terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Abstract

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices. Our goal was to investigate the potential benefits of organic viticulture and cover cropping on the quality and biodiversity of vineyard soils. The soil samples were collected to a depth of 20 cm, and the vineyards were categorized based on their pest and soil management strategies. Employing specific primers and following the Illumina amplicon protocol, we conducted sequencing on the Illumina MiSeq platform (2×300 bp). The resulting data underwent bioinformatics analysis utilizing Qiime2 and the SILVA v138.1 database to explore biodiversity measures and differentially abundant taxa. Over 80 taxonomic groups (genus/family) of nematodes were identified and utilized for calculating nematode-based indices using the NINJA platform. Findings showed no significant differences between cover cropping and tilling practices but for pest management. Thus, organic viticulture increased the α-biodiversity of soil nematodes, and nematode-based indices revealed raised environmental disturbance, higher occurrence of plant-parasitic nematodes of adverse implications for crop health, and declined soil food web structure in IPM vineyards. In conclusion, this approach appears well suited to assess vineyard soil health.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Rubén Blanco-Pérez1*, María de Toro2, Sara Sánchez-Moreno3, Sergio Álvarez-Ortega4, Alícia Pou1, Raquel Campos-Herrera1

1 Instituto de Ciencias de la Vid y del Vino (CSIC-Univ. de La Rioja-Gobierno de La Rioja), Logroño, Spain.
Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, Spain.
Universidad Rey Juan Carlos, Madrid, Spain.

Contact the author*

Keywords

bioindicators, cover cropping, DNA-metabarcoding, pest management, tillage

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Culturable microbial communities associated with the grapevine soil in vineyards of La Rioja, Spain

The definition of soil health is complex due to the lack of agreement on adequate indicators and to the high variability of global soils. Nevertheless, it has been widely used as synonymous of soil quality for more than one decade, and there is a consensus warning of scientists that soil quality and biodiversity loss are occurring due to the traditional intensive agricultural practices.
In this work we monitored a set of soil parameters, both physicochemical and microbiological, in an experimental vineyard under three different management and land use systems: a) addition of external organic matter (EOM) to tilled soil; b) no tillage and plant cover between grapevine rows, and c) grapevines planted in rows running down the slope and tilled soil.

Aromatic characterization of Moscato Giallo by GC-MS/MS and stable isotopic ratio analysis of the major volatile compounds

Among the Moscato grapes, Moscato Giallo is a winegrape variety characterized by a high content of free and glycosylated monoterpenoids, which gives very aromatic wines. The aromatic bouquet of Moscato Giallo is strongly influenced by the high concentration of linalool, geraniol, linalool oxides, limonene, α-terpineol, citronellol, HO-trienol, HO-diols, 8-Hydroxylinalool, geranic acid and β-myrcene, that give citrus, rose, and peach notes.

Use of UHPH to improve the implantation of non-Saccharomyces yeasts

Ultra High-Pressure Homogenization (UHPH) is a high-pressure pumping at 300 MPa (>200 MPa) with a subsequent depressurization against a highly resistant valve made of tungsten carbide covered by ceramic materials or carbon nanoparticles. The intense impact and shear efforts produce the nano-fragmentation of colloidal biopolymers including the elimination of microorganism (pasteurization or sterilization depending on in-valve temperature) and the inactivation of enzymes.

Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Crops are facing increasing biotic and abiotic stress pressures due to global changes. However, trade-off mechanisms between these stresses and the underlying physiological processes are still poorly understood, especially in perennial crop species. To better understand these trade-offs, we studied the effect of drought on grapevine (Vitis vinifera) physiology and esca-related wood fungal communities. Esca is a vascular disease caused by a community of wood-infecting pathogenic fungi, and characterized by trunk necrosis, leaf scorch symptoms, yield losses, and mortality.

Polysaccharide families of lyophilized extracts obtained from unfermented varietal grape pomaces

The recovery of bioactive compounds from grape and wine by-products is currently an important objective for revaluation and sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds. The aim of this study was to evaluate the polysaccharide (PS) composition of extracts obtained from pomaces of different white and red grape varieties of Castilla y León. Grape pomaces were obtained after the pressing in the winemaking process.