terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic identification of 200-year-old Serbian grapevine herbarium

Genetic identification of 200-year-old Serbian grapevine herbarium

Abstract

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions. Obtaining DNA from more than 100 years old plant remains requires the use of ancient (or archive) DNA (aDNA) extraction procedures, adapted to aDNA conditions (typically degraded, damaged, and contaminated with environmental DNA). Here, we used a protocol for recovering ultrashort DNA molecules from herbarized leaves in facilities tailored for aDNA extraction. In 84 of 103 samples, DNA was successfully extracted and quantified. The presence of grapevine DNA in these extractions was verified in 31 randomly selected samples by partially amplifying a Vitis psaA chloroplast gene. Then, genotyping with 13 SSR was performed in a laboratory where grapevine DNA had never been amplified. The success of the genotyping analyses varied, from a third of the samples producing no positive results, to others working well, like modern DNA samples. The genetic profiles obtained were then compared to those stored in international databases (ICVV and VIVC). The samples were also genotyped for 48 SNPs and confirmed the SSR results. Up to 32 of the herbarized samples were successfully identified as 19 different varieties from the Western Balkans and neighboring regions, such as cv. ‘Kadarka Kek’, indicating their continuous cultivation for more than 200 years. The combined analysis of ancient and modern samples allows elucidating the historical evolution of the crop in Serbia.

Acknowledgements: Ministry of Agriculture, Forestry, and Water Management, Republic of Serbia; and European COST Action CA17111 INTEGRAPE.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Royo, Carolina1,2*; Tello, Javier1; Rat, Milica3; Ferradás, Yolanda1,4; Nikolić, Miroslav5; Sabovljević, Aneta6; Todić, Slavica7; Ivanišević, Dragoslav8; Posth, Cosimo9; Weigel, Detlef10; Peña-Chocarro, Leonor2; Grbić, Miodrag1,6,11; Martínez-Zapater, José Miguel1; Tomanović, Željko6; Ibáñez, Javier1

1Instituto de Ciencias de la Vid y del Vino (ICVV, CSIC-CAR-UR), Departamento de Viticultura, Logroño, Spain
2Instituto de Historia, Departamento de Arqueología y Procesos Sociales, Madrid, Spain
3University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia
4Universidad de Santiago de Compostela, Santiago de Compostela, Spain
5University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia
6University of Belgrade, Faculty of Biology, Belgrade, Serbia
7University of Belgrade, Faculty of Agriculture, Department of Viticulture, Belgrade, Serbia
8University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
9University of Tuebingen, Archaeo- and Palaeogenetics, Tuebingen, Germany
10Max-Plank Institute for Biology, Molecular Biology, Tuebingen, Germany
11University of Western Ontario, Department of Biology, London, Canada

Contact the author*

Keywords

ancient DNA, herbarium, genotyping, grapevine, microsatellite, SNP

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The exploitation of Croatian grapevine genetic resources for the breeding of new resistant cultivars 

Croatian viticulture is mainly based on native grapevine varieties susceptible to various diseases and pests, which leads to unsustainable use of large amounts of pesticides. The sustainable development of viticulture in the future will only be possible by increasing the resistance of the grapevine through the development of new resistant varieties. Breeding programs have been launched in the leading wine-growing countries to develop resistant varieties possessing high-quality levels. Native cultivars from Croatia are not included in the breeding programs of other countries.

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

The tolerance of grapevine rootstocks to water deficit is related to root morphology and xylem anatomy traits 

Climate change is altering water balances, thereby compromising water availability for crops. In grapevine, the strategic selection of genotypes more tolerant to soil water deficit can improve the resilience of the vineyard under this scenario. Previous studies demonstrated that root anatomical and morphological traits determine vine performance under water deficit conditions. Therefore, 13 ungrafted rootstock genotypes, 6 commercial (420 A, 41 B, Evex 13-5, Fercal, 140 Ru y 110 R), and 7 from new breeding programs (RG2, RG3, RG4, RG7, RG8, RG9 and RM2) were evaluated in pots during 2021 and 2022.

Effect of riboflavin on the longevity of white and rosé wines

Light is a fundamental part at sales points which influences in the conservation of wines, particularly in those that are sold in transparent glass bottles such as rosé wines and increasingly white wines. The photochemical effect known as “light-struck taste” can cause changes in the aromatic characteristics of the wine. This “light-struck taste” is due to reactions triggered by the photochemical sensitivity of riboflavin (RBF).

Assessing the Effectiveness of Electrodialysis in Controlling Brettanomyces Growth in Wine

Brettanomyces yeast can negatively impact the quality and stability of wines, posing a significant challenge to winemakers. [1] This study aims to develop novel management practices to limit Brettanomyces impact on wines by evaluating the effectiveness of electrodialysis (ED) technology in removing magnesium (Mg2+) from wine to prevent the development of Brettanomyces yeast. The ED technique utilizes charged membranes to extract ions from the wine, and it is considered an alternative to cold stabilization that requires less energy. [2]