IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Factors influencing the production of the antioxidant hydroxytyrosol during alcoholic fermentation: Yeast Assimilable Nitrogen and Sugar content.

Factors influencing the production of the antioxidant hydroxytyrosol during alcoholic fermentation: Yeast Assimilable Nitrogen and Sugar content.

Abstract

Hydroxytyrosol (HT) is well known for its potent antioxidant activity and anticarcinogenic, antimicrobial, cardioprotective and neuroprotective properties. One possible explanation to its origin in wines is the synthesis from tyrosol, which in turn is produced from the Ehrlich pathway by yeasts.  This work aims to explore the factors that could increase the final content as the initial concentration of yeast assimilable nitrogen (YAN) and sugar. Two different concentrations of YAN were proved between 210mg/L and 300 mg/L. Additionally, two different concentrations of sugar were used: 100g/L and 240 g/L.  Alcoholic fermentations in synthetic must were performed with the strain QA23. Commercial Saccharomyces cerevisiae yeasts QA23 were used, as well as a strain with a specific modification to increase the production of fusel alcohols. Experimental design includes different YAN and sugar concentrations. The analysis was performed in a Thermo Scientific liquid chromatography system consisting of a binary UHPLC Dionex Ultimate 3000RS, connected to a quadrupole orbitrap Qexactive hybrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany), which was equipped with a heated electrospray ionization probe (HESI-II). The column used was a ZORBAX SB-C18 (2.1 × 100 mm, 1.8-μm particle size) Higher concentrations of hydroxytyrosol were obtained in synthetic must with higher amount of sugar (240g/L) and lesser YAN (210mg/L). Furthermore, the modified yeast presents a higher capacity to produce HT and tyrosol. Selecting the adequate conditions of sugar and YAN can increase the HT and tyrosol content 10 times.These results might explain certain differences between HT content in wines.  

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Garcia-Parrilla Maria del Carmen1, González-Ramírez Marina1, Guillamón José M.2, Valero Eva3, Cerezo Ana B.1, Troncoso Ana M.1 and Garcia-Parrilla M. Carmen1

1Universidad de Sevilla
2Instituto de Agroquímica y Tecnología de Alimentos
3Universidad Pablo de Olavide

Contact the author

Keywords

hydroxytyrosol, UHPLC, mass spectrometry, yeast, fermentation.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Sustainable management of grapevine trunk diseases

Grapevine trunk diseases (GTD) occur wherever grapes are grown and are considered the main biotic factor reducing yields and shortening vineyards’ lifespan. Currently, no product is available to eradicate GTD once grapevines are infected. Therefore, prophylactic strategies based on pruning wound protection and ‘remedial surgery’, the only eradication method based on the elimination of infected wood and renewal of the vine by means of new canes or suckers, are the only effective strategies available. The Canadian grape and wine industry focusses on a sustainable production and thus, looking for alternatives to chemicals for disease management is a top priority.

Chitosan treatment to manage grapevine downy mildew

Downy mildew is one of the most important grapevine diseases, caused by the Oomycete Plasmopara viticola. The management of the disease in organic agriculture can require up to 15 copper applications per year. However, copper accumulates in the soil, is phytotoxic and is toxic for organisms living in the soil, its use has been restricted in European Union to maximum 28 kg in 7 years. Therefore, testing of alternatives with equal effectiveness is desirable. Among those, the natural biopolymer chitosan, obtained from crab shells, proved to be effective toward downy mildew in plot experiments. The aim of our trials was to extend chitosan applications in large scale experiments in different years, cultivars and environmental conditions.

Construction of a 3D vineyard model using very high resolution airborne images

In recent years there has been a growth in interest and number of research studies regarding the application of remote optical and thermal sensing by unmanned aerial vehicle (UAV) in agriculture and viticulture. Many papers report on the use of images to map or estimate the growth and water status of plants, or the heterogeneity of different parcels. Most often, NDVI or other similar indices are used.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Stem growth disorder and xylem anatomy modifications during esca pathogenesis in grapevines

Esca is a grapevine vascular disease with detrimental consequences on vineyard yield and longevity. Recently, esca leaf symptom development has been shown to result in the occlusion of xylem vessels by tyloses in leaves and stems, leading to hydraulic failure. However, little is known regarding the response of xylem anatomy and stem growth to esca in different varieties . Here we studied the impact of esca leaf symptom development on grapevine physiology, stem growth, and xylem anatomy in two widespread cultivars, Cabernet sauvignon and Sauvignon blanc.