical and chemical knowledge of soils. It was completed in 2016 and 2017 by the current study, dealing with microbial aspects, in order to build a reference frame for improved advice in soil management. Microbial biomass was measured on representative plots of the six most common soil types identified in Beaujolais and, for each soil type, on plots with different levels of the main impacting parameters: total organic carbon, pH, cation exchange capacity, extractable copper. A total of 59 soil samples were collected. Confirming the results of various trials carried out in Beaujolais over the past 20 years, the results of the present study showed that the soils were still alive, but exhibited a large variability of biological parameters, which appeared dependant on both pedological and anthropic factors. Therefore, a good interpretation of biological parameters and advice for vine growers must rely on a pedologically-based referential with differentiated main driving factors. For example, the control of pH is of primary importance in granitic soils and in no way organic matter addition can improve soil quality if pH is too low. Conversely, in calcareous soils, biological parameters are more directly affected by direct or indirect (cover crops for example) inputs of organic matter. The use of biological parameters, such as microbial biomass, is of great potential value to improve advice on agro-viticultural practices (soil management, fertilization, liming, etc.), basis of a sustainable wine production on fragile soils.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)