terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Abstract

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices. Our goal was to investigate the potential benefits of organic viticulture and cover cropping on the quality and biodiversity of vineyard soils. The soil samples were collected to a depth of 20 cm, and the vineyards were categorized based on their pest and soil management strategies. Employing specific primers and following the Illumina amplicon protocol, we conducted sequencing on the Illumina MiSeq platform (2×300 bp). The resulting data underwent bioinformatics analysis utilizing Qiime2 and the SILVA v138.1 database to explore biodiversity measures and differentially abundant taxa. Over 80 taxonomic groups (genus/family) of nematodes were identified and utilized for calculating nematode-based indices using the NINJA platform. Findings showed no significant differences between cover cropping and tilling practices but for pest management. Thus, organic viticulture increased the α-biodiversity of soil nematodes, and nematode-based indices revealed raised environmental disturbance, higher occurrence of plant-parasitic nematodes of adverse implications for crop health, and declined soil food web structure in IPM vineyards. In conclusion, this approach appears well suited to assess vineyard soil health.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Rubén Blanco-Pérez1*, María de Toro2, Sara Sánchez-Moreno3, Sergio Álvarez-Ortega4, Alícia Pou1, Raquel Campos-Herrera1

1 Instituto de Ciencias de la Vid y del Vino (CSIC-Univ. de La Rioja-Gobierno de La Rioja), Logroño, Spain.
Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, Spain.
Universidad Rey Juan Carlos, Madrid, Spain.

Contact the author*

Keywords

bioindicators, cover cropping, DNA-metabarcoding, pest management, tillage

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Typicality of Rioja wines: identification of sensory profiles for the three subregions of DOCa Rioja

Within the DOCa Rioja three main production areas are differentiated: Rioja Alta (RA), Rioja Alavesa (RAv) and Rioja Oriental (RO). They are three diverse territories with particular characteristics that are claimed to give rise to differentiated profiles. The present work aims at evaluating the sensory diversity of young commercial red wines in these three subregions. Therefore 30 young red wines (mainly Tempranillo and vintage 2021), ten from each subregion, were sensory described following a non-verbal free sorting task and a verbal free comment task by 32 well-established Rioja winemakers.

Effect of riboflavin on the longevity of white and rosé wines

Light is a fundamental part at sales points which influences in the conservation of wines, particularly in those that are sold in transparent glass bottles such as rosé wines and increasingly white wines. The photochemical effect known as “light-struck taste” can cause changes in the aromatic characteristics of the wine. This “light-struck taste” is due to reactions triggered by the photochemical sensitivity of riboflavin (RBF).

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

The combined use of Lachancea thermotolerans and lactic bacteria in wine technology

The production of most red wines that are sold involves an alcoholic fermentation carried out by yeasts of the Saccharomyces genus, and a subsequent fermentation carried out by lactic bacteria of the Oenococus oeni species after the first one is fully completed. However, the traditional process can face complications, which can be more likely in grape juices with high levels of sugar and pH. Because of climate change, these situations are more frequent in the wine industry. The main hazards in those scenarios are halts or delays in the alcoholic fermentation or the growth of unwanted bacteria while the alcoholic fermentation is not done yet and the wine still has residual sugars.

Time vs drought: leaf age rather than drought drives osmotic adjustment in V. vinifera cv. Pinot Noir

Global warming and increased frequency and/or severity of drought events are among the most threatening consequences of climate change for agricultural crops. In response to drought, grapevine (as many other plants) exhibits osmotic adjustment through active accumulation of osmolytes which in turn shift the leaf turgor loss point (TLP) to more negative values, allowing to maintain stomata opened at lower water potentials1. We investigated the capacity of Pinot noir leaves to modulate their osmotic potential as a function of: (i) time (seasonal osmoregulation), (ii) growing temperatures, and (iii) drought events, to enhance comprehension of the resilience of grapevines in drought conditions. We performed trails under semi-controlled field conditions, and in two different greenhouse chambers (20/15 °C vs 25/20 °C day/night). For two consecutive vegetative seasons, grafted potted grapevines (Pinot noir/SO4) were subjected to two different water regimes for at least 30 days: well-watered (WW) and water deficit (WD).