terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Abstract

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices. Our goal was to investigate the potential benefits of organic viticulture and cover cropping on the quality and biodiversity of vineyard soils. The soil samples were collected to a depth of 20 cm, and the vineyards were categorized based on their pest and soil management strategies. Employing specific primers and following the Illumina amplicon protocol, we conducted sequencing on the Illumina MiSeq platform (2×300 bp). The resulting data underwent bioinformatics analysis utilizing Qiime2 and the SILVA v138.1 database to explore biodiversity measures and differentially abundant taxa. Over 80 taxonomic groups (genus/family) of nematodes were identified and utilized for calculating nematode-based indices using the NINJA platform. Findings showed no significant differences between cover cropping and tilling practices but for pest management. Thus, organic viticulture increased the α-biodiversity of soil nematodes, and nematode-based indices revealed raised environmental disturbance, higher occurrence of plant-parasitic nematodes of adverse implications for crop health, and declined soil food web structure in IPM vineyards. In conclusion, this approach appears well suited to assess vineyard soil health.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Rubén Blanco-Pérez1*, María de Toro2, Sara Sánchez-Moreno3, Sergio Álvarez-Ortega4, Alícia Pou1, Raquel Campos-Herrera1

1 Instituto de Ciencias de la Vid y del Vino (CSIC-Univ. de La Rioja-Gobierno de La Rioja), Logroño, Spain.
Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, Spain.
Universidad Rey Juan Carlos, Madrid, Spain.

Contact the author*

Keywords

bioindicators, cover cropping, DNA-metabarcoding, pest management, tillage

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).

Possible methods of adaptation to the effects of climate change in the Tokaj Wine Region 

Viticulture’s adaptation to the harmful effects of climate change is globally the biggest challenge of the near future. Short, extremely intensive rainfalls and longer periods of drought are getting more frequent in the Tokaj Wine Region, where the majority of the vineyards are cultivated on steep slopes. Hence, erosion has high risk, especially when combined with the loess-based soils on about ten percent of the region. The environmentally beneficial cover crop and mulch usage can effectively reduce the risk of erosion, according to research done by the Tokaj Wine Region Research Institute of Viticulture and Oenology.

Effect of ultraviolet B radiation on pathogenic molds of grapes

The fungicidal effect of UV-C radiation (100-280 nm wavelength) is well known, but its applicability for the control of pathogenic molds of grapes is conditioned by its effect on the host and by the risks inherent in its handling[1].
As an alternative, the effect in vitro of UV-B radiation (280-315 nm) on the main pathogenic molds of grapes has been studied: Botrytis cinerea, Aspergillus niger, Penicillium expansum and Rhizopus stolonifer.

Irrigation frequency in four grapevine red varieties in Spain. Effect on must volatile composition

The irrigation water management in the vineyard is a crucial aspect to obtain sustainable quality production over time. Previous studies have set the water requirements to be applied in the vineyard at 30 % of the reference evapotranspiration (ET0), although there are no studies that settle the effects of the frequency of irrigation application on red varieties in Spain. The present study contemplates the application of deficit irrigation (30 % ET0) applying a weekly dose in a single irrigation (T07) or in two irrigation events (T03) per week. The study has been carried out in 2021-2022 with four red varieties in different Spanish wine regions: Garnacha Tinta (Badajoz), Tempranillo (Valladolid), Syrah (Albacete) and Mencía (Lugo). The effects of irrigation frequency on must volatile composition have been evaluated through GC-MS.

Phenotyping bud break and trafficking of dormant buds from grafted vine

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1].