IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Varietal thiol precursors in Trebbiano di Lugana grape and must

Varietal thiol precursors in Trebbiano di Lugana grape and must

Abstract

Trebbiano di Lugana (TdL) is a white variety of Vitis vinifera mainly cultivated in an Italian area located south near Garda lake (Verona, north of Italy). This grape cultivar, also known as “Turbiana,” is used for the production of TdL wine with recognized Protected Designation of Origin whose volatile profile was recently determined [1]. The presence of varietal thiols in TdL, namely 3-mercaptohexan-1-ol and its acetate form, conferring the tropical and citrus notes, has been documented. Winemaking strategies were also described with the purpose of protecting and maintain these desired aromas [2]. To the best of our knowledge, the varietal thiol precursors (VTPs) were not previously determined in TdL grape and must. This study aimed to quantify VTPs in both grape during the ripening and must during the pressing. Volatile C6 compounds were also measured in the must fractions.
TdL grapes were sampled during the ripening in two vineyards, differing for the content of readily assimilable nitrogen (RAN), for a total of five samplings each. The musts were produced in an industrial plan collecting the samples during the pressing for a total of nine samplings [3]. VTPs were identified and quantified in grape fractions, grape samples and must fractions by ULPC coupled High Resolution Mass Spectrometry (HRMS) after SPE of samples [4]. Volatile C6 compounds, namely trans-2-hexen-1-ol, trans-3-hexen-ol, 2-hexenal, 1-hexanol, cis-3-hexen-1-ol and cis-2-hexen-1-ol, were determined by SPME-GC/MS [5].
S-3-(hexan-1-ol)-L-glutathione (G-3SH), S-3-(hexan-1-ol)-L-cysteine (Cys-3SH) and S-3-(hexanal)-glutathione (G-3SHal) were detected in both grape and must samples. At harvest, grapes with lower RAN revealed about 3-folds lower levels of G-3SH (79.71±0.97 μg/L vs. 208.66±1.35 μg/L) and G-3SHal (4.7±0.1 mg/L vs. 13.1±0.0 mg/L), and 2-folds lower amounts of Cys-3SH (11.95±0.82 μg/L vs. 21.75±0.47 μg/L). This suggests the level of RAN in grape to affect VPT synthesis. Nonetheless, the musts obtained with the two grapes showed comparable concentrations of G-3SH (50.71±0.37 μg/L as average); Cys-3SH was found at trace levels in both musts, and little amounts of G-3SHal was detected only in the must with higher RAN (29.53±7.37 μg/L). Considering the volatile C6 compounds, trans-3-hexen-1-ol, cis-3-hexen-1-ol and cis-2-hexen-1-ol were similar in the two investigated musts; trans-2-hexen-1-ol was higher in the must with low RAN as well as 2-hexenal and 1-hexanol. No significant correlation was found between the VPTs and volatile C6 compounds in the must fractions analysed.
These data suggest RAN to impact the VPT concentrations in grape. Moreover, pressing was found to play an important role on VPTs content of musts.

References

[1] Fracassetti D., Camoni D., Montresor L., Bodon R., Limbo S. Chemical characterization and volatile profile of Trebbiano di Lugana wine: A case study. Foods 2020, 9, 956. https://doi.org/10.3390/foods9070956.
[2] Mattivi F., Fedrizzi B., Zenato A., Tiefenthaler P., Tempesta S., Perenzoni D., Cantarella P., Simeoni F., Vrhovsek U. Development of reliable analytical tools for evaluating the influence of reductive winemaking on the quality of Lugana wines. Anal. Chim. Acta 2012, 732, 194–202. https://doi.org/10.1016/j.aca.2011.11.051.
[3] Tirelli A., De Noni I., Stuknytė M., Pica V., Fracassetti D. Role of extraction procedures on the concentration of varietal thiol precursors in Grillo white grape must. Aust. J. Grape Wine Res. 2022, 28, 61-69. https://doi.org/10.1111/ajgw.12514.
[4] Fracassetti D., Stuknyté M., La Rosa C., Gabrielli M., De Noni I., Tirelli A. Thiol precursors in Catarratto Bianco Comune and Grillo grapes and effect of clarification conditions on the release of varietal thiols in wine. Aust. J. Grape Wine Res. 2018, 24, 125-133. https://doi.org/10.1111/ajgw.12311.
[5] Bosso A., Follis R., Guaita M., Motta S., Panero L., Petrozziello M. Caratterizzazione del quadro polifenolico ed aromatico di mosti di 5 diverse cultivar a bacca bianca, sottoposti a pressatura all’aria a sotto azoto. From “Territori di vini-progetti di ricerca per il settore vitivinicolo” edited by Società Consortile territori Divini A.R.L. (stampa La GRAFICA FAGGIAN S.R.L.-Campodarsego (PD). Proceedings of the conference “Territori diVini”, Treviso, 24 june 2011: 29-37.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Fracassetti Daniela1, De Noni Ivano1, Petrozziello Maurizio2, Bonello Frederica2 and Tirelli Antonio1 

1Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano
2CREA-VE Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria – Centro di Ricerca Viticoltura ed Enologia

Contact the author

Keywords

Grape ripening, Pressing, C6 compounds, Must

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.

Assessing the climate change vulnerability of European winegrowing regions by combining exposure, sensitivity and adaptive capacity indicators

Winegrowing regions recognized as protected designations of origin (PDOs) are closely tied to well defined geographic locations with a specific set of pedoclimatic attributes and strictly regulated by legal specifications. However, climate change is increasingly threatening these regions by changing local conditions and altering winegrowing processes. The vulnerability to these changes is largely heterogenous across different winegrowing regions because it is determined by individual characteristics of each region, including the capacity to adapt to new climatic conditions and the sensitivity to climate change, which depend not only on natural, but also socioeconomic and legal factors. Accurate vulnerability assessments therefore need to combine information about adaptive capacity and climate change sensitivity with projected exposure to new climatic conditions. However, most existing studies focus on specific impacts neglecting important interactions between the different factors that determine climate change vulnerability. Here, we present the first comprehensive vulnerability assessment of European wine PDOs that spatially combines multiple indicators of adaptive capacity and climate change sensitivity with high-resolution climate projections. We found that the climate change vulnerability of PDO areas largely depends on the complex interactions between physical and socioeconomic factors. Homogenous topographic conditions and a narrow varietal spectrum increase climate change vulnerability, while the skills and education of farmers, together with a good economic situation, decrease their vulnerability. Assessments of climate change consequences therefore need to consider multiple variables as well as their interrelations to provide a comprehensive understanding of the expected impacts of climate change on European PDOs. Our results provide the first vulnerability assessment for European winegrowing regions at high spatiotemporal resolution that includes multiple factors related to climate exposure, sensitivity, and adaptive capacity on the level of single winegrowing regions. They will therefore help to identify hot spots of climate change vulnerability among European PDOs and efficiently direct adaptation strategies.

Combining effect of leaf removal and natural shading on grape ripening under two irrigation strategies in Manto negro (Vitis vinifera L.)

The increasingly frequent heat waves during grape ripening pose challenges for high quality wine grape production. Defoliation is a common practice that can improve the control of diseases in bunches, but also it increases the exposure to sunlight. Grapes exposed to solar radiation reach temperatures over the optimum for berry development and maturation. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 using Manto negro wine grapes to study the effect of applied irrigation and different light exposure levels on grape quality. Two irrigation treatments were imposed based on the frequency and amount of water doses in a four-block experimental vineyard at Bodega Ribas (Mallorca). Three light exposure treatments were randomly applied in each irrigation plot. The light treatments included exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening. Leaf area index and canopy porosity was estimated every 2 weeks. Midday leaf water potential was measured weekly. Additionally, apparent electrical conductivity was measured between rows to estimate the soil water content variability. Light and temperature sensors were installed at the bunch level to quantify the differences in bunch temperature and light intensity among treatments. The effect of irrigation and cluster light exposure on berry weight, TSS, TA, malic acid, tartaric acid, K+, and pH were analysed at 5 moments along grape ripening. During different heat waves, the natural shading technique decreased the maximum bunch temperature around 10 °C respect to the exposed bunches in both irrigation strategies. The combination of defoliation and shading techniques after softening decreased TSS at harvest and affected most of the quality parameters during the last stages of ripening, showing an interesting technique to delay ripening in warm viticulture areas.

Teasing apart terroir: the influence of management style on native yeast communities within Oregon wineries and vineyards

Newer sequencing technologies have allowed for the addition of microbes to the story of terroir. The same environmental factors that influence the phenotypic expression of a crop also shape the composition of the microbial communities found on that crop. For fermented goods, such as wine, that microbial community ultimately influences the organoleptic properties of the final product that is delivered to customers. Recent studies have begun to study the biogeography of wine-associated microbes within different growing regions, finding that communities are distinct across landscapes. Despite this new knowledge, there are still many questions about what factors drive these differences. Our goal was to quantify differences in yeast communities due to management style between seven pairs of conventional and biodynamic vineyards (14 in total) throughout Oregon, USA. We wanted to answer the following questions: 1) are yeast communities distinct between biodynamic vineyards and conventional vineyards? 2) are these differences consistent across a large geographic region? 3) can differences in yeast communities be tied to differences in metabolite profiles of the bottled wine? To collect our data we took soil, bark, leaf, and grape samples from within each vineyard from five different vines of pinot noir. We also collected must and a 10º brix sample from each winery. Using these samples, we performed 18S amplicon sequencing to identify the yeast present. We then used metabolomics to characterize the organoleptic compounds present in the bottled wine from the blocks the year that we sampled. We are actively in the process of analysing our data from this study.

Effect of one-year cover crop and arbuscular mycorrhiza inocululation in the microbial soil community of a vineyard

The microbial composition of the soil is an important factor to consider in viticulture, since its influence on the “terroir” and on the organoleptic properties of the wine have been demonstrated. Different agronomic techniques have the potential to modify the composition and functionality of the soil microbial community. Maintaining green covers is known to increase soil microbial diversity. The direct application of inoculum of beneficial microorganisms to the soil has also been used to increase their abundance. However, the environmental conditions of each site seem to have a determining weight in the result of these practices. In this study, we compared the effect on the microbial community of a cover crop with legumes in autumn and the inoculation of grapevines with commercial inoculum bases on Rhizophagus irregularis and Funeliformis mosseae in the previous spring. The study has been carried out in a vineyard in Binissalem, Mallorca, Spain. After applying the treatments, we will analyze the soil microbial communities using the data obtained from Illumina amplification of soil DNA from the 16S and ITS regions to analyze bacteria and fungi community, respectively. In addition, we will record the physicochemical characteristics of the soil at each sampling point. The result showed that agronomic management, in the short term, has less influence than soil characteristics on the composition of the soil microbiome. With these results, we can conclude that in a vineyard, agricultural techniques should focus on improving the characteristics of the soil to improve the biodiversity of the soil microbiota.