IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Abstract

Grapes infected with grey mould due Botrytis cinerea are widespread in vineyards during certain growing conditions.  Excessive infection levels may lead to decreased yields and the formation of off flavours in wine made from infected grapes. To assist in timely vineyard management that minimises yield and quality losses, decision support tools that correlate early detection of Botrytis infection and quantification of potential off flavour development is desirable.In this study, laboratory infection of whole bunches/ single berries with Botrytis cinerea to create a range of grey mould contamination in Shiraz, Cabernet Sauvignon, Chardonnay and Semillon were undertaken. After SPME GC-MS detection of grape homogenate, 8 out of 22 volatile compounds, including 3-octanol, 3-octanone, 1,5-dimethylnapthalene and 1,5-dimethyltetralin, were identified from VIP score and selectivity ratio, and excellent predictive model of Botrytis cinerea infection levels (determined by ergosterol measurement, antigen capture and qPCR) were developed using PLS and PLS2. These compounds, with high predictive accuracy, could be considered as potential biomarkers for rapid MS techniques in early stage.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Jiang Liang1, Qiu Y.1,2, Dumlao M.1,2,3, Donald W. A.4, Steel C. C.1,2 and Schmidtke L. M.1,2,3

1School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science, Charles Sturt University
2Gulbali Institute (Agriculture Water Environment), Charles Sturt University
3The Australian Research Council Training Centre for Innovative Wine Production, University of Adelaide

4School of Chemistry, Faculty of Science, University of New South Wales

Contact the author

Keywords

Grape disease, Grey mould, Botrytis cinerea, SPME GC-MS, Volatile organic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Understanding vine response to Mediterranean summer stress for the development of adaptation strategies – in the kaolin case

In this video recording of the IVES science meeting 2023, Sara Bernardo (CITAB, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal) speaks about understanding vine response to Mediterranean summer stress for the development of adaptation strategies – in the kaolin case. This presentation is based on an original article accessible for free on OENO One.

Terroir influence on growth, grapes and grenache wines in the AOC priorat, northeast Spain

The Mediterranean climate of The Priorat AOC, situated behind the coastal mountain range of Tarragona, tends towards continentality with very little precipitation during the vegetation cycle. The soil is poor, dry and rocky, largely composed of slate schist, known as “llicorella”. Vines primarily grow on steep slopes and terraces.

Exploring the effect of oxygen exposure during malolactic fermentation on red wine color

this research investigates the impact of early oxygen exposure, also during malolactic fermentation (MLF), on pigments and color of a red wine from Sangiovese grapes

Acetaldehyde-induced condensation products in red wines affect the precipitation of salivary proteins. Will this impact astringency?

Acetaldehyde is a common component of wine. It is already formed during the fermentation being an intermediate in the production of ethanol. Moreover, it can derive from the oxidation of ethanol during the wine production and aging. In wine, concentrations of acetaldehyde range from 30 to 130 mg/L. Acetaldehyde in wine can react with many compounds such as SO2, amino acids and

Genotypic variability in root architectural traits and putative implications for water uptake in grafted grapevine

Root system architecture (RSA) is important for soil exploration and edaphic resources acquisition by the plant, and thus contributes largely to its productivity and adaptation to environmental stresses, particularly soil water deficit. In grafted grapevine, while the degree of drought tolerance induced by the rootstock has been well documented in the vineyard, information about the underlying physiological processes, particularly at the root level, is scarce, due to the inherent difficulties in observing large root systems in situ. The objectives of this study were to determine genetic differences in the root architectural traits and their relationships to water uptake in two Vitis rootstocks genotypes (RGM, 140Ru) differing in their adaptation to drought. Young rootstocks grafted upon the Riesling variety were transplanted into cylindrical tubes and in 2D rhizotrons under two conditions, well watered and moderate water stress. Root traits were analyzed by digital imaging and the amount of transpired water was measured gravimetrically twice a week. Root phenotyping after 30 days reveal substantial variation in RSA traits between genotypes despite similar total root mass; the drought-tolerant 140Ru showed higher root length density in the deep layer, while the drought-sensitive RGM was characterised by shallow-angled root system development with more basal roots and a larger proportion of fine roots in the upper half of the tube. Water deficit affected canopy size and shoot mass to a greater extent than root development and architectural-related traits for both 140Ru and RGM, suggesting vertical distribution of roots was controlled by genotype rather than plasticity to soil water regime. The deeper root system of 140Ru as compared to RGM correlated with greater daily water uptake and sustained stomata opening under water-limited conditions but had little effect on above-ground growth. Our results highlight that grapevine rootstocks have constitutively distinct RSA phenotypes and that, in the context of climate change, those that develop an extensive root network at depth may provide a desirable advantage to the plant in coping with reduced water resources.