IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Abstract

Ochratoxin A (OTA) is a mycotoxin produced by several filamentous fungi infecting grape bunches (Penicillium and Aspergillus spp.), this toxin pass to must when grapes are crushed and later it is found in wine. Following the evaluations of the toxicity of OTA, European Commission Regulations have been promulgated introducing upper limits for OTA concentrations in various commodities (cereals, cereal products, dried vine fruit, coffee, wine, grape juice, baby foods and dietary foods for special medical purposes). The use of fungal (Plerotus eryngii, Pleurotus pulmonarius and Trametes versicolor), and bacterial (Streptomyces coelicor) laccases permits to decrease Ochratoxin A, and other mycotoxins in buffer systems containing various natural and artificial redox mediators. Recently several laccases from lactic acid bacteria of wine and other foods have been isolated, identified and characterized. The aim of this research was to study the effect of synthetic and natural mediators on the degradation of Ochratoxin A (OTA) using laccases from Botrytis cinerea (fungal) and lactic acid bacteria. Studies were carried out in acetate buffer and model wine and evaluated the effect of different oenological factors (pH, SO2 and ethanol content). Quantification of OTA was accomplished by LC-QTOF analysis. Results showed that fungal and bacterial laccases alone were not effective in decreasing OTA content and the presence of redox mediators was required to achieve some reduction of OTA concertation, both in buffer and model wine.  Monomeric flavanols catechin and epicatechin were the most effective mediators among those assayed, followed by ferulic acid. Caftaric acid and the flavonols quercetin and quercetin-3-O-rutinoside were almost ineffective as mediators. SO2 at a concentration of 30 mg/L was able to completely prevent OTA degradation. These preliminary results confirmed the activity of laccase enzymes against ochratoxin A and provide knowledge on the effects of natural redox mediators suggesting new biological alternative strategies to eliminate undesirable substances present in wine.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Gómez-Alonso Sergio1, Martínez Tania Paniagua1, Pérez-Navarro José2, Olmeda Isidoro3, Pardo Isabel3, Ferrer Sergi3, Canals Joan Miquel4 and Zamora Fernando4

1Faculty of Chemical Sciences and Technologies, University of Castilla-La Mancha
2Higher Technical School of Agronomic Engineering, University of Castilla-La Mancha
3Faculty of Biological Sciences, University of Valencia
4Faculty of Oenology, Rovira i Virgili University

Contact the author

Keywords

ochratoxin A, laccase, fungal, lactic acid bacteria, phenolic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Ripening potential of Touriga Nacional variety with different canopy management techniques and in different regions (Dão, Bairrada and Vinhos Verdes)

Foreseeing climatic changes, the abnormally hot and dry year of 2005 can be revealer of some varieties behavior in different climatic conditions.

Analysis of primary, secondary and tertiary aromas in Vitis vinifera L. Syrah wines with an extemporaneous production cycle in two regions of São Paulo – Brazil, using GC-MS

The aromatic perception is one of the main factors that influence the
consumer when determining the wine’s quality and acceptance. Numerous factors (soil, climate,
winemaking style, cultivar) can influence the volatile compounds. Some of these compounds are released directly from the grape berries while others are formed during the fermentation and aging processes. However, little is known about the quality and aromatic formation of Syrah variety in the winter cycle cultivated in São Paulo.

Sustainable wine industry challenge: optimised cork powder us new sustainable fining agent to remove negative volatile phenols

AIM: Cork, the bark of Quercus suber L. is a natural, renewable, sustainable, and biodegradable raw material, representing an abundant and cheap source of raw material. Portugal is the major cork producer (185,000 tons) processing about three-quarters of the world’s cork, generating up to 25 wt % of cork dust as a by-product.

Rapid optical method for tannins estimation in red wines

In this work, an innovative analytical method has been proposed for fast and reliable in-line analysis of tannins in wines; the method is fast, does not require sample preparation and is based on the selective reactivity of tannins in a mixture containing proteinaceous matter (i.e. gelatin), under pH 3.5, resulting in the formation of white cloudiness.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.