terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Abstract

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices. Our goal was to investigate the potential benefits of organic viticulture and cover cropping on the quality and biodiversity of vineyard soils. The soil samples were collected to a depth of 20 cm, and the vineyards were categorized based on their pest and soil management strategies. Employing specific primers and following the Illumina amplicon protocol, we conducted sequencing on the Illumina MiSeq platform (2×300 bp). The resulting data underwent bioinformatics analysis utilizing Qiime2 and the SILVA v138.1 database to explore biodiversity measures and differentially abundant taxa. Over 80 taxonomic groups (genus/family) of nematodes were identified and utilized for calculating nematode-based indices using the NINJA platform. Findings showed no significant differences between cover cropping and tilling practices but for pest management. Thus, organic viticulture increased the α-biodiversity of soil nematodes, and nematode-based indices revealed raised environmental disturbance, higher occurrence of plant-parasitic nematodes of adverse implications for crop health, and declined soil food web structure in IPM vineyards. In conclusion, this approach appears well suited to assess vineyard soil health.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Rubén Blanco-Pérez1*, María de Toro2, Sara Sánchez-Moreno3, Sergio Álvarez-Ortega4, Alícia Pou1, Raquel Campos-Herrera1

1 Instituto de Ciencias de la Vid y del Vino (CSIC-Univ. de La Rioja-Gobierno de La Rioja), Logroño, Spain.
Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, Spain.
Universidad Rey Juan Carlos, Madrid, Spain.

Contact the author*

Keywords

bioindicators, cover cropping, DNA-metabarcoding, pest management, tillage

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Vines are exposed to environmental conditions that cause abiotic stress on the plants (drought, nutrient and mineral deficits, salinity, etc.). Polyamines are growth regulators involved in various physiological processes, as in abiotic plant stress responses. Stressful conditions can modify grape’s composition, and in this work, we have focused on studying the effect of abiotic stress on the composition of polyamines and amino acids in grapes. In addition, the effect of grape variety on these compounds has been studied.

Physico-chemical properties of vine pruning residues with potential as enological additive

Grapes are one of the world’s primary fruit crops, and pruning activities generate high amounts of annual wood wastes [1]. These pruning shoots contain valuable phenolic compounds and could have numerous potential applications [1,2]. Consequently, the aim of this work was to evaluate the physico-chemical properties of vine pruning residues with potential as enological additives. For this purpose, grapevine shoots from 12 varieties grown in Chile were collected during the winter of 2021.

Conventional and alternative pest management strategies: a comparative proteomic study on musts

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases.

Phenolic composition profile of cv. Tempranillo wines obtained from severe shoot pruning vines under semiarid conditions

One of the limitations of vineyards in warm areas is the loss of wine quality due to higher temperatures during the grape ripening period. In order to adapt the vineyards to these new climatic conditions, a possible solution is to delay the ripening process of the grapes towards periods with milder temperatures, by means of management practices and thus improve the quality of the fruit and the wine produced. The technique of severe shoot pruning (SSP) has proven useful in achieving this objective.