IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Abstract

Grapes infected with grey mould due Botrytis cinerea are widespread in vineyards during certain growing conditions.  Excessive infection levels may lead to decreased yields and the formation of off flavours in wine made from infected grapes. To assist in timely vineyard management that minimises yield and quality losses, decision support tools that correlate early detection of Botrytis infection and quantification of potential off flavour development is desirable.In this study, laboratory infection of whole bunches/ single berries with Botrytis cinerea to create a range of grey mould contamination in Shiraz, Cabernet Sauvignon, Chardonnay and Semillon were undertaken. After SPME GC-MS detection of grape homogenate, 8 out of 22 volatile compounds, including 3-octanol, 3-octanone, 1,5-dimethylnapthalene and 1,5-dimethyltetralin, were identified from VIP score and selectivity ratio, and excellent predictive model of Botrytis cinerea infection levels (determined by ergosterol measurement, antigen capture and qPCR) were developed using PLS and PLS2. These compounds, with high predictive accuracy, could be considered as potential biomarkers for rapid MS techniques in early stage.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Jiang Liang1, Qiu Y.1,2, Dumlao M.1,2,3, Donald W. A.4, Steel C. C.1,2 and Schmidtke L. M.1,2,3

1School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science, Charles Sturt University
2Gulbali Institute (Agriculture Water Environment), Charles Sturt University
3The Australian Research Council Training Centre for Innovative Wine Production, University of Adelaide
4School of Chemistry, Faculty of Science, University of New South Wales

Contact the author

Keywords

Grape disease, Grey mould, Botrytis cinerea, SPME GC-MS, Volatile organic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Pinot noir: an endemic or a flexible variety?

Pinot noir has its historical roots in Burgundy and is generally considered as an endemic vine variety which means that its adaptation is very specific to this environment

Read More

Vitis v. corvina grapes composition and wine sensory profile as affected by different post harvest withering conditions

Context and purpose of the study – In Valpolicella area (Verona – Italy) Vitis vinifera cv. Corvina is the main wine variety to obtain, after grape withering, Amarone wine: this study was carried out in order to compare two different grape dehydration conditions with the aim of verifying the final composition of Corvina dried grapes and the organoleptic profile of corresponding Amarone wine.

Read More

Evaluation of state of vineyards and characterization of vineyard sites of the integrated area of Tokaj Kereskedőház ltd. in Tokaj region

The Tokaj Kereskedőház Ltd. is the only state owned winery in Hungary. The company is integrating grapes for wine production from 1100 hectares of vineyard, which consist of 3500 parcels with average size of 0,3 hectares, owned by about 500 families of the region. The vineyards are unevenly spread in total 27 village of Tokaj region.

Read More

Study of intramolecular distribution of hydrogen isotopes in ethanol depending on deuterium content of water and the origin of carbohydrates

The paper presents the results of consistently developing studies carried out in 2022-2024 on the distribution of deuterium 2H(D) in intracellular water of grapes and wine products, taking into account the influence of natural, climatic and technogenic factors using high-resolution quantitative nuclear magnetic resonance spectroscopy 2H(D)-qNMR.

Read More

Herbicide-free systems based on under-the-row grass cover in French vineyards

In a context of reducing herbicide use, the most part of French vineyards are developing permanent grass cover crops on inter-rows alleys, while under the row chemical weeding remains the general case. The setting up of a controlled grass cover crop under the vine row could be a complementary alternative to mechanical weeding – which one is very restrictive – interesting from a technical and economical point of view. The present study aimed at assessing agronomic impacts of grass cover crop under the row in different climatic conditions and production objectives.

Read More