terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic identification of 200-year-old Serbian grapevine herbarium

Genetic identification of 200-year-old Serbian grapevine herbarium

Abstract

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions. Obtaining DNA from more than 100 years old plant remains requires the use of ancient (or archive) DNA (aDNA) extraction procedures, adapted to aDNA conditions (typically degraded, damaged, and contaminated with environmental DNA). Here, we used a protocol for recovering ultrashort DNA molecules from herbarized leaves in facilities tailored for aDNA extraction. In 84 of 103 samples, DNA was successfully extracted and quantified. The presence of grapevine DNA in these extractions was verified in 31 randomly selected samples by partially amplifying a Vitis psaA chloroplast gene. Then, genotyping with 13 SSR was performed in a laboratory where grapevine DNA had never been amplified. The success of the genotyping analyses varied, from a third of the samples producing no positive results, to others working well, like modern DNA samples. The genetic profiles obtained were then compared to those stored in international databases (ICVV and VIVC). The samples were also genotyped for 48 SNPs and confirmed the SSR results. Up to 32 of the herbarized samples were successfully identified as 19 different varieties from the Western Balkans and neighboring regions, such as cv. ‘Kadarka Kek’, indicating their continuous cultivation for more than 200 years. The combined analysis of ancient and modern samples allows elucidating the historical evolution of the crop in Serbia.

Acknowledgements: Ministry of Agriculture, Forestry, and Water Management, Republic of Serbia; and European COST Action CA17111 INTEGRAPE.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Royo, Carolina1,2*; Tello, Javier1; Rat, Milica3; Ferradás, Yolanda1,4; Nikolić, Miroslav5; Sabovljević, Aneta6; Todić, Slavica7; Ivanišević, Dragoslav8; Posth, Cosimo9; Weigel, Detlef10; Peña-Chocarro, Leonor2; Grbić, Miodrag1,6,11; Martínez-Zapater, José Miguel1; Tomanović, Željko6; Ibáñez, Javier1

1Instituto de Ciencias de la Vid y del Vino (ICVV, CSIC-CAR-UR), Departamento de Viticultura, Logroño, Spain
2Instituto de Historia, Departamento de Arqueología y Procesos Sociales, Madrid, Spain
3University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia
4Universidad de Santiago de Compostela, Santiago de Compostela, Spain
5University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia
6University of Belgrade, Faculty of Biology, Belgrade, Serbia
7University of Belgrade, Faculty of Agriculture, Department of Viticulture, Belgrade, Serbia
8University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
9University of Tuebingen, Archaeo- and Palaeogenetics, Tuebingen, Germany
10Max-Plank Institute for Biology, Molecular Biology, Tuebingen, Germany
11University of Western Ontario, Department of Biology, London, Canada

Contact the author*

Keywords

ancient DNA, herbarium, genotyping, grapevine, microsatellite, SNP

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Genetic variation among wild grapes native to Japan

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan. A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan.

Long-lasting flavour perception of wines treated with oenological additives considering the individual PROP taste-phenotype

The use of oenological additives is becoming a common practice due to the technological and sensory properties they provide to the wines. However, the number of studies focused on the impact that these additives might induce on wine flavor perception during wine tasting is still quite scarce. The aim of this work was to evaluate the effect of three different types of common oenological additives: two oenotannins (ellagitannin and gallotannin) and a commercial preparation of yeast mannoproteins on the long-lasting flavor perception (aroma and astringency).

Organic mulches slightly influence wine phenolic composition and sensorial properties

Grapevines have traditionally been grown in semi-arid areas, but viticulture is now compromised by climate change. Therefore, it is necessary to implement environmentally friendly viticulture practices to adapt grapevines to current climatic conditions. In this context, organic mulches offer many benefits, such as reduced soil erosion and increased organic matter, soil water content and crop productivity. However, these practices must not compromise grape and wine quality. Therefore, the objective of this study was to evaluate the effect on wine physicochemical and phenolic composition and sensorial properties of different soil management practices on the vine row. Over four years, five soil treatments were examined in two different vineyards.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Qualitative and productive characterization of a minority variety: ‘Branco lexítimo’ in DO Ribeira Sacra (Spain)

The actual climate changes, together with the strong regulation of the European Union and Spanish government, in search of sustainable viticulture, have forced the recovery of minority varieties, expanding the range of grape varieties, as well as the possible development of wines with unique profiles. In the Ribeira Sacra DO (Spain), a comparative study of the agronomic and qualitative behavior of the ‘Branco lexítimo’ variety has been carried out, compared to the majority white variety in the DO: ‘Godello’, located in the same study plot, with identic soil and climatic conditions. The study contemplated the analysis of phenology and leaf water potential, as well as the productive results and the analysis of the must quality, during four seasons: 2018 – 2021.