WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 1 - WAC - Posters 9 Antioxidant activity of grape seed and skin extract during ripening

Antioxidant activity of grape seed and skin extract during ripening

Abstract

Reactive oxygen species (ROS) play an important physiological role in the body’s defense and being involved in numerous signaling pathways 1, 2. When the balance between oxidant and antioxidant species is altered in favor of ROS, oxidative stress is generated. In this condition the cells are damaged as the ROS oxidize important cellular components, such as proteins, lipids, nucleic acids and carbohydrates. The damage caused at the cellular level has repercussions on a systemic level favoring the appearance of various chronic and inflammatory diseases, such as cancer, cardiovascular diseases, type 2 diabetes and Alzheimer’s disease 3, 4. A way to keep the equilibrium in the organism is the intake of alimentary antioxidants that work synergistically with the endogenous ones to keep the good state of health. Dietary polyphenols are one of the most important groups of natural antioxidants, they are secondary metabolites found in fruits, vegetables, cereals, tea, wine. Grape is known to be one of the richest foods in polyphenols and the antioxidant activity of the grape is directly related to the phenolic concentration and composition, which varies during the ripening. In this work the antioxidant activity of grape seed and skin extracts of the red varieties Syrah, Tannat and Merlot during the ripening were evaluated through in vitro assays. The reference method Folin-Ciocalteu was used for the determination of the total polyphenol content (TPC) and the total antioxidant capacity (TAC) was determined through the radical scavenging assay using 1.1 -diphenyl-2-picrylhydrazyl (DPPH).5 The results of the analyses show that the seed extracts were richer in polyphenols than the skin extracts and had the highest antioxidant potential for all the grape ripening stages. The highest TPC and TAC values were found at the green stage for skins, at veraison for seeds.

[1] P. Tai, M. Ascoli, Reactive oxygen species (ROS) play a critical role in the cAMP-induced activation of Ras and the phosphorylation of ERK1/2 in Ledydig cells. Molecular endocrinology 25 (2011) 885-893.

[2] M. Valko, D. Leibfritz, J. Moncol, M. T. D. Cronin, M. Mazur, J. Telser, Free radicals and antioxidants in normal physiological function and human disease. The International Journal of Biochemistry & Cell Biology 39,1 (2007) 44-84.

[3] Grune, R. Shringarpure, N. Sitte, K. Davies, Age-Related changes in protein oxidation and proteolysis in mammalian cells. Journal of Gerontology, 56A (2001), B459-B467.

[4] N. Noguchi, E. Niki, Phenolic antioxidants: A rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radical Biology & Medicine, 28 (2000), 1538-1546.

[5] V.S. Chedea, R. M. Pop, Total polyphenol content and antioxidant DPPH assays on biological samples. In R. R. Watson (Ed.), Polyphenols in plants (2019) 169-183.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Adriana Capozzi, Luca Garcia, Karen Lambert, Cédric Saucier

Presenting author

Adriana Capozzi – SPO, Université de Montpellier, INRAe, Montpellier SupAgro, 34000 Montpellier, France

SPO, Université de Montpellier, INRAe, Montpellier SupAgro, 34000 Montpellier, France | PhyMedExp, Université de Montpellier, INSERM U1046, UMR CNRS, 9412, Montpellier, France | SPO, Université de Montpellier, INRAe, Montpellier SupAgro, 34000 Montpellier, France

Contact the author

Keywords

skin-seeds-antioxidant activity-polyphenols-grape

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

A fine scale study of temperature variability in the Saint-Emilion area (Bordeaux, France)

As the quality and typicity of wine are influenced by the climate, it is essential to have a good knowledge of climate variability, especially with regard to temperature, which has a great impact on vine behavior and grape ripening.

ASSESSMENT OF ‘DOLCETTO’ GRAPES AND WINES FROM DIFFERENT AREAS OF OVADA DOCG

Dolcetto (Vitis vinifera L.) is one of the traditionally cultivated varieties in Piedmont (north-east Italy). Dolcetto wines have long been associated with local consumption and they are little known internationally. In particular, the Ovada area (south-east Piedmont), even if it represents a small share of the regional PDO Dolcetto production, is one of the oldest and vocated territory, giving wine also suitable for aging. In this study, the basic composition and phenolic content of Dolcetto grapes for Ovada DOCG wines have been investigated in three different vintages (2020-2022), as well as the main aspects of the derived commercial and experimental wines (basic parameters, phenolics, volatile compounds, sensory properties).

Managing extraction of colour, tannin and methoxypyrazines in Pinot noir grapes treated by leaf removal

Managing extraction of tannins and green aroma compounds attributed from methoxypyrazines in winemaking is crucial for producing high quality Pinot noir wine. This study1 investigated the impact of leaf removal on concentrations of anthocyanins, tannins, and methoxypyrazines in Pinot noir grapes and resultant wines.

Estudio de fertilidad en variedades blancas en Castilla-la Mancha

La adaptación de nuevas variedades a zonas de cultivo fuera de su área de origen presenta múltiples interrogantes. En Castilla-La Mancha se está produciendo en los últimos años una gran inquietud por la diversificación y la reconversión de variedades.

Utilizing ozone for the management of powdery mildew (Erysiphe necator Schwein.) in vineyards: potential and challenges

Powdery mildew, caused by fungal pathogens, poses a significant threat to grapevines in the DOCa Rioja region. In efforts to improve control strategies while reducing reliance on conventional phytosanitary products, ozone could constitute a potential alternative. However, it has short persistence, thus requiring frequent treatments. This study aimed to assess the suitability of ozone as an active substance for controlling powdery mildew within a phytosanitary strategy aimed at reducing conventional phytosanitary product usage. The strategy integrating ozone with conventional products yielded powdery mildew levels comparable to conventional treatments in both disease incidence and severity.