IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Abstract

Ochratoxin A (OTA) is a mycotoxin produced by several filamentous fungi infecting grape bunches (Penicillium and Aspergillus spp.), this toxin pass to must when grapes are crushed and later it is found in wine. Following the evaluations of the toxicity of OTA, European Commission Regulations have been promulgated introducing upper limits for OTA concentrations in various commodities (cereals, cereal products, dried vine fruit, coffee, wine, grape juice, baby foods and dietary foods for special medical purposes). The use of fungal (Plerotus eryngii, Pleurotus pulmonarius and Trametes versicolor), and bacterial (Streptomyces coelicor) laccases permits to decrease Ochratoxin A, and other mycotoxins in buffer systems containing various natural and artificial redox mediators. Recently several laccases from lactic acid bacteria of wine and other foods have been isolated, identified and characterized. The aim of this research was to study the effect of synthetic and natural mediators on the degradation of Ochratoxin A (OTA) using laccases from Botrytis cinerea (fungal) and lactic acid bacteria. Studies were carried out in acetate buffer and model wine and evaluated the effect of different oenological factors (pH, SO2 and ethanol content). Quantification of OTA was accomplished by LC-QTOF analysis. Results showed that fungal and bacterial laccases alone were not effective in decreasing OTA content and the presence of redox mediators was required to achieve some reduction of OTA concertation, both in buffer and model wine.  Monomeric flavanols catechin and epicatechin were the most effective mediators among those assayed, followed by ferulic acid. Caftaric acid and the flavonols quercetin and quercetin-3-O-rutinoside were almost ineffective as mediators. SO2 at a concentration of 30 mg/L was able to completely prevent OTA degradation. These preliminary results confirmed the activity of laccase enzymes against ochratoxin A and provide knowledge on the effects of natural redox mediators suggesting new biological alternative strategies to eliminate undesirable substances present in wine.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Gómez-Alonso Sergio1, Martínez Tania Paniagua1, Pérez-Navarro José2, Olmeda Isidoro3, Pardo Isabel3, Ferrer Sergi3, Canals Joan Miquel4 and Zamora Fernando4

1Faculty of Chemical Sciences and Technologies, University of Castilla-La Mancha
2Higher Technical School of Agronomic Engineering, University of Castilla-La Mancha
3Faculty of Biological Sciences, University of Valencia
4Faculty of Oenology, Rovira i Virgili University

Contact the author

Keywords

ochratoxin A, laccase, fungal, lactic acid bacteria, phenolic compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

WHICH IMPACT FOR PROANTHOCYANIDIC TANNINS ON RED WINE FRUITY AROMA? SENSORY AND PHYSICOCHEMICAL APPROACHES

Previous research on the fruity character of red wines highlighted the role of esters. Literature provides evidence that, besides these esters, other compounds that are not necessarily volatiles may have an important impact on the overall aroma of wine, contributing to a modulation of its global aromatic expression. The goal of this work was to assess the olfactory consequences of a mixture between esters and proanthocyanidic tannins, through sensory and physico-chemical approaches. Sensory analysis of numerous aromatic reconstitutions, including triangular tests, detection thresholds, and sensory profiles, were conducted in order to evaluate the sensory impact of tannins on red wine esters perception.

Switch genes as a key to understand the grapevine ripening disorder berry Shrivel?

The ripening of grapevine berries encompasses complex morphological and physiological processes, especially at veraison. Berry shrivel (BS) is a ripening physiological disorder affecting grape berries with visible symptoms appearing short after veraison. The main symptoms of BS are a strong reduction in sugar accumulation, inhibited anthocyanin biosynthesis and high pH values. The most popular red grape cultivar in Austria “Blauer Zweigelt” (Vitis vinifera L.) is specifically prone to develop the BS ripening disorder and up to date a no specific cause or causes could be identified. Recently omics approaches have identified and characterized key processes during grapevine ripening. Among them a small subset of genes, called SWITCH, have been described as markers for the onset of the ripening process in fruits.

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

Harvest dates, climate, and viticultural region zoning in Greece

Climate is clearly one of the most important factors in the success of all agricultural systems, influencing whether a crop is suitable to a given region, largely controlling crop production and quality, and ultimately driving economic sustainability. Today many assessments of a region’s climate comes from a combination of station and spatial climate data analyses that facilitate the evaluation of the general suitability for viticulture and potential wine styles, allows for comparisons between wine regions, and offers growers a measure of assessing appropriate cultivars and sites.

Effect of environmentally friendly vineyard protection strategies on yeast ecology during fermentation

AIM: Currently, an increasing concern from governments and consumers about environmental sustainability of wine production provides new challenges for innovation in wine industry. Accordingly, the application of more-environmentally friendly vineyard treatments against fungal diseases (powdery and downy mildew) could have a cascading impact on yeast ecology of wine production.