terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of foliar application of urea and nano-urea on the cell wall of Monastrell grape skins

Effect of foliar application of urea and nano-urea on the cell wall of Monastrell grape skins

Abstract

The foliar application of urea has been shown to be able to satisfy the specific nutritional needs of the vine as well as to increase the nitrogen composition of the must. On the other hand, the use of nanotechnology could be of great interest in viticulture as it would help to slow down the release of urea and protect it against possible degradation. Several studies indicate that cell wall synthesis and remodeling are affected by nitrogen availability.

The study was carried out over three years (2019, 2020 and 2021), the effect on the main components of the cell wall of Monastrell grape skins was compared to control after foliar treatments with urea and nano-urea. The treatments, in triplicate, were manually applied with a spray dispenser.

The proportion of isolated cell wall was increased in both treatments every year. This rise would indicate a thickening of the cell walls. Proteins were enhanced in 2019 and 2021 in grapes from the two treatments, whereas in 2020 they were not modified. Phenolic compounds were not affected in 2019, decreasing in the nano-urea treatment in 2020 and in the urea treatment in 2021. Cellulose was diminished in 2019 in the nano-urea treatment and in both treatments in 2021, but was not affected in 2020. Hemicellulose was increased for both treatments in both 2019 and 2021, but only for urea in 2020. Finally, uronic acids were lower compared to control for both treatments in 2019, but raise in the two subsequent years.

In conclusion, the cell wall was modified by both treatments, which may have implications on its rigidity and therefore on the extraction of the compounds of interest present in the grape skins. The treatments applied in the form of nano-urea had comparable effects to those obtained with urea in the conventional way, despite being applied at a significantly lower dose.

Acknowledgements: To all the staff of Estación Enológica de Jumilla

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

María José Giménez-Bañón1*, Diego Fernando Paladines-Quezada1, Juan Daniel Moreno-Olivares1, Belén Parra-Torrejón2, Juan Antonio Bleda-Sánchez1, Gloria-Belén Ramírez-Rodríguez2, José Manuel Delgado-López2, Rocío Gil-Muñóz1

1Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental
2Departamento de Química Inorgánica. Facultad de Ciencias. Universidad de Granada

Contact the author*

Keywords

nanotechnology, nitrogen, fertilization, protein, phenolic-compound, cellulose, uronic-acids

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.

Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Grapevine rootstocks provide protection against environmental biotic and abiotic stresses. Nitrogen (N) and iron (Fe) are growth-limiting factors in many crop plants due to their effects on the chlorophyll and photosynthetic characteristics. Iron nutrition of plants can be significantly affected by different nitrogen forms through altering the uptake ratio of cations and anions, and changing rhizosphere pH. The aim of this study was to investigate the response mechanisms of grapevine rootstocks due to the interaction between different nitrogen forms and iron uptake.

Unraveling the complexity of high-temperature tolerance by characterizing key players of heat stress response in grapevine

Grapevine (Vitis spp.) is greatly influenced by climatic conditions and its economic value is therefore directly linked to environmental factors. Among these factors, temperature plays a critical role in vine phenology and fruit composition. In such conditions, elucidating the mechanisms employed by the vine to cope with heat waves becomes urgent. For the past few years, our research team has been producing molecular and metabolic data to highlight the molecular players involved in the response of the vine and the fruit to high temperatures [1]. Some of these temperature-sensitive genes are currently undergoing characterization using transgenesis approaches coupled or not with genome editing, taking advantage of the Microvine genotype [2].

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.
We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin.

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.