IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Abstract

Grapes infected with grey mould due Botrytis cinerea are widespread in vineyards during certain growing conditions.  Excessive infection levels may lead to decreased yields and the formation of off flavours in wine made from infected grapes. To assist in timely vineyard management that minimises yield and quality losses, decision support tools that correlate early detection of Botrytis infection and quantification of potential off flavour development is desirable.In this study, laboratory infection of whole bunches/ single berries with Botrytis cinerea to create a range of grey mould contamination in Shiraz, Cabernet Sauvignon, Chardonnay and Semillon were undertaken. After SPME GC-MS detection of grape homogenate, 8 out of 22 volatile compounds, including 3-octanol, 3-octanone, 1,5-dimethylnapthalene and 1,5-dimethyltetralin, were identified from VIP score and selectivity ratio, and excellent predictive model of Botrytis cinerea infection levels (determined by ergosterol measurement, antigen capture and qPCR) were developed using PLS and PLS2. These compounds, with high predictive accuracy, could be considered as potential biomarkers for rapid MS techniques in early stage.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Jiang Liang1, Qiu Y.1,2, Dumlao M.1,2,3, Donald W. A.4, Steel C. C.1,2 and Schmidtke L. M.1,2,3

1School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science, Charles Sturt University
2Gulbali Institute (Agriculture Water Environment), Charles Sturt University
3The Australian Research Council Training Centre for Innovative Wine Production, University of Adelaide
4School of Chemistry, Faculty of Science, University of New South Wales

Contact the author

Keywords

Grape disease, Grey mould, Botrytis cinerea, SPME GC-MS, Volatile organic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Influence of toasting oak wood on ellagitannin structures

Ellagitannins (ETs) have been reported to be the main phenolic compounds found in oak wood. These compounds, belonging to the hydrolysable tannin class of polyphenols, are esters of hexahydroxydiphenic acid (HHDP) and a polyol, usually glucose or quinic acid. They own their name to their capacity to be hydrolysed and liberate ellagic acid and they have an impact on astringency and bitterness sensation, which is strongly dependant on their structure. The toasting phase is particularly crucial in barrels fabrication and influences wood composition.

Read More

Using remotely sensed (UAV) and in situ field measurements to describe grapevine canopy characteristics

Row orientation and canopy management are essential for high quality grapevine production. Microclimatic conditions of the leaves and fruits can be influenced by the canopy geometry. Remote sensing is a very promising tool to describe vegetative growth and physiological behavior of vineyards. However, the correlation between remotely sensed data and in situ field measurements has been described scarcely in the scientific literature so far. The aim of the study was to correlate remotely sensed data obtained with Unmanned Aerial Vehicle (UAV) with in situ field measurements to describe canopy structure.

Read More

Approaches for estimating the age of old vineyards in Campo de Borja

Determining the age of a vineyard is essential for understanding its influence on wine quality and characteristics.

Read More

Crowdsourced the assessment of wine rating: professional wine competition rating vs vivino rating

We evaluate wine ratings by comparing data from two crowdsourcing platforms – Vivino, which aggregates the opinions of a large number of wine lovers, and Global Wine Medal Rating, which aggregates the scores from more than 1030 international wine competitions since 2020.

Read More

Partial rootzone drying (PRD): strategic irrigation management as viticultural tool affecting plant physiology and berry quality

Partial rootzone drying (PRD) is an irrigation management technique designed to reduce water use in grapevines without a decline in yield, thereby increasing water use efficiency (WUE). The principle of PRD is to keep part of the root system at a constant drying rate to produce soil derived signals to above-ground plant organs to induce a

Read More