WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Metabolomic study of mixed Saccharomyces cerevisiae yeast during fermentation

Metabolomic study of mixed Saccharomyces cerevisiae yeast during fermentation

Abstract

Alcoholic fermentation conducted by microorganism is a key step in the production of wine. In this process, interactions between different species of yeast are widely described but their mechanisms are still poorly understood. The interactions studied in wine are mainly between Saccharomyces and non-Saccharomyces species. Therefore, little is known about the mechanisms of interactions between Saccharomyces cerevisiae strains in mixed culture, yet they are major actors that are in part responsible for the metabolic modifications within each strain and therefore for the quality of the final product. In order to better understand interactions occurring between two strains of S. cerevisiae, pure cultures were compared with mixed co-cultures and blend of wines using ultra high-resolution mass spectrometry, LC-MS, GC-MS and sensory analysis. Three mixed were studied, on the same Chardonnay must, each involving a common strain. Ultrahigh -resolution mass spectrometry (uHRMS) revealed important differences between pure cultures and mixed cultures. This work reports that mixed fermentation led to changes in chemical wine composition. Besides, we found that the blends showed a different chemical composition than mixed cultures. This indicates that the co-culture did not consist of the addition of two independent yeast metabolisms but of interaction events.  We also observed that depending on the strain associated to the common strain, there were interaction phenomena of different natures. These findings were further demonstrated by the volatilome study of 65 volatile compounds and sensory analysis. Indeed, a modulation of the volatile composition and sensory profile were noted when both strains were combined but also according to the strains involved in the fermentation.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

FANNY BORDET, Rémy Romanet, Florian Bahut, Jordi Ballester, Cristina Peña, Régis Gougeon, Anne Julien-Ortiz, Philippe Schmitt Kopplin, Chloé Roullier-Gall, Hervé Alexandre

Presenting author

FANNY BORDET – Université Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon,France-IUVV, rue Claude Ladrey, BP 27877, 21078 Dijon CEDEX, France ; Lallemand SAS, 19 rue des Briquetiers, Blagnac CEDEX, France

Université Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon,France-IUVV, rue Claude Ladrey, BP 27877, 21078 Dijon CEDEX, France | Lallemand SAS, 19 rue des Briquetiers, Blagnac CEDEX, France | Centre des Sciences du Goût et de l’Alimentation, AgroSup Dijon, CNRS, INRA, Université Bourgogne – Franche-Comté, F-21000 Dijon, France | University of Zaragoza, Nutrition, Laboratorio de Análisis del Aroma y Enología (LAAE) Dpt. Química Analítica. Facultad de Ciencias, . 50009 Zaragoza. Spain | Université Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon,France-IUVV, rue Claude Ladrey, BP 27877, 21078 Dijon CEDEX, France | Lallemand SAS, 19 rue des Briquetiers, Blagnac CEDEX, France | German Research Center for Environmental Health, Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany | Université Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon,France-IUVV, rue Claude Ladrey, BP 27877, 21078 Dijon CEDEX, France | Université Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon,France-IUVV, rue Claude Ladrey, BP 27877, 21078 Dijon CEDEX, France,

Contact the author

Keywords

Saccharomyces cerevisiae – interactions – metabolomic – sensory analysis – volatiles compounds

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

POTENTIAL OF PEPTIDASES FOR AVOIDING PROTEIN HAZES IN MUST AND WINE

Haze formation in wine during transportation and storage is an important issue for winemakers, since turbid wines are unacceptable for sale. Such haze often results from aggregation of unstable grape proteinaceous colloids. To date, foreseeably unstable wines need to be treated with bentonite to remove these, while excessive quantities, which are often required, affect the wine volume and quality (Cosme et al. 2020). One solution to avoid these drawbacks might be the use of peptidases. Marangon et al. (2012) reported that Aspergillopepsins I and II were able to hydrolyse the respective haze-relevant proteins in combination with a flash pasteurisation. In 2021, the OIV approved this enzymatic treatment for wine stabilisation (OIV-OENO 541A and 541B).

Effect of cytokinin and auxin application on double cropping performance in Vitis vinifera: preliminary findings

Double cropping is a novel technique, driven by the extension of the growing season caused by global warming.

Colloidal color stabilization in wine: A comparative study of Saccharomyces and non-Saccharomyces mannoproteins

Structure-function relationships between the polysaccharide part of S. cerevisiae Mannoprotein Pools (MPs) and their potential to interact with anthocyanins and Protein-Tannins aggregates was previously assessed [1,2].

Bioprotective non-Saccharomyces yeast as an alternative to sulfites for the winemaking process

Sulfur dioxide (SO2) is used in winemaking due of its antioxidant, antioxydasic and antiseptic properties. Excessive amount of SO2 can negatively impact wine sensory perception and be detrimental for health. Agri-food industries are more transparent towards consumers concerning addition of sulfites, and oenology is no exception in this clairvoyance. As a consequence, the increase of consumers preference for wine with low or absent of sulfites addition is notorious. In this context, the impact of low/zero sulfites winemaking process on the microbial community should be evaluated. Moreover, microbial agents corresponding to bioprotective cultures represent a growing interest as an alternative to sulfites preservation in the early stages of vinification. However, scientific studies conducted to demonstrate their real effect are almost rare.