IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Volatile analysis of Botrytis contaminated grapes using headspace solid phase microextraction GC-MS

Abstract

Grapes infected with grey mould due Botrytis cinerea are widespread in vineyards during certain growing conditions.  Excessive infection levels may lead to decreased yields and the formation of off flavours in wine made from infected grapes. To assist in timely vineyard management that minimises yield and quality losses, decision support tools that correlate early detection of Botrytis infection and quantification of potential off flavour development is desirable.In this study, laboratory infection of whole bunches/ single berries with Botrytis cinerea to create a range of grey mould contamination in Shiraz, Cabernet Sauvignon, Chardonnay and Semillon were undertaken. After SPME GC-MS detection of grape homogenate, 8 out of 22 volatile compounds, including 3-octanol, 3-octanone, 1,5-dimethylnapthalene and 1,5-dimethyltetralin, were identified from VIP score and selectivity ratio, and excellent predictive model of Botrytis cinerea infection levels (determined by ergosterol measurement, antigen capture and qPCR) were developed using PLS and PLS2. These compounds, with high predictive accuracy, could be considered as potential biomarkers for rapid MS techniques in early stage.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Jiang Liang1, Qiu Y.1,2, Dumlao M.1,2,3, Donald W. A.4, Steel C. C.1,2 and Schmidtke L. M.1,2,3

1School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science, Charles Sturt University
2Gulbali Institute (Agriculture Water Environment), Charles Sturt University
3The Australian Research Council Training Centre for Innovative Wine Production, University of Adelaide

4School of Chemistry, Faculty of Science, University of New South Wales

Contact the author

Keywords

Grape disease, Grey mould, Botrytis cinerea, SPME GC-MS, Volatile organic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Bees, climate changes, and “environmental sustainability 4.1c” in viticulture and the territory for a new global multiproductive “biometaethical district 4.1c”

The use of bees as pollinators in vine varieties with physiologically female flowers (Picolit, Bicane, Ceresa, Moscato rosa, etc.) (Cargnello, 1983) and as bio-indicators for biodiversity and environmental sustainability is well-known. Furthermore, there are interests in: 1-a. Making the viticulture of Belluno (Province of Veneto in North-eastern Italy, which is also famous for the Dolomites -a UNESCO World Heritage-) regain the socioeconomic role which it is entitled to and which it had got in its past by aiming at the enhancement of local grape variety in harmony with others, for example with the neighboring area of the Conegliano and Valdobbiadene Prosecco Superiore DOCG; 2-a. Maintaining and further improving the important natural and healthy environment of Belluno, and making its territory and the “lookout” means of the environmental sustainability, including its vineyards, even more naturally original and sustainable 4.1C.

Sensory analysis in oenology: the role of methodological differences in expert panel evaluations

Sensory analysis is an essential component of oenology, offering valuable insights into wine quality that influence decision-making in viticulture and winemaking.

Novel analytical technologies for wine fingerprinting in and beyond the laboratory

For characterization, sensory designing and authentication rapid analytical technologies have become available. Some, like Proton Transfer Reaction Mass Spectrometry allow a rapid spectrum of the volatile compounds of wines. Combined with chemometrics wines can be characterized. The same approach can be used to calculate the results of virtual mixtures and allow formulation of constant quality blends. Other new techniques and portable devices based on spectroscopy allow measurements on production sites and in grocery stores, even for the smart consumer. We will present some examples of the application of these techniques for authentication of wines, both in the laboratory and on site.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Investigating three proximal remote sensing techniques for vineyard yield monitoring

Yield monitoring can provide the winegrowers with information for precise production inputs during the season, thereby, ensuring the best possible harvest. Yield estimation is currently achieved through an intensive process that is destructive and time-consuming. However, remote sensing provides a group of proximal technologies and techniques for a non-destructive and less time-consuming method for yield monitoring.The objective of this study was to analyse three different approaches, for measuring grapevine yield close to harvest.