IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Varietal thiol precursors in Trebbiano di Lugana grape and must

Varietal thiol precursors in Trebbiano di Lugana grape and must

Abstract

Trebbiano di Lugana (TdL) is a white variety of Vitis vinifera mainly cultivated in an Italian area located south near Garda lake (Verona, north of Italy). This grape cultivar, also known as “Turbiana,” is used for the production of TdL wine with recognized Protected Designation of Origin whose volatile profile was recently determined [1]. The presence of varietal thiols in TdL, namely 3-mercaptohexan-1-ol and its acetate form, conferring the tropical and citrus notes, has been documented. Winemaking strategies were also described with the purpose of protecting and maintain these desired aromas [2]. To the best of our knowledge, the varietal thiol precursors (VTPs) were not previously determined in TdL grape and must. This study aimed to quantify VTPs in both grape during the ripening and must during the pressing. Volatile C6 compounds were also measured in the must fractions.
TdL grapes were sampled during the ripening in two vineyards, differing for the content of readily assimilable nitrogen (RAN), for a total of five samplings each. The musts were produced in an industrial plan collecting the samples during the pressing for a total of nine samplings [3]. VTPs were identified and quantified in grape fractions, grape samples and must fractions by ULPC coupled High Resolution Mass Spectrometry (HRMS) after SPE of samples [4]. Volatile C6 compounds, namely trans-2-hexen-1-ol, trans-3-hexen-ol, 2-hexenal, 1-hexanol, cis-3-hexen-1-ol and cis-2-hexen-1-ol, were determined by SPME-GC/MS [5].
S-3-(hexan-1-ol)-L-glutathione (G-3SH), S-3-(hexan-1-ol)-L-cysteine (Cys-3SH) and S-3-(hexanal)-glutathione (G-3SHal) were detected in both grape and must samples. At harvest, grapes with lower RAN revealed about 3-folds lower levels of G-3SH (79.71±0.97 μg/L vs. 208.66±1.35 μg/L) and G-3SHal (4.7±0.1 mg/L vs. 13.1±0.0 mg/L), and 2-folds lower amounts of Cys-3SH (11.95±0.82 μg/L vs. 21.75±0.47 μg/L). This suggests the level of RAN in grape to affect VPT synthesis. Nonetheless, the musts obtained with the two grapes showed comparable concentrations of G-3SH (50.71±0.37 μg/L as average); Cys-3SH was found at trace levels in both musts, and little amounts of G-3SHal was detected only in the must with higher RAN (29.53±7.37 μg/L). Considering the volatile C6 compounds, trans-3-hexen-1-ol, cis-3-hexen-1-ol and cis-2-hexen-1-ol were similar in the two investigated musts; trans-2-hexen-1-ol was higher in the must with low RAN as well as 2-hexenal and 1-hexanol. No significant correlation was found between the VPTs and volatile C6 compounds in the must fractions analysed.
These data suggest RAN to impact the VPT concentrations in grape. Moreover, pressing was found to play an important role on VPTs content of musts.

References

[1] Fracassetti D., Camoni D., Montresor L., Bodon R., Limbo S. Chemical characterization and volatile profile of Trebbiano di Lugana wine: A case study. Foods 2020, 9, 956. https://doi.org/10.3390/foods9070956.
[2] Mattivi F., Fedrizzi B., Zenato A., Tiefenthaler P., Tempesta S., Perenzoni D., Cantarella P., Simeoni F., Vrhovsek U. Development of reliable analytical tools for evaluating the influence of reductive winemaking on the quality of Lugana wines. Anal. Chim. Acta 2012, 732, 194–202. https://doi.org/10.1016/j.aca.2011.11.051.
[3] Tirelli A., De Noni I., Stuknytė M., Pica V., Fracassetti D. Role of extraction procedures on the concentration of varietal thiol precursors in Grillo white grape must. Aust. J. Grape Wine Res. 2022, 28, 61-69. https://doi.org/10.1111/ajgw.12514.
[4] Fracassetti D., Stuknyté M., La Rosa C., Gabrielli M., De Noni I., Tirelli A. Thiol precursors in Catarratto Bianco Comune and Grillo grapes and effect of clarification conditions on the release of varietal thiols in wine. Aust. J. Grape Wine Res. 2018, 24, 125-133. https://doi.org/10.1111/ajgw.12311.
[5] Bosso A., Follis R., Guaita M., Motta S., Panero L., Petrozziello M. Caratterizzazione del quadro polifenolico ed aromatico di mosti di 5 diverse cultivar a bacca bianca, sottoposti a pressatura all’aria a sotto azoto. From “Territori di vini-progetti di ricerca per il settore vitivinicolo” edited by Società Consortile territori Divini A.R.L. (stampa La GRAFICA FAGGIAN S.R.L.-Campodarsego (PD). Proceedings of the conference “Territori diVini”, Treviso, 24 june 2011: 29-37.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Fracassetti Daniela1, De Noni Ivano1, Petrozziello Maurizio2, Bonello Frederica2 and Tirelli Antonio1 

1Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano
2CREA-VE Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria – Centro di Ricerca Viticoltura ed Enologia

Contact the author

Keywords

Grape ripening, Pressing, C6 compounds, Must

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status. In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date). Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Modelling vine water stress during a critical period and potential yield reduction rate in European wine regions: a retrospective analysis

Most European vineyards are managed under rainfed conditions, where seasonal water deficit has become increasingly important. The flowering-veraison phenophase represents an important period for vine response to water stress, which is seldomly thoroughly evaluated. Therefore, we aim to quantify the flowering-veraison water stress levels using Crop Water Stress Indicator (CWSI) over 1986–2015 for important European wine regions, and to assess the respective potential Yield Lose Rate (YLR). Additionally, we also investigate whether an advanced flowering-veraison phase may help alleviating the water stress with improved yield. A process-based grapevine model STICS is employed, which has been extensively calibrated for flowering and veraison stages using observed data at 38 locations with 10 different grapevine varieties. Subsequently, the model is being implemented at the regional level, considering site-specific calibration results and gridded climate and soil datasets. The findings suggest wine regions with stronger flowering-veraison CWSI tend to have higher potential YLR. However, contrasting patterns are found between wine regions in France-Germany-Luxembourg and Italy-Portugal-Spain. The former tends to have slight-to-moderate drought conditions (CWSI<0.5) and a negligible-to-moderate YLR (<30%), whereas the latter possesses severe-to-extreme CWSI (>0.5) and substantial YLR (>40%). Wine regions prone to a high drought risk (CWSI>0.75) are also identified, which are concentrated in southern Mediterranean Europe. An advanced flowering-veraison phase may have benefited from cooler temperatures and a higher fraction of spring precipitation in wine regions of Italy-Portugal-Spain, resulting in alleviated CWSI and moderate reductions of YLR. For those of France-Germany-Luxembourg, this can have reduced flowering-veraison precipitation, but prevalent alleviations of YLR are also found, possibly because of shifted phase towards a cooler growing season with reduced evaporative demands. Overall, such a retrospective analysis might provide new insights towards better management of seasonal water deficit for conventionally vulnerable Mediterranean wine regions, but also for relatively cooler and wetter Central European regions.

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

Projected changes in vine phenology of two varieties with different thermal requirements cultivated in La Mancha DO (Spain) under climate change scenarios

The aim of this work was to analyze the phenology variability of Tempranillo and Chardonnay cultivars, related to the climatic characteristics in La Mancha Designation of Origin, and their potential changes under climate change scenarios. Phenological dates referred to budbreak, flowering, veraison and harvest were analyzed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The thermal requirements to reach each of these phenological stages were calculated and expressed as the GDD accumulated from DOY=60. Changes in phenology were projected by 2050 and 2070 taking into account those values and the projected temperatures and precipitation, simulated under two Representative Concentration Pathway (RCP) scenarios –RCP4.5 and RCP8.5– using an ensemble of models. The average phenological dates during the period under study were, April 16th ± 6.6 days and April 5th ± 6.0 days for budbreak, May 31st ± 6.0 days and May 27th ± 5.3 days for flowering, July 26th ± 5.6 days and July 25th ± 5.8 days for veraison, and Ago 23rd ± 10.8 days and Ago 17th ± 9.0 days for harvest, respectively, for Tempranillo and Chardonnay. The projected changes in temperature imply an average change in the maximum growing season (April-August) temperatures of 1.2 and 1.9°C by 2050, and 1.6 and 2.6°C by 2070, under the RCP4.5 and RCP8.5 scenarios, respectively. A reduction in precipitation is predicted, which vary between 15% for 2050 under RCP4.5 scenario and up to 30% by 2070 under RCP8.5. The advance of the phenological dates for 2050, could be of 6, 7, 7, and 8 days for Tempranillo and 4, 6, 6 and 9 days for Chardonnay, respectively for budbreak, flowering, veraison and harvest under the RCP4.5 scenario. Under the RCP8.5 emission scenario, the advance could be up to 30% higher.