GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Ozone treatment: a solution to improve sanitary and physiological quality of vine plant

Ozone treatment: a solution to improve sanitary and physiological quality of vine plant

Abstract

Context and purpose of the study – The vineyard world is faced to a lot of fungal diseases. Grapevine Trunk Diseases (GTD) are some of the major. After exhibiting chronical foliar symptoms, grapevines can die by apoplexy within only few days. A range species of fungi was described to be associated with the apparition of early symptoms of GTD. It is well known that ozone dissolved into water is a powerful disinfectant with no remanence. The main goal of this study was to test the efficiency of this process on different fungal species associated with GTD in vitro and in planta conditions.

Material and methods – In vitro sanitary tests: Eighteen strains of two different species associated with GTD were selected (Phaeomoniella chlamydospora and Phaeoacremonium minimum). Ozone dissolved into water (4.5 g.m-3 according the Henry’s law) or autoclaved demineralized water (control) were applied on spore suspensions from strains. Suspensions were then plated on agar medium. Germinating spores were observed after five days. In vivo sanitary tests:Cuttings of Vitis vinifera Cabernet-Sauvignon clone 15 were drilled until the vascular channels. In each injury, plants received 20 µL of spore suspension (105 spores.mL-1) of P. minimum. Immediately after inoculation, infected wounded damages were treated with 20 µL of ozone dissolved into water (4.5 g.m-3 according the Henry’s law). The fungal development was evaluated 4, 6 and 9 weeks after inoculation by q-PCR.

Results – In vitro sanitary tests: Solution of ozone dissolved into water presented a complete sporicide effect. Indeed, no spore germinated in ozonated treatments whereas water treated controls normally developed. In vivo sanitary tests:The anti-fungal abilities of ozone treatment were secondly assessed by quantification of P. minimum DNA in woody tissues (via qPCR). Four and six weeks after inoculation, ozone treatment strongly reduced the source of inoculum present in the injury, resulting in more of 50% decrease of the number of P. minimum copies per ng of total. After nine weeks, the quantity of DNA is more important in ozone modality than in control modality. These results suggest that ozone treatment slowed down the fungal colonization via its primary sporicide effect in cutting-wounding conditions. Finally, consequences on the physiological aspect of the plant after ozone treatment should be discussed during the congress thanks to some news results.

DOI:

Publication date: September 26, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Ana ROMEO OLIVAN1,ǂ, Marielle PAGÈS1, 2,*,ǂ, Coralie BRETON1, Frédéric VIOLLEAU2,3 and Alban JACQUES1

1 PPGV, INP-PURPAN, F-31076 Toulouse, France
2 Plateforme TOAsT, Université de Toulouse, INP-PURPAN, Toulouse, France
3 Laboratoire de Chimie Agro-industrielle, LCA, Université de Toulouse, INRA, 31000 Toulouse, France
ǂ These authors contributed equally to this work

Contact the author

Keywords

grapevine, fungi, ozone, disinfection, growth

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Downscaling of remote sensing time series: thermal zone classification approach in Gironde region

In viticulture, the challenges of local climate modelling are multiple: taking into account the local environment, fine temporal and spatial scales, reliable time series of climate data, ease of implementation and reproducibility of the method. At the local scale, recent studies have demonstrated the contribution of spatialization methods for ground-based climate observation data considering topographic factors such as altitude, slope, aspect, and geographic coordinates (Le Roux et al, 2017; De Rességuier et al, 2020). However, these studies have shown questions in terms of the reproducibility and sustainability of this type of climate study. In this context, we evaluated the potential of MODIS thermal satellite images validated with ground-based climate data (Morin et al, 2020). Previous studies have been encouraging, but questions remain to be explored at the regional scale, particularly in the dynamics of the massive use of bioclimatic indices to classify the climate of wine regions. The results at the local scale were encouraging, but this approach was tested in the current study at the regional scale. Several objectives were set: 1) to evaluate the downscaling method for land surface temperature time series, 2) to identify regional thermal structure variations. We used weekly minimum and maximum surface temperature time series acquired by MODIS satellites at a spatial resolution of 1000 m and downscaled at 500 m using topographical variables. Two types of analyses were performed:

Short-term relationships between climate and grapevine trunk diseases in southern French vineyards

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Ecophysiological performance of Vitis rootstocks under water stress

The use of rootstocks tolerant to soil water deficit is an interesting strategy to cope with limited water availability. Currently, several nurseries are breeding new genotypes, but the physiological basis of its responses under water stress are largely unknown. To this end, an ecophysiological assessment of the conventional 110-Richter (110R) and SO4, and the new M1 and M4 rootstocks was carried out in potted ungrafted plants. During one season, these Vitis genotypes were grown under greenhouse conditions and subjected to two water regimes, well-watered and water deficit. Water potentials of plants under water deficit down to < -1.4 MPa, and net photosynthesis (AN) <5 μmol m-2 s-1 did not cause leaf oxidative stress damage compared to well-watered conditions in any of the genotypes. The antioxidant capacity was sufficient to neutralize the mild oxidative stress suffered. Under both treatments, gravimetric differences in daily water use were observed among genotypes, leading to differences in the biomass of root, shoot and leaf. Under well-watered conditions, SO4 and 110R were the most vigorous and M1 and M4 the least. However, under water stress, SO4 exhibited the greatest reduction in biomass while M4 showed the lowest. Remarkably, under these conditions, SO4 reached the least negative stem water potential (Ψstem), while M1 reduced stomatal conductance (gs) and AN the most. In addition, SO4 and M1 genotypes also showed the highest and lowest hydraulic conductance values, respectively. Our results suggest that there are differences in water use regulation among genotypes, not only attributed to differences in stomatal regulation or intrinsic water use efficiency at the leaf level. Therefore, because no differences in canopy-to-root ratio were achieved, it is hypothesized that xylem vessel anatomical differences may be driving the reported differences among rootstocks performance. Results demonstrate that each Vitis rootstock differs in its ecophysiological responses under water stress.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)