terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic identification of 200-year-old Serbian grapevine herbarium

Genetic identification of 200-year-old Serbian grapevine herbarium

Abstract

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions. Obtaining DNA from more than 100 years old plant remains requires the use of ancient (or archive) DNA (aDNA) extraction procedures, adapted to aDNA conditions (typically degraded, damaged, and contaminated with environmental DNA). Here, we used a protocol for recovering ultrashort DNA molecules from herbarized leaves in facilities tailored for aDNA extraction. In 84 of 103 samples, DNA was successfully extracted and quantified. The presence of grapevine DNA in these extractions was verified in 31 randomly selected samples by partially amplifying a Vitis psaA chloroplast gene. Then, genotyping with 13 SSR was performed in a laboratory where grapevine DNA had never been amplified. The success of the genotyping analyses varied, from a third of the samples producing no positive results, to others working well, like modern DNA samples. The genetic profiles obtained were then compared to those stored in international databases (ICVV and VIVC). The samples were also genotyped for 48 SNPs and confirmed the SSR results. Up to 32 of the herbarized samples were successfully identified as 19 different varieties from the Western Balkans and neighboring regions, such as cv. ‘Kadarka Kek’, indicating their continuous cultivation for more than 200 years. The combined analysis of ancient and modern samples allows elucidating the historical evolution of the crop in Serbia.

Acknowledgements: Ministry of Agriculture, Forestry, and Water Management, Republic of Serbia; and European COST Action CA17111 INTEGRAPE.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Royo, Carolina1,2*; Tello, Javier1; Rat, Milica3; Ferradás, Yolanda1,4; Nikolić, Miroslav5; Sabovljević, Aneta6; Todić, Slavica7; Ivanišević, Dragoslav8; Posth, Cosimo9; Weigel, Detlef10; Peña-Chocarro, Leonor2; Grbić, Miodrag1,6,11; Martínez-Zapater, José Miguel1; Tomanović, Željko6; Ibáñez, Javier1

1Instituto de Ciencias de la Vid y del Vino (ICVV, CSIC-CAR-UR), Departamento de Viticultura, Logroño, Spain
2Instituto de Historia, Departamento de Arqueología y Procesos Sociales, Madrid, Spain
3University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia
4Universidad de Santiago de Compostela, Santiago de Compostela, Spain
5University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia
6University of Belgrade, Faculty of Biology, Belgrade, Serbia
7University of Belgrade, Faculty of Agriculture, Department of Viticulture, Belgrade, Serbia
8University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
9University of Tuebingen, Archaeo- and Palaeogenetics, Tuebingen, Germany
10Max-Plank Institute for Biology, Molecular Biology, Tuebingen, Germany
11University of Western Ontario, Department of Biology, London, Canada

Contact the author*

Keywords

ancient DNA, herbarium, genotyping, grapevine, microsatellite, SNP

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

Effects of different soil types and soil management on greenhouse gas emissions 

Soil is important in the carbon cycle and the dynamics of greenhouse gases (CO2, CH4 and N2O). Key soil characteristics, such as organic matter content, texture, structure, pH and microbial activity, play a determining role in GHG emissions[1]. The objective of the study is to delimit different types of soil, with different soil management and to be able to verify the differences in CO2, CH4 and N2O emissions. The study was carried out in a vineyard of Bodegas Campo Viejo in Logroño (La Rioja), whose plant material is Vitis vinifera L. cv. Tempranillo.

Limiting magnesium availability: a novel approach to managing brettanomyces spoilage in winemaking

Brettanomyces is a world-renowned yeast that negatively impacts the chemical composition of wines through the production of metabolites that negatively impact the sensory properties of the final product. Its resilience in wine conditions and ability to produce off-flavors make it a challenge for winemakers. Currently, the primary control technique involves adding sulfur dioxide (SO2); however, some Brettanomyces strains are developing resistance to this preservative agent. [1] Therefore, new management strategies are necessary to control this spoilage yeast.

Evaluation of the effects of pruning methodology on the development of young vines 

Grapevine pruning is one of the most important practices in the vineyards. Winegrowers use it to provide the vines the shape needed, or to maintain it once achieved, and also to balance vegetative growth and fruit production. In the last decades, careless pruning has been blamed, among other factors, as responsible of the vineyard decay that is been observed even in young vines. However, to our knowledge, there is a lack of systematic research trying to elucidate to which extent the pruning method used affects plant development or its susceptibility to grapevine trunk diseases (GTD). Within this context, the aim of this work is to study the influence of different pruning method strategies on the development of field-planted young vines.

Grapevine cane pruning extract enhances plant physiological capacities and decreases phenolic accumulation in canes and leaves 

Vine cane extracts are a valuable byproduct due to their rich content of polyphenols, vitamins, and other beneficial compounds, which can affect and benefit the vine and the grapes. This study aims to evaluate the response of grapevine plants to irrigation with water supplemented with a vine cane extract, both at physiology response and phenolic composition in different parts of the plant (root, trunk, shoot, leaf, and berry).
Cane extract was obtained by macerating crushed pruning residues with warm water (5:1) and pectolytic enzymes. Two-year-old potted plants were irrigated with water (Control) while others were irrigated with cane extracts, either at 1:4 (w/v, cane extract/water; T 1:4) or at 1:8 (w/v, cane extract/water; T 1:8).