IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Botrytis cinerea: Coconut or Catastrophe? Quantification of γ-Nonalactone in Botrytised and Non-Botrytised New Zealand Wines

Botrytis cinerea: Coconut or Catastrophe? Quantification of γ-Nonalactone in Botrytised and Non-Botrytised New Zealand Wines

Abstract

g-Nonalactone has been identified as a significant contributor to the aroma profile of a range of wines and is associated with stonefruit and coconut descriptors. The exact route of g-nonalactone biosynthesis in wine has not been fully elucidated; however, precursors including 4-oxononanoic acid, linoleic acid, 13-hydroxyoctadeca-9,11-dienoic acid (13-HODE) and 9-hydroxyoctadeca-10,12-dienoic acid (9-HODE) have been identified in incubation experiments. Wines produced from grapes infected with “noble rot” caused by Botrytis cinerea fungus generally show higher concentrations of g-nonalactone compared to non-botrytised white wines, but the relative contribution of potential formation pathways has not been elucidated. To assess the effect of linoleic acid on the production of g-nonalactone in wine, fermentations with and without added linoleic acid were carried out in synthetic grape must (SGM) at 28 °C using commercial Saccharomyces cerevisiae EC1118. Prior to g-nonalactone quantitation in the finished wines and in a subset of six Australian and New Zealand commercial wines, several routes for the synthesis of a deuterated analogue of g-nonalactone were attempted, before the deuterated d6-analogue of g-nonalactone from its non-deuterated analogue was produced successfully. Subsequently, attempts were made to utilise the d6-analogue as an internal standard for the measurement of g-nonalactone using gas chromatography-mass spectrometry. However, the synthetic deuterated g-nonalactone analogue proved to be an inappropriate internal standard for this purpose, due to incomplete incorporation of deuterium atoms. 2-Octanol was instead used as a surrogate internal standard. g-Nonalactone was successfully identified (above the limit of detection, 4.12g/L) in two commercial New Zealand botrytised wine samples, and one fermentation sample to which linoleic acid (132 mg/L) had been added. This suggests a possible link between the effect of Botrytis cinerea and/or linoleic acid, and increased levels of g-nonalactone in wine. The promising results in these preliminary experiments have led to an improved internal standard being sought out for the quantification of g-nonalactone in wine, and further investigation of its biosynthesis. A 13C4-labelled g-nonalactone analogue was successfully synthesized based on a previous method, with four 13C atoms being introduced into the molecule via two Wittig olefination steps. This standard will be used for a much larger survey of approximately 40 botrytised and non-botrytised New Zealand wines, in addition to further fermentation experiments assessing the effects of the addition of a wider range of putative precursors.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Miller Gillean1, Fedrizzi Bruno1, Barker David1, Pilkington Lisa1 and Deed Rebecca1

1The University of Auckland, School of Chemical Sciences

Contact the author

Keywords

Lactones, Botrytis, White wine, New Zealand, GC-MS

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.