IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Abstract

Grapes infected with grey mould due Botrytis cinerea are widespread in vineyards during certain growing conditions.  Excessive infection levels may lead to decreased yields and the formation of off flavours in wine made from infected grapes. To assist in timely vineyard management that minimises yield and quality losses, decision support tools that correlate early detection of Botrytis infection and quantification of potential off flavour development is desirable.In this study, laboratory infection of whole bunches/ single berries with Botrytis cinerea to create a range of grey mould contamination in Shiraz, Cabernet Sauvignon, Chardonnay and Semillon were undertaken. After SPME GC-MS detection of grape homogenate, 8 out of 22 volatile compounds, including 3-octanol, 3-octanone, 1,5-dimethylnapthalene and 1,5-dimethyltetralin, were identified from VIP score and selectivity ratio, and excellent predictive model of Botrytis cinerea infection levels (determined by ergosterol measurement, antigen capture and qPCR) were developed using PLS and PLS2. These compounds, with high predictive accuracy, could be considered as potential biomarkers for rapid MS techniques in early stage.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Jiang Liang1, Qiu Y.1,2, Dumlao M.1,2,3, Donald W. A.4, Steel C. C.1,2 and Schmidtke L. M.1,2,3

1School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science, Charles Sturt University
2Gulbali Institute (Agriculture Water Environment), Charles Sturt University
3The Australian Research Council Training Centre for Innovative Wine Production, University of Adelaide
4School of Chemistry, Faculty of Science, University of New South Wales

Contact the author

Keywords

Grape disease, Grey mould, Botrytis cinerea, SPME GC-MS, Volatile organic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Hyperspectral imaging for the appraisal of varietal aroma composition along maturation in intact Vitis vinifera L. Tempranillo Blanco berries

The knowledge of the grape aromatic composition during ripening provides very important information for winegrowers, who may carry out different viticultural practices, or determine the harvest date more accurately. However, there are currently no tools that allow this measurement to be carried out in a non-invasive and rapid way. For this reason, the aim of this work was to design a non-invasive methodology, based on hyperspectral imaging to estimate the aromatic composition and total soluble solids (TSS) of Tempranillo Blanco berries during ripening.

Read More

Everything else, it’s work ”Socio-cultural dimensions of terroir among Bordeaux winemakers

In 2010, the OIV adopted a resolution that defines ‘terroir’. The OIV definition understands terroir as the result of the interactions between the physical specificities of a space and human labor, with an emphasis on the subsequently produced collective knowledge (OIV-VITI 333-2010); by doing so, it alludes to the social and cultural dimensions of terroir.

Read More

A Viticultural Terroir in Brazil: Change and continuity

The viticultural terroir at the Serra Gaúcha region, in Rio Grande do Sul State, Brazil, is analyzed under historical and sociological viewpoints, aiming to understand the origin of its characteristics, and the risks for its continuity.

Read More

Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

AIM: Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, wines with low levels of quality compounds.

Read More

NMR approach for monitoring the photo-degradation of riboflavin and methionine

The light exposure of white wine is responsible for several reactions leading to changes on colour, flavours and, consequently, affecting the sensory profile.

Read More