IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Botrytis cinerea: Coconut or Catastrophe? Quantification of γ-Nonalactone in Botrytised and Non-Botrytised New Zealand Wines

Botrytis cinerea: Coconut or Catastrophe? Quantification of γ-Nonalactone in Botrytised and Non-Botrytised New Zealand Wines

Abstract

g-Nonalactone has been identified as a significant contributor to the aroma profile of a range of wines and is associated with stonefruit and coconut descriptors. The exact route of g-nonalactone biosynthesis in wine has not been fully elucidated; however, precursors including 4-oxononanoic acid, linoleic acid, 13-hydroxyoctadeca-9,11-dienoic acid (13-HODE) and 9-hydroxyoctadeca-10,12-dienoic acid (9-HODE) have been identified in incubation experiments. Wines produced from grapes infected with “noble rot” caused by Botrytis cinerea fungus generally show higher concentrations of g-nonalactone compared to non-botrytised white wines, but the relative contribution of potential formation pathways has not been elucidated. To assess the effect of linoleic acid on the production of g-nonalactone in wine, fermentations with and without added linoleic acid were carried out in synthetic grape must (SGM) at 28 °C using commercial Saccharomyces cerevisiae EC1118. Prior to g-nonalactone quantitation in the finished wines and in a subset of six Australian and New Zealand commercial wines, several routes for the synthesis of a deuterated analogue of g-nonalactone were attempted, before the deuterated d6-analogue of g-nonalactone from its non-deuterated analogue was produced successfully. Subsequently, attempts were made to utilise the d6-analogue as an internal standard for the measurement of g-nonalactone using gas chromatography-mass spectrometry. However, the synthetic deuterated g-nonalactone analogue proved to be an inappropriate internal standard for this purpose, due to incomplete incorporation of deuterium atoms. 2-Octanol was instead used as a surrogate internal standard. g-Nonalactone was successfully identified (above the limit of detection, 4.12g/L) in two commercial New Zealand botrytised wine samples, and one fermentation sample to which linoleic acid (132 mg/L) had been added. This suggests a possible link between the effect of Botrytis cinerea and/or linoleic acid, and increased levels of g-nonalactone in wine. The promising results in these preliminary experiments have led to an improved internal standard being sought out for the quantification of g-nonalactone in wine, and further investigation of its biosynthesis. A 13C4-labelled g-nonalactone analogue was successfully synthesized based on a previous method, with four 13C atoms being introduced into the molecule via two Wittig olefination steps. This standard will be used for a much larger survey of approximately 40 botrytised and non-botrytised New Zealand wines, in addition to further fermentation experiments assessing the effects of the addition of a wider range of putative precursors.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Miller Gillean1, Fedrizzi Bruno1, Barker David1, Pilkington Lisa1 and Deed Rebecca1

1The University of Auckland, School of Chemical Sciences

Contact the author

Keywords

Lactones, Botrytis, White wine, New Zealand, GC-MS

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Pruned vine biomass exclusion from a clay loam vineyard soil – examining the impact on physical/chemical properties

The wine industry worldwide faces increasing challenges to achieve sustainable levels of carbon emission mitigation. This project seeks to establish the feasibility of harvesting winter pruned vineyard biomass (PVB) for potential use in carbon footprint reduction, through its use as a renewable biofuel for energy production. In order to make this recommendation, technical issues such as the potential environmental impact, chemical composition and fuel suitability, and logistical challenges of harvesting biomass needs to be understood to compare with the results from similar studies. Of particular interest is the role PVB plays as a carbon source in vineyard soils and what effect annual removal might have on soil carbon sequestration. A preliminary trial was established in the Waite Campus vineyard (University of Adelaide) to test current management strategies. Vines are grown in a Eutrophic, Red Dermosol clay loam soil with well managed midrow swards. A comparison was undertaken of mid-row treatments in two 0.25 Ha blocks (Shiraz and Semillon), including annual cultivation for seed bed preparation, the deliberate exclusion of PVB (25 years) and incorporation of PVB (13 years) at an average of 3.4 and 5.5 Mg/Ha-1 for Shiraz and Semillon respectively. In both 0-10cm and 10-30cm soil core sample depths, combined soil carbon % measures in the desired range of 1.80 to 3.50, were not significantly different between treatments or cultivars and yielded an estimated 42 Mg/ha-1 of sequestered soil carbon. Other key physical and chemical measures were likewise not significantly different between treatments. Preliminary results suggest that in a temperate zone vineyard, managed such as the one used in this study, there is no long term negative impact on soil carbon sequestration through removing PVB. This implies that growers could confidently harvest PVB for use in several end fates including as a bio fuel.

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.