terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic identification of 200-year-old Serbian grapevine herbarium

Genetic identification of 200-year-old Serbian grapevine herbarium

Abstract

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions. Obtaining DNA from more than 100 years old plant remains requires the use of ancient (or archive) DNA (aDNA) extraction procedures, adapted to aDNA conditions (typically degraded, damaged, and contaminated with environmental DNA). Here, we used a protocol for recovering ultrashort DNA molecules from herbarized leaves in facilities tailored for aDNA extraction. In 84 of 103 samples, DNA was successfully extracted and quantified. The presence of grapevine DNA in these extractions was verified in 31 randomly selected samples by partially amplifying a Vitis psaA chloroplast gene. Then, genotyping with 13 SSR was performed in a laboratory where grapevine DNA had never been amplified. The success of the genotyping analyses varied, from a third of the samples producing no positive results, to others working well, like modern DNA samples. The genetic profiles obtained were then compared to those stored in international databases (ICVV and VIVC). The samples were also genotyped for 48 SNPs and confirmed the SSR results. Up to 32 of the herbarized samples were successfully identified as 19 different varieties from the Western Balkans and neighboring regions, such as cv. ‘Kadarka Kek’, indicating their continuous cultivation for more than 200 years. The combined analysis of ancient and modern samples allows elucidating the historical evolution of the crop in Serbia.

Acknowledgements: Ministry of Agriculture, Forestry, and Water Management, Republic of Serbia; and European COST Action CA17111 INTEGRAPE.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Royo, Carolina1,2*; Tello, Javier1; Rat, Milica3; Ferradás, Yolanda1,4; Nikolić, Miroslav5; Sabovljević, Aneta6; Todić, Slavica7; Ivanišević, Dragoslav8; Posth, Cosimo9; Weigel, Detlef10; Peña-Chocarro, Leonor2; Grbić, Miodrag1,6,11; Martínez-Zapater, José Miguel1; Tomanović, Željko6; Ibáñez, Javier1

1Instituto de Ciencias de la Vid y del Vino (ICVV, CSIC-CAR-UR), Departamento de Viticultura, Logroño, Spain
2Instituto de Historia, Departamento de Arqueología y Procesos Sociales, Madrid, Spain
3University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia
4Universidad de Santiago de Compostela, Santiago de Compostela, Spain
5University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia
6University of Belgrade, Faculty of Biology, Belgrade, Serbia
7University of Belgrade, Faculty of Agriculture, Department of Viticulture, Belgrade, Serbia
8University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
9University of Tuebingen, Archaeo- and Palaeogenetics, Tuebingen, Germany
10Max-Plank Institute for Biology, Molecular Biology, Tuebingen, Germany
11University of Western Ontario, Department of Biology, London, Canada

Contact the author*

Keywords

ancient DNA, herbarium, genotyping, grapevine, microsatellite, SNP

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Viticultural heritage in mountain territories of Catalonia: prospecting in the region of Osona, northern Spain

The recovery of ancestral or minority vine varieties has been gaining great interest in recent years, among other reasons because it is likely that some of these varieties, due to the fact that they are found in relict areas, have a greater potential for adaptation to external factors (biotic or abiotic) and can minimize the effects that climate change is causing in viticulture. Varieties that can be grown at altitude are currently being sought to combat rising temperatures and prolonged extreme drought conditions. In Catalonia, the Pyrenean expansion of vineyard cultivation is documented from the 10th century and has been related to the “small climatic optimum” (9th-12th centuries) and also to seigniorial power.[1] But different adverse climatic periods and the arrival of Phylloxera by the late 19th century made many of these crops disappear.[2]

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).

Sugar accumulation disorder Berry Shrivel – from current knowledge towards novel hypothesis

In contrast to fruit and grape berry ripening, the biological processes causing ripening disorders are often much less understood, although shriveling disorders of fruits are manifold and contribute to yield losses and reduced fruit quality worldwide. Shrinking berries are a common feature for all shriveling disorders in grapevine although their timing of appearance during the berry ripening process and their underlying induction processes distinct them from each other. The sugar accumulation disorder Berry Shrivel (BS) is characterized by a suppression of sugar accumulation short after veraison resulting in berries low in sugar content and anthocyanins in berry skins, while the organic acid content is similar. Recent studies analyzed the biochemical, morphological and molecular processes affected in BS berries and linked early changes to the period of ripening onset [1,2].

Combined use of leaf removal and natural shading to delay grape ripening in Manto negro (Vitis vinifera L.) under deficit irrigation 

The increasingly frequent heat waves during grape ripening pose challenges for premium wine grape production. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality.

Chemical profiling and sensory analysis of wines from resistant hybrid grape cultivars vs conventional wines

Recently, there has been a shift toward sustainable wine production, according to EU policy (F2F and Green Deal), to reduce pesticide usage, improve workplace health and safety, and prevent the impacts of climate change. These trends have gained the interest of consumers and winemakers. The cultivation of disease resistant hybrid grape cultivars (DRHGC), known as ‘PIWI’ grapes can help with these objectives [1]. This study aimed to profile white and red wines produced from DRHGC in South Tyrol (Italy). Wines produced from DRHGCs were compared with conventional wines produced by the same wineries. The measured parameters were residual sugars, organic acids, alcohol content, pigments and other phenolics by LC-QqQ/MS, colorimetric indexes (CIELab); and volatile profiles (HS-SPME-GCxGC-ToF/MS [2]).