IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Varietal thiol precursors in Trebbiano di Lugana grape and must

Varietal thiol precursors in Trebbiano di Lugana grape and must

Abstract

Trebbiano di Lugana (TdL) is a white variety of Vitis vinifera mainly cultivated in an Italian area located south near Garda lake (Verona, north of Italy). This grape cultivar, also known as “Turbiana,” is used for the production of TdL wine with recognized Protected Designation of Origin whose volatile profile was recently determined [1]. The presence of varietal thiols in TdL, namely 3-mercaptohexan-1-ol and its acetate form, conferring the tropical and citrus notes, has been documented. Winemaking strategies were also described with the purpose of protecting and maintain these desired aromas [2]. To the best of our knowledge, the varietal thiol precursors (VTPs) were not previously determined in TdL grape and must. This study aimed to quantify VTPs in both grape during the ripening and must during the pressing. Volatile C6 compounds were also measured in the must fractions.
TdL grapes were sampled during the ripening in two vineyards, differing for the content of readily assimilable nitrogen (RAN), for a total of five samplings each. The musts were produced in an industrial plan collecting the samples during the pressing for a total of nine samplings [3]. VTPs were identified and quantified in grape fractions, grape samples and must fractions by ULPC coupled High Resolution Mass Spectrometry (HRMS) after SPE of samples [4]. Volatile C6 compounds, namely trans-2-hexen-1-ol, trans-3-hexen-ol, 2-hexenal, 1-hexanol, cis-3-hexen-1-ol and cis-2-hexen-1-ol, were determined by SPME-GC/MS [5].
S-3-(hexan-1-ol)-L-glutathione (G-3SH), S-3-(hexan-1-ol)-L-cysteine (Cys-3SH) and S-3-(hexanal)-glutathione (G-3SHal) were detected in both grape and must samples. At harvest, grapes with lower RAN revealed about 3-folds lower levels of G-3SH (79.71±0.97 μg/L vs. 208.66±1.35 μg/L) and G-3SHal (4.7±0.1 mg/L vs. 13.1±0.0 mg/L), and 2-folds lower amounts of Cys-3SH (11.95±0.82 μg/L vs. 21.75±0.47 μg/L). This suggests the level of RAN in grape to affect VPT synthesis. Nonetheless, the musts obtained with the two grapes showed comparable concentrations of G-3SH (50.71±0.37 μg/L as average); Cys-3SH was found at trace levels in both musts, and little amounts of G-3SHal was detected only in the must with higher RAN (29.53±7.37 μg/L). Considering the volatile C6 compounds, trans-3-hexen-1-ol, cis-3-hexen-1-ol and cis-2-hexen-1-ol were similar in the two investigated musts; trans-2-hexen-1-ol was higher in the must with low RAN as well as 2-hexenal and 1-hexanol. No significant correlation was found between the VPTs and volatile C6 compounds in the must fractions analysed.
These data suggest RAN to impact the VPT concentrations in grape. Moreover, pressing was found to play an important role on VPTs content of musts.

References

[1] Fracassetti D., Camoni D., Montresor L., Bodon R., Limbo S. Chemical characterization and volatile profile of Trebbiano di Lugana wine: A case study. Foods 2020, 9, 956. https://doi.org/10.3390/foods9070956.
[2] Mattivi F., Fedrizzi B., Zenato A., Tiefenthaler P., Tempesta S., Perenzoni D., Cantarella P., Simeoni F., Vrhovsek U. Development of reliable analytical tools for evaluating the influence of reductive winemaking on the quality of Lugana wines. Anal. Chim. Acta 2012, 732, 194–202. https://doi.org/10.1016/j.aca.2011.11.051.
[3] Tirelli A., De Noni I., Stuknytė M., Pica V., Fracassetti D. Role of extraction procedures on the concentration of varietal thiol precursors in Grillo white grape must. Aust. J. Grape Wine Res. 2022, 28, 61-69. https://doi.org/10.1111/ajgw.12514.
[4] Fracassetti D., Stuknyté M., La Rosa C., Gabrielli M., De Noni I., Tirelli A. Thiol precursors in Catarratto Bianco Comune and Grillo grapes and effect of clarification conditions on the release of varietal thiols in wine. Aust. J. Grape Wine Res. 2018, 24, 125-133. https://doi.org/10.1111/ajgw.12311.
[5] Bosso A., Follis R., Guaita M., Motta S., Panero L., Petrozziello M. Caratterizzazione del quadro polifenolico ed aromatico di mosti di 5 diverse cultivar a bacca bianca, sottoposti a pressatura all’aria a sotto azoto. From “Territori di vini-progetti di ricerca per il settore vitivinicolo” edited by Società Consortile territori Divini A.R.L. (stampa La GRAFICA FAGGIAN S.R.L.-Campodarsego (PD). Proceedings of the conference “Territori diVini”, Treviso, 24 june 2011: 29-37.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Fracassetti Daniela1, De Noni Ivano1, Petrozziello Maurizio2, Bonello Frederica2 and Tirelli Antonio1 

1Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano
2CREA-VE Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria – Centro di Ricerca Viticoltura ed Enologia

Contact the author

Keywords

Grape ripening, Pressing, C6 compounds, Must

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Use of multispectral satellite for monitoring vine water status in mediterranean areas

The development of new generations of multispectral satellites such as Sentinel-2 opens possibilities as to vine water status assessment (Cohen et al., 2019). Based on a three years field campaign, a model of Stem Water Potential (SWP) estimation on vine using four satellite bands in Red, Red-Edge, NIR and SWIR domains was developed (Laroche-Pinel et al., 2021). The model relies on SWP field measures done using a pressure chamber (Scholander et al., 1965), which is a common, robust and precise method to assess vine water status (Acevedo-Opazo et al., 2008). The model was mainly developed from from SWP measures on Syrah N (Laroche Pinel E., 2021). A large scale monitoring was organized in different vineyards in the Mediterranean region in 2021. 10 varieties amongst the most represented in this area were monitored (Cabernet sauvignon N, Chardonnay B, Cinsault N, Grenache N, Merlot N, Mourvèdre N, Sauvignon B, Syrah N, Vermentino B, Viognier B). The model was used to produce water status maps from Sentinel-2 images, starting from the beginning of June (fruit set) up to September (harvest). The average estimated SWP for each vine was compared to actual field SWP measures done by wine growers or technicians during usual monitoring of irrigation programs. The correlations between mean estimated SWP and mean measured SWP were at the same level than expected by the model. (Laroche Pinel, 2021) The general SWP kinetics were comparable. The estimated SWP would have led to same irrigation decisions concerning the date of first irrigation in comparison with measured SWP. Acevedo-Opazo, C., Tisseyre, B., Ojeda, H., Ortega-Farias, S., Guillaume, S. (2008). Is it possible to assess the spatial variability of vine water status? OENO One, 42(4), 203. Cohen, Y., Gogumalla, P., Bahat, I., Netzer, Y., Ben-Gal, A., Lenski, I., … Helman, D. (2019). Can time series of multispectral satellite images be used to estimate stem water potential in vineyards? In Precision agriculture ’19, The Netherlands: Wageningen Academic Publishers, pp. 445–451. Laroche-Pinel, E., Duthoit, S., Albughdadi, M., Costard, A. D., Rousseau, J., Chéret, V., & Clenet, H. (2021). Towards vine water status monitoring on a large scale using sentinel-2 images. remote sensing, 13(9), 1837. Laroche-Pinel,E. (2021). Suivi du statut hydrique de la vigne par télédétection hyper et multispectrale. Thèse INP Toulouse, France. Scholander, P.F., Bradstreet, E.D., Hemmingsen, E.A., & Hammel, H.T. (1965). Sap pressure in vascular plants: Negative hydrostatic pressure can be measured in plants. Science, 148(3668), 339–346.

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Local ancient grapevine cultivars to face future viticulture

Among the different strategies to cope with the negative impacts of climate change on viticulture, the exploitation of genetic diversity is one of the most promising to adapt to new conditions and maintain wine production and quality. One of the biggest concerns in the context of climate change is to improve water use efficiency (WUE). In this way, the use of genotypes that present a better response to drought and high WUE is a key issue. In this work, physiological performance analysis was conducted to compare the water deficit stress (WDS) responses of local and widespread grapevines cultivars. Leaf gas exchange, water use efficiency (WUE) at different levels (leaf and long-term WUE (∆13C)), leaf osmotic adjustment and other water relations parameters were determined in plants under well-watered and WDS conditions alongside assessment of the levels of foliar hormones concentrations. Results denote that local cultivars displayed better physiological performance under WDS as compared to the widely-distributed ones. he results corroborate the hypothesis that better stomatal control allows increasing leaf WUE under drought as occurred in the local Callet cv.; but the minority local cultivar Escursac cv. showed high WUE under both treatments. In this case, high WUE can be related to maintaining higher photosynthetic activity under drought. The different mechanisms underlying the better performance under WDS and high WUE of minority local cultivars are discussed.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Elevational range shifts of mountain vineyards: Recent dynamics in response to a warming climate

Increasing temperatures worldwide are expected to cause a change in spatial distribution of plant species along elevational gradients and there are already observable shifts to higher elevations as a consequence of climate change for many species. Not only naturally growing plants, but also agricultural cultivations are subject to the effects of climate change, as the type of cultivation and the economic viability depends largely on the prevailing climatic conditions. A shift to higher elevations therefore represents a viable adaptation strategy to climate change, as higher elevations are characterized by lower temperatures. This is especially important in the case of viticulture because a certain wine-style can only be achieved under very specific climatic conditions. Although there are several studies investigating climatic suitability within winegrowing regions or longitudinal shifts of winegrowing areas, little is known about how fast vineyards move to higher elevations, which may represent a viable strategy for winegrowers to maintain growing conditions and thus wine-style, despite the effects of climate change. We therefore investigated the change in the spatial distribution of vineyards along an elevational gradient over the past 20 years in the mountainous wine-growing region of Alto Adige (Italy). A dataset containing information about location and planting year of more than 26000 vineyard parcels and 30 varieties was used to perform this analysis. Preliminary results suggest that there has been a shift to higher elevations for vineyards in general (from formerly 700m to currently 850 m a.s.l., with extreme sites reaching 1200 m a.s.l.), but also that this development has not been uniform across different varieties and products (i.e. vitis vinifera vs hybrid varieties and still vssparkling wines). This is important for climate change adaptation as well as for rural development. Mountain areas, especially at mid to high elevations, are often characterized by severe land abandonment which can be avoided to some degree if economically viable and sustainable land management strategies are available.