terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Abstract

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices. Our goal was to investigate the potential benefits of organic viticulture and cover cropping on the quality and biodiversity of vineyard soils. The soil samples were collected to a depth of 20 cm, and the vineyards were categorized based on their pest and soil management strategies. Employing specific primers and following the Illumina amplicon protocol, we conducted sequencing on the Illumina MiSeq platform (2×300 bp). The resulting data underwent bioinformatics analysis utilizing Qiime2 and the SILVA v138.1 database to explore biodiversity measures and differentially abundant taxa. Over 80 taxonomic groups (genus/family) of nematodes were identified and utilized for calculating nematode-based indices using the NINJA platform. Findings showed no significant differences between cover cropping and tilling practices but for pest management. Thus, organic viticulture increased the α-biodiversity of soil nematodes, and nematode-based indices revealed raised environmental disturbance, higher occurrence of plant-parasitic nematodes of adverse implications for crop health, and declined soil food web structure in IPM vineyards. In conclusion, this approach appears well suited to assess vineyard soil health.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Rubén Blanco-Pérez1*, María de Toro2, Sara Sánchez-Moreno3, Sergio Álvarez-Ortega4, Alícia Pou1, Raquel Campos-Herrera1

1 Instituto de Ciencias de la Vid y del Vino (CSIC-Univ. de La Rioja-Gobierno de La Rioja), Logroño, Spain.
Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, Spain.
Universidad Rey Juan Carlos, Madrid, Spain.

Contact the author*

Keywords

bioindicators, cover cropping, DNA-metabarcoding, pest management, tillage

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Organic mulches slightly influence wine phenolic composition and sensorial properties

Grapevines have traditionally been grown in semi-arid areas, but viticulture is now compromised by climate change. Therefore, it is necessary to implement environmentally friendly viticulture practices to adapt grapevines to current climatic conditions. In this context, organic mulches offer many benefits, such as reduced soil erosion and increased organic matter, soil water content and crop productivity. However, these practices must not compromise grape and wine quality. Therefore, the objective of this study was to evaluate the effect on wine physicochemical and phenolic composition and sensorial properties of different soil management practices on the vine row. Over four years, five soil treatments were examined in two different vineyards.

Effect of foliar application of urea and nano-urea on the cell wall of Monastrell grape skins

The foliar application of urea has been shown to be able to satisfy the specific nutritional needs of the vine as well as to increase the nitrogen composition of the must. On the other hand, the use of nanotechnology could be of great interest in viticulture as it would help to slow down the release of urea and protect it against possible degradation. Several studies indicate that cell wall synthesis and remodeling are affected by nitrogen availability.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Combined abiotic-biotic plant stresses on the roots of grapevine

In the 19th century, devastating outbreaks of phylloxera (Daktulosphaira vitifoliae Fitch), almost brought European viticulture to its knees. Phylloxera does not only take energy in form of sugars from the vine, but also affects the up- and down- regulations of genes, acts as a carbon sink and reprograms the physiology of the grapevines, including nutrient uptake and the defense system [1]. A key trait of rootstocks is the ability to perform well under high lime conditions as about 30 % of the land surface has calcareous soil. Iron deficiency not only causes the well-known problems of lime-induced chlorosis and stunted growth, but also affects the entire plant metabolism.